Packed Column Application Guide

Packed Column GCApplication Guide1Bulletin 890APacked Column GCApplication GuideThis guide to packed column applications provides information on thebest approach to designing your application. It includes helpfulinformation about packings, phases, tubing, and different types ofdeactivations. Nearly 200 different applications are organized ac-cording to type of analysis. In most cases, the catalog number for thepacking is included with the application chromatogram.Key Wordsl packed column l gas chromatographyWhat Makes a Good Column?Tubing, support, and stationary phase are all that is needed to prepare acolumn, but to prepare a column that will provide the desired separation mayrequire much more. First, the proper materials must be selected; this can bethe most difficult part. Also, these ingredients must be combined in such amanner that optimum performance can be obtained; this can turn out to bemore than one bargains for Any compromise in materials or procedures yieldsa compromised performance.The Rohrschneider and McReynolds ConstantsAn important feature is the use of the Rohrschneider and McReynoldsConstants. Using these systems, most of the stationary phases now in use havebeen characterized by a series of constants. These constants provide theworker with a wealth of practical information to help solve problems in columnselection. For more information and a listing of McReynolds values, requestBulletin 880.The SupportThe support plays a critical role in several ways in the performance of thecolumn. First, it governs the efficiency of the column (narrowness of peaks).The structure of the support, and the manner in which it is coated, bothcontribute to the efficiency. Secondly, the wrong choice of support caninteract with the sample to cause the chromatographic peaks to tail, i.e., theycan be highly asymmetrical and consequently difficult or impossible tomeasure. Ideally, the support should not interact with the sample but, inpractice, this does occur. By careful selection of the support and conditions,one can minimize this problem.The tailing phenomenon is caused by active sites on the surface of the support.These sites are ones that can form a hydrogen bond; consequently samplesthat form a strong hydrogen bond tail badly. In practice, compounds such aswater, glycols, alcohols, acids, and amines tail severely while carbonylT195890A©1999 Sigma-Aldrich Co.2compounds such as esters, ketones, and aldehydes tail to a lesser degree.Hydrocarbons that do not hydrogen bond are not bothered by tailing.To eliminate or reduce the tailing problem one modifies the support surfaceby: 1) removing the active sites by acid and/or base washing, 2) modifying thesurface by silanization, or 3) covering the active sites with the stationary phasehaving polar functional groups in it.Acid washing is effective in removing mineral impurities from the supportsurface as well as miscellaneous extraneous material. Base washing does notimpart any special advantage to the support that is not obtained with a wellacid washed support. Acid washing by itself is not effective in reducing tailingbut it is recommended where a polar phase is used, such as the polyesters andpolyglycols.Silanization, particularly with dimethyldichlorosilane (DMCS) is very effectivein reducing tailing. Combined with acid washing and DMCS treatment, theresulting support is recommended for most columns. Silane treatment is avery difficult process to control and should not be attempted unless you wishto make a research project out of it. When silicone stationary phases are used,it is mandatory that an acid-washed and DMCS-treated support be used. Thesilicone stationary phases, particularly when used in the 1-5% level, are noteffective in deactivating the support and require a silane-treated support.SUPELCOPORT is recommended where an acid-washed and DMCS-treatedsupport is required.The third procedure for deactivation, using a polar stationary phase, requiresthat the phase contain functional groups such as an ester, an ether, a hydroxyl,and an amine group. These functional groups have strong hydrogen bondingcharacteristics and tie up the active sites on the support surface. These phasesdo not require a silanized support, although, when they are used at a level of5% or less, silanization can be useful. Normally we recommend using an acid-washed support with these phases.When analyzing acids it is necessary that the stationary phase contain an acidto deactivate the support. See Bulletin 856.When working with basic compounds such as amines, the stationary phasemust contain a base to deactivate the support; otherwise severe tailing willresult. KOH is frequently used at a 1-2% level for the purpose. A basicstationary phase such as polyethyleneimines also may be used.Most of the GC supports in current use are made from diatomaceous earth,also called diatomite. The diatomite is processed in several ways producingtwo basic types of supports. These are conveniently recognized by their color.The particle size of supports are generally expressed in terms of screenopenings since screens are normally used to prepare them. The particle sizesnormally used in GC are as follows:60/80 mesh 250-177 microns80/100 mesh 177-149 microns100/120 mesh 149-125 micronsThe designation 60/80 mesh means that the particles have passed through a60 mesh screen (-60) and will not pass through the 80 mesh screen (+80). Itthen means that the particles are between 250-177 microns in size. Thecolumn efficiency improves with decreasing particle size. At present the80/100 mesh is the most popular size, but 100/120 mesh is used withincreasing frequency when more efficiency columns are desired.3Column TubingGlass, stainless steel, aluminum, and copper are the tubing materials com-monly used for columns. While glass is the most inert of the tubing, stainlesssteel is the most widely used.Glass is used in situations where the sample might interact with the walls ofthe tube. It is standard operating procedure to use glass columns whenworking with pesticides and biochemicals such as steroids and hormones.Glass is more inert then the metals and rarely causes tailing or decompositionof the samples. Glass columns are also used generally in situations where it isdesirable that the sample be injected directly into the column. An alternativeis to use a glass lined injection port to avoid the contact with metal since metalin the inlet can destroy certain samples or cause tailing. Glass columns alsopermit you to see how well a column has been packed.Fused silica lined stainless steel tubing is a suitable substitute for glass tubing.When properly deactivated, it meets or exceeds the deactivation of conven-tional deactivated glass columns.Metal columns are used where glass is not required. Stainless steel is generallyconsidered more inert than aluminum or copper. We recommend stainlesssteel as the metal of choice because it is easier to prepare columns of higherefficiency with stainless steel than with aluminum or copper. The hardness ofthe material appears to be important in transmitting the vibrational energywhen the column is vibrated or tapped. However, if in doubt . . . use glass. . . and silane treat it (All Supelco glass columns are silane-treated.)SP-Alloy T-1, our nickel alloy, approaches glass in inertness. Most newerinstruments are designed to handle 1/8" OD metal columns. When glasscolumns are to be used, the instruments are usually equipped to handle 1/4"OD columns. Here the glass column can be made with a heavy glass wallcutting down considerably on the problem of breakage. Where 1/8" OD glasscolumns are used, the breakage problems are usually severe.From our own experience we recommend using 2mm ID rather than 4mm IDglass columns because of the inherently higher efficiency. If you shift from4mm to 2mm columns, remember to reduce the carrier gas flow rate to 1/4,not 1/2. A reasonable flow rate for 4mm columns is 80mL/min, while for 2mmcolumns a good rate is 20mL/min.Upper Temperature LimitEach stationary phase has an upper temperature limit above which the columnshould not be operated. Most stationary phases are polymers that consist ofmaterials having a range of molecular weights. As the column temperatureis increased, the more volatile portion of the polymer is swept out of thecolumn by the carrier gas. The volatile products could also be formed bythermal degradation of the stationary phase while the column is being used.This is called bleed, and is seen on the recorder as a rise in the baseline or asnoise. Above the maximum upper limit the bleed rate is very high and thecolumn will have a relatively short life. In some cases the bleed rate is so highthat it will not be possible to move the recorder pen off of full scale. Note thatthe reported maximum upper limits are approximate values. Each columnproduced is shipped with a conditioning sheet, and minimum/maximumoperating temperature is indicated. For a listing of phases and their maxi-mum/minimum operating temperatures, refer to our general catalog.4Conditioning the ColumnBefore a column is used for the first time, it must be conditioned for a periodof hours to rid it of the very volatile portion of the stationary phase and alsothe last traces of solvent which were used in the coating step.We recommend conditioning the column at the maximum isothermal columntemperature which you expect to reach. One can start to use a column after ithas been conditioned at that temperature after only 3-4 hours, but condition-ing overnight is usually better. We recommend that you always condition thecolumn with a flow of carrier gas. Others have recommended “no-flow’conditioning for such materials as UC W98 silicone. This might be necessaryfor technical grade silicone, but chromatographic grade silicones such as theSPTM and OV® phases, and most other phases, should not be subjected to thisdrastic treatment.Many stationary phases are subject to oxidation and should not be exposedto air while hot. At all times when the column is hot, the carrier gas shouldbe allowed to flow through it. Cutting back on the carrier gas overnight canbe detrimental to the column. It may also be necessary to insert an oxygenscrubber in the carrier gas line.The Lower Temperature LimitObserving the lower temperature limit is also important to obtain goodcolumn performance. Some stationary phases are solids and are ineffective asa stationary phase in this form. They must be operated at a temperature abovetheir melting point.Selecting a ColumnThe simplest method of obtaining a column to handle a particular problem isto duplicate one that is referred to in the applications pages of this guide.Another method is to use the literature as a guide and then to substitute someof the newer, improved stationary phases and solid supports. If you cannotfind the solution to your separation problem in the literature, the problem ismore complex. When ordering the column, it is essential that you list all of thefollowing:a. the stationary phaseb. the supportc. the mesh range of the supportd. the treatment given the supporte. the percent coating of stationary phasef. the tube composition, i.e., glass, stainless, etc.g. the dimensions of the tube, i.e., length and diameter, both OD and ID ifpossibleh. the configuration of the column or instrument it is to be used withExample: 3% SP-2100 on 100/120 mesh SUPELCOPORTTM,6' x 1/4" OD, 2mm ID, Glass for HP6890.For a complete listing of our current line of columns, packings,and accessories, please refer to our general catalog.5Index to ChromatogramsCompound Class PageAcetaldehyde ...................................................................................... 6Acids .................................................................................................. 6Acids, Methyl Esters (also see Fatty Acid Methyl Esters) ...................... 11Alcohols ............................................................................................ 13Amines .............................................................................................. 19Amino Acids ...................................................................................... 21Aroclors ............................................................................................. 22Ammonia .......................................................................................... 24Aromatics .......................................................................................... 24Chlorinated Hydrocarbons ................................................................. 28Creosote ............................................................................................ 31Drugs ................................................................................................ 33EPA Methods (600 Series) .................................................................. 37Ethylene Oxide and Residues ............................................................. 46Fatty Acid Methyl Esters ..................................................................... 47Fragrances ......................................................................................... 51Freons ............................................................................................... 53Formaldehyde ................................................................................... 54HydrocarbonsC1-C2 Hydrocarbons & Permanent Gases ................................ 55C1-C3 Hydrocarbons ................................................................ 59C1-C4 Hydrocarbons ................................................................ 60C1-C5 Hydrocarbons ................................................................ 60C1-C6 Hydrocarbons ................................................................ 62Permanent Gases and Hydrocarbons.................................................. 64Pesticides .......................................................................................... 68Phenols ............................................................................................. 69Pheromones ...................................................................................... 71Simulated Distillation ......................................................................... 72Solvents ............................................................................................ 78Sugars ............................................................................................... 84Sulfur and Mercaptans ....................................................................... 85Steroids ............................................................................................. 92Sterols ............................................................................................... 94Triglycerides ...................................................................................... 95Index to ChromatogramsTrademarksAroclor — Monsanto Co.Bentone — National Lead Co., Baroid Sales Div.Carbopack — Sigma-Aldrich Co.Carbotrap — Sigma-Aldrich Co.Carbowax — Union Carbide Corp.Carboxen* — Sigma-Aldrich Co.Cellosolve — Union Carbide Corp.Chromosorb – Celite Corp.Dexsil — Dexsil Chemical Corp.Fluorcol — Sigma-Aldrich Co.Freon — E.I. du Pont de Nemours & Co., Inc.Hall — Tracor Instruments, Austin, Inc.HayeSep — Hayes Separations Inc.ORBO — Sigma-Aldrich Co.Oronite — Uniroyal, Inc.OV – Ohio Valley Specialty Chemical CompanyPetrocol — Sigma-Aldrich Co.Qualmix — Sigma-Aldrich Co.Scott — Scott Specialty Gases, Inc.SP – Sigma-Aldrich Co.SUPELCOPORT – Sigma-Aldrich Co.Supelpak — Sigma-Aldrich Co.Teflon — E.I. du Pont de Nemours & Co., Inc.TightSpec — Sigma-Aldrich Co.UCON – Union Carbide CorporationValco — Valco Instruments Co., Inc.*US Pat. No. 4,839,331.6795-0077795-0144Figure 1. Trace Acetaldehyde in Air (2500ppm)Packing: Hayesep® D, 100/120 meshCat. No.: 10293 (packing)Column: 3' x 1/8" ID stainless steelOven: 100°CCarrier: helium, 30cc/minDet.: P.E. 900 TC, 225 ma, 140°CInj.: Valco® valve, 100mL, 140°CFigure 2. Volatile Fatty Acids (C1-C7)Packing: 10% SP-1000/1% H3PO4 on 100/120 Chromosorb® W AWCat. No.: 11841 (packing)Column: 6' x 4mm ID glassOven: Fisher Model 2400, 147°CCarrier: helium, 86mL/minDet.: TCInj.: 14µL0 42 6 8 10 14 16 22 241345678 910MinFor more information, request Bulletin 856.1. Acetic acid2. Formic acid3. Propionic acid4. Isobutyric acid5. n-Butyric acid6. Isovaleric acid7. n-Valeric acid8. Isocaproic acid9. n-Caproic acid10. Heptanoic acid212For more information, request Bulletins 790 and 816.Chromatogram provided courtesy of Hayes Separations, Inc.20Min3211. Air2. Water3. Acetaldehyde (vol. 2500ppm)7795-0076795-0078For more information, request Bulletin 856.1. Formic acid2. Acetic acid3. Propionic acid4. Isobutyric acid5. n-Butyric acid6. Isovaleric acid7. n-Valeric acid8. Isocaproic acid9. n-Caproic acid10. Heptanoic acidFigure 3. Volatile Acid Standard Mix (C1-C7)Packing: 15% SP-1220/1% H3PO4 on 100/120 Chromosorb W AWCat. No.: 12144 (packing)Column: 6' x 4mm ID glassOven: Fisher Model 2400, 145°CCarrier: helium, 70mL/minDet.: TCInj.: 14µLFigure 4. Volatile Fatty Acids in Sewage Effluents(ppm Levels)Packing: GP 60/80 Carbopack® C/0.3% Carbowax® 20M/0.1% H3PO4Cat. No.: 11825-U (packing,15g/bottle)Column: 30" x 4mm ID glassOven: 120°CCarrier: nitrogen, 50mL/minDet.: FID, 200°CInj.: 1µL water containing 50ppmeach analyte, 200°CAcetaldehyde/Acids0 2 4 6 8 10 12 20 22Min8 97564321 10Useful for analyses of sewage treatmenteffluents and other wastewater.For trace analyses, use a glass column.For more information, request Bulletin 856.1. Acetic acid2. Propionic acid3. Isobutyric acid4. n-Butyric acid5. 2-Methylbutyric acid6. 3-Methylbutyric acid7. n-Valeric acid0 4 12Min141086275643218Figure 5. Free Fatty Acids (C2-C10)Packing: GP 10% SP-1200/1% H3PO4 on 80/100 Chromosorb W AWCat. No.: 11965 (packing, 20g/bottle)Column: 6' x 1/8" stainless steelOven: 125°C to 175°C at 15°C/minCarrier: nitrogen, 20mL/minDet.: FID, 200°CInj.: 0.5µL carbondisulfide containing0.1% each analyte,150°C1. C22. C33. iC44. C45. iC56. C57. C68. C89. C101236548791 3 5 6 17 1810Min713-0963795-0079For more information, request Bulletin 856. 0 2 4 6 8 10 12Min1234657 8For more information,request Bulletin 856.1. Myristic acid (C14:0)2. Palmitic acid (C16:0)3. Palmitoleic acid (C16:1)4. Stearic acid (C18:0)5. Oleic acid (C18:1)6. Linoleic acid (C18:2)7. Arachidic acid (C20:0)8. Linolenic acid (C18:3)Figure 6. Free Fatty Acids (C14:0 to C18:3)Packing: 5% DEGS-PS on100/120 SUPELCOPORTCat. No.: 11870-U (packing)Column: 3' x 2mm ID glassOven: 200°CCarrier: nitrogen, 20mL/minDet.: FID, 210°CInj.: 1µL Qualmix™ FA9713-0962Figure 7. Whole Milk Free AcidsPacking: 10% SP-216-PS on 100/120 SUPELCOPORTCat. No.: 11879 (packing)Column: 3' x 2mm ID glassDet: FID, 200°COven: 130°C to 200°C at 15°C/minCarrier: nitrogen, 20mL/minInj.: 0.5µL carbon disulfide containing 0.1% each analyte, 150°CAcidsConc. (ppm)U Unknown1. Acetic acid 3702. Propionic acid 783. Isobutyric acid 2824. Butyric acid 1125IS Trimethylacetic acid (int. std.) 505. 2-Methylbutyric acid 2206. Isovaleric acid 2847. Lactic acid 7108. Valeric acid 4650 4 8 12 16 20Min1 2 3 4UUIS5 678UU12 3547981011 120 2 4 6 8 10Min1. C4:02. C6:03. C8:04. C10:05. C12:06. C14:07. C16:08. C16:19. C18:010. C18:111. C18:212. C18:36795-0080For more information, request Bulletin 856.Figure 8. Lactic Acid in Corn Silage (C2-C5)Packing: 80/120 Carbopack B-DA/4% Carbowax 20MCat. No.: 11889 (packing)Column: 2m x 2mm ID TightSpec™ glass(stock column available)Oven: 175°CCarrier: nitrogen, 24mL/minDet.: FID, 200°CInj.: 1µL aqueous mixture, 200°CFor more information, request Bulletin 856.10713-096412 345678 9 100 5 10 15 20Min1. Methyl ethyl ketone (int. std.)2. Acetic acid3. Propionic acid4. Acrylic acid (5000ppm)5. Isobutyric acid6. Butyric acid7. Trimethylacetic acid (int. std.)8. 2-Methyl butyric acid9. Isovaleric acid10. Valeric acidFigure 9. Lactic Acid in Rumen FluidPacking: 80/120 Carbopack B-DA/4% Carbowax 20MCat. No.: 11889 (packing)Column: 2m x 2mm ID TightSpec glass(stock column available)Oven: 175°CCarrier: nitrogen, 24mL/minDet.: FID, 200°CInj.: 1µL aqueous mixture, 200°CFigure 10. Acrylic/Propionic Separation in C2-C5 AcidsPacking: 80/120 Carbopack B-DA/4% Carbowax 20MCat. No.: 11889 (packing,15g/bottle)Column: 2m x 2mm IDTightSpec glass(stock column available)Oven: 175°CCarrier: nitrogen, 24mL/minDet.: FID, 200°CInj.: 1µL water, 10ppm eachacid, 100ppm each internal standard, 200°CConc. (ppm)U Unknown1. Acetic acid 13002. Propionic acid 10603. Isobutyric acid 434. Butyric acid 313IS Trimethylacetic acid (int. std.) 505. 2-Methylbutyric acid 606. Isovaleric acid 417. Valeric acid 70Min40 8 12 16 20U 3421IS567UUFor more information, request Bulletin 856.For more information, request Bulletin 856.795-0082Lacticacid(9ppm)11Figure 11. Nonvolatile Methylated AcidsPacking: 10% SP-1000/1% H3PO4 on100/120 Chromosorb W AWCat. No.: 11841 (packing)Column: 6' x 4mm ID glassOven: 155°CCarrier: helium, 86mL/minFigure 12. N-TFA-O-Methyl Esters of D- & L-ValineAmino AcidsPacking: 5% SP-300 on 100/120 SUPELCOPORTCat. No.: 11835-U (packing)Column: 6' x 2mm ID glassOven: 120°CCarrier: nitrogen, 20mL/minInj.: 1µL (1µg/µL conc.)For more information, request Bulletin 856.795-0081Acids0 2 4 86Min123 456871. Pyruvic acid2. Lactic acid3. Oxalacetic acid4. Oxalic acid5. Methyl malonic acid6. Malonic acid7. Fumaric acid8. Succinic acid795-01580 1 2 3 4 5Min121. D-Valine2. L-Valine1212345 761110980 2 864 10 12 14Min1. Dimethyl malonate (C3)2. Dimethyl succinate (C4)3. Dimethyl glutarate (C5)4. Dimethyl adipate (C6)5. Dimethyl pimelate (C7)6. Dimethyl suberate (C8)7. Dimethyl azelate (C9)8. Dimethyl sebacate (C10)9. Dimethyl terephthalate10. Dimethyl isophthalate11. Dimethyl phthalate713-0976795-0159Figure 13. Methyl Esters of Dibasic AcidsPacking: 10% SP-2340 on 100/120 Chromosorb W AWCat. No.: 11852 (packing)Column: 6' x 1/8" ID stainless steelOven: 170°C to 220°Cat 4°C/minCarrier: nitrogen, 20mL/minInj.: 0.75µL, 10mg/cceach acidFigure 14. Bacterial Acid StandardPacking: 3% SP-2100 DOH on 100/120 SUPELCOPORTCat. No.: 12101 (packing)Column: 10' x 2mm ID glassOven: 150°C to 225°C at 4°C/minCarrier: nitrogen, 20mL/minDet.: FIDInj.: 1µL0 2 4 6 8 10 12 14 16 18 20MinFor more information, request Bulletin 856 and Application Note 50.13. 16:014. -17:015. 17:016. 17:017. 2-OH 16:018. 3-OH 16:019. 18:120. 18:021. 19:022. 19:023. 20:01. 11:02. 2-OH 10:03. 12:04. 13:05. 2-OH 12:06. 3-OH 12:07. 14:08. -15:09. 15:010. 2-OH 14:011. 3-OH 14:012. 16:1123 456 7891011121315171816192021222314131. Methyl lithocholate (0.94µg/µL)2. Methyl deoxycholate (1.25µg/µL)3. Methyl chenodeoxycholate (1.57µg/µL)4. Methyl cholate (2.50µg/µL)MinFor more information, request Bulletin 856. 713-097713240 4 8 12 16 201. Acetaldehyde2. Methanol3. Propanol4. Acetone5. Methyl acetate6. Ethanol7. Isobutanol8. Butanal9. Isopropanol10. Ethyl acetate11. Diacetal12. n-Propanol13. Isopentanal14. sec-Butanol15. Pentanal16. Ethyl propionate17. Propyl acetate18. Isobutanol19. Acetal20. Butanol21. Ethyl isobutyrate22. 3-Methylbutan-2-ol23. 3-Pentanol24. 2-Pentanol25. Isobutyl acetate26. Ethyl butyrate27. Butyl acetate28. 2-Methylpentan-1-ol29. 1-Methylpentan-1-ol30. Acetic acid31. Pentanol32. Isoamyl acetate33. Furfural34. Propionic acid35. Hexanol36. Isobutyric acid37. Butyric acid0 2 4 6 8 10 12 14 16 18 26242220Min321456,7891011121315,161418202224,25261721232728293031333736353432Figure 16. Alcoholic Beverage/Fermentation ProductsPacking: 80/120 Carbopack B AW/6.6% PEG 20MCat. No.: 11814 (packing, 15g/bottle)Column: 2m x 2mm ID glassOven: 80°C to 200°C at 4°C/minCarrier: nitrogenDet.: FIDInj.: 1µL water:ethanol (50:50),40-60ppm each analyteUse this packing in glass columns only.For more information, request Bulletin 790.Figure provided by Dr. Antonio DiCorcia and colleagues, University of Rome (used with permission of ElsevierScientific Publishing Co., Amsterdam). 713-0711Acids, Methyl Esters/AlcoholsFigure 15. Bile Acid Methyl EstersPacking: 3% SP-2250 on 100/120 SUPELCOPORTCat. No.: 11878 (packing, 20g/bottle)Column: 3' x 2mm ID glassOven: 275°CCarrier: helium, 40mL/minDet.: FID1914For more information, request Bulletin 790.Chromatogram provided courtesy of Hayes Separations, Inc.For more information, request Bulletin 790.Figure 18. Cherry BrandyPacking: 80/100 Carbopack B AW/5% Carbowax 20MCat. No.: 11812-U (packing)Column: 2m x 2mm ID glass(stock column available)Oven: 70°C to 170°C at 5°C/minCarrier: helium, 20mL/minDet.: FIDInj.: 0.5µL cherry brandy795-008312345U6108U90 2 4 6 8 10 12 14 16Min7Figure 17. Tequila HeadspacePacking: HayeSep D, 80/120 meshCat. No.: 10293 (packing)Column: 3' x 1/8" ID stainless steelOven: 100°CCarrier: helium, 30cc/minDet.: P.E. 900 T.C., 225 ma, 140°CInj.: Valco valve, 100µL, 140°C3 54210Min5642131. Air (Att. x1)2. Carbon dioxide (Att. x1)3. Water (Att. x1)4. Methanol (Att. x1)5. Acetaldehyde (Att. x1)6. Ethanol (Att. x8)1. Acetaldehyde2. Methanol3. Ethanol4. Ethyl acetate5. n-PropanolU Unknown6. Isobutanol7. Acetal8. Acetic acid9. Active amyl alcohol10. Isoamyl alcoholU Unknown795-016015795-0084795-0085Figure 20. Scotch WhiskeyPacking: 80/120 Carbopack B/6.6% Carbowax 20MCat. No.: 11814 (packing)Column: 2m x 2mm ID glassOven: 80°C to 150°C at 4°C/minCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.5µL Scotch whiskeyAlcohols1. Acetaldehyde2. Methanol3. Ethanol4. Ethyl acetate5. n-Propanol6. Isobutanol7. Acetic acid8. Active amyl alcohol9. Isoamyl alcohol123645 7890 42 86 10 12 14 16MinFigure 19. BourbonPacking: 80/120 Carbopack B AW/5% Carbowax 20MCat. No.: 11812-U (packing)Column: 2m x 2mm ID glass(stock column available)Oven: 70°C to 170°C at 5°C/minCarrier: helium, 20mL/minDet.: FIDInj.: 0.5µL bourbon whiskeyFor more information, request Bulletin 790.For more information, request Bulletin 790.2 4 8 14 16Min10 12601245 689731. Acetaldehyde2. Methanol3. Ethanol4. Ethyl acetate5. n-Propanol6. Isobutanol7. Active amyl alcohol8. Isoamyl alcohol9. Acetic acid16For more information, request Bulletin 790.795-0161Figure 21. Denaturants in AlcoholPacking: GP 80/100 Carbopack C/0.1% SP-1000Cat. No.: 11820 (packing)Column: 6' x 2mm ID glass(stock column available)Oven: 80°CCarrier: nitrogen, 20mL/minDet.: FIDInj.: 70% ethanol & 2% ofeach denaturantFigure 22. Blood AlcoholsPacking: 60/80 Carbopack B/5% Carbowax 20MCat. No.: 11766 ((packing, 15g/bottle)Column: 6' x 2mm ID glass(stock column available)Oven: 85°CCarrier: helium, 20mL/minDet.: FIDInj.: 1µL water,0.05-0.10% each analyte1. Methanol2. Ethanol3. Acetone4. Isopropanol5. n-Propanol6. tert-Butanol7. Chloroform, methyl ester8. Methyl Cellosolve®9. sec-Butanol10. Isobutanol11. Ethyl acetate12. n-Butanol13. Ethyl Cellosolve14. Benzene15. Methyl isobutyl ketone0 2Min2345671143650 1 2 3 4 5 6Min21. Acetaldehyde2. Methanol3. Acetone4. Ethanol5. Isopropanol6. n-PropanolFor more information, request Application Note 72.713-06224 6 8 108910111312141522 24170Min2 4 6 8 10 12 14132456789Figure 23. Alcohols (C1-C5) and AcetaldehydePacking: 80/120 Carbopack B AW/5% Carbowax 20MCat. No.: 11812-U (packing, 15g/bottle)Column: 2m x 2mm ID glass(stock column available)Oven: 70°C to 170°C at 5°C/minCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.5µL Scotch whiskeyFigure 24. Isomeric Alcohols (C1-C5)Packing: 80/100 Carbopack C/0.2% Oronite® NIWColumn: 2m x 2mm ID glassOven: 125°CCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.1µLFor more information, request Bulletin 790.795-0162AlcoholsUse this packing in glass columns only.For more information, request Bulletin 790.713-07101. Acetaldehyde2. Methanol3. Ethanol4. Ethyl acetate5. n-Propanol6. Isobutanol7. Acetic acid8. Active amyl alcohol9. Isoamyl alcohol0 2 4 6 108Min123465710111291314161581. Methanol2. Ethanol3. 2-Propanol4. 1-Propanol5. 2-Methyl-2-propanol (tert-butyl)6. 2-Butanol (sec-butyl)7. 2-Methyl-1-propanol (isobutyl)8. 1-Butanol9. 2-Methyl-2-butanol (tert-amyl)10. 2,2-Dimethyl-1-propanol11. 3-Methyl-2-butanol12. 3-Pentanol13. 2-Pentanol14. 2-Methyl-1-butanol (active)15. 3-Methyl-1-butanol16. 1-Pentanol18713-1069Figure 25. Isomeric Alcohols (C1-C5)Packing: 80/100 Carbopack F-SLColumn: 2m x 2mm ID glassOven: 120°CCarrier: nitrogen, 15mL/minDet.: FIDInj.: 0.1µLFigure 26. Alcohols (C10-C18)Packing: 10% SP-2100 on 80/100 SUPELCOPORTCat. No.: 12140 (packing)Column: 6' x 1/8" ID stainless steel(stock column available)Oven: 225°CCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.5µL, 0.1% each in chloroformFor more information, request Bulletin 790.0 21 3 4 5Min123456781516131412111091. Methanol2. Ethanol3. 2-Propanol4. 1-Propanol5. 2-Methyl-2-propanol (tert-butyl)6. 2-Butanol (sec-butyl)7. 2-Methyl-1-propanol (isobutyl)8. 1-Butanol9. 2-Methyl-2-butanol (tert-amyl)10. 2,2-Dimethyl-1-propanol11. 3-Methyl-2-butanol12. 3-Pentanol13. 2-Pentanol14. 2-Methyl-1-butanol (active)15. 3-Methyl-1-butanol16. 1-Pentanol4 5 6 7 98Min5431 1. 1-Decanol (C10)2. 1-Dodecanol (C12)3. 1-Tetradecanol (C14)4. 1-Hexadecanol (C16)5. 1-Octadecanol (C18)0 1 2 32For more information, request Bulletin 790.795-01631019Figure 27. Alcohols (C14:0-C18:0, C18:1, & C18:2)Packing: 10% SP-2300 on 80/100 SUPELCOPORTCat. No.: 12091 (packing)Column: 6' x 1/8" ID stainless steelOven: 200°CCarrier: nitrogen, 20mL/minDet.: FIDInj.: 1µL, 1% total sample in chloroform795-0164795-0165Alcohols/Amines0 2 4 6 10 12 14 16 18 20 228Min2134567Figure 28. Aliphatic Amines in Water (100ppm)Packing: Carbopack B/4% Carbowax 20M/0.8% KOHCat. No.: 11887 (packing)Column: 6' x 2mm ID glass(stock column available)Oven: 90°C to 150°C at 4°C/minCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.5µL1. Methylamine2. Dimethylamine3. Ethylamine4. Trimethylamine5. Isopropylamine6. n-Propylamine7. tert-Butylamine8. Diethylamine9. sec-Butylamine10. Isobutylamine11. n-Butylamine0 1 2 3 4 5 6 7 98Min12345 7689101110For more information, request Bulletin 737.1. C14:02. C14:13. C16:04. C16:15. C18:06. C18:17. C18:220713-0717795-0166For more information, request Bulletin 737.Figure 29. AminesPacking: 80/100 HayeSep BCat. No.: 10286 (packing,75cc/bottle)Column: 5' x 1/8" ID stainless steelOven: 140°C to 190°C at 16°C/minCarrier: helium, 30cc/minDet.: thermal conductivity (175ma, 180°C)Inj.: 0.2µL, on-column injection, 150°C23210 1 3 4 5Min456For more information, request Bulletin 737.Chromatogram provided courtesy of Hayes Separations, Inc.Figure 30. Heterocyclic Amines in Water (1000ppm)Packing: Carbopack B/4% Carbowax 20M/0.8% KOHCat. No.: 11887 (packing)Column: 6' x 2mm ID glass(stock column available)Oven: 140°CCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.4µL1. Air2. Water3. Methylamine4. Dimethylamine5. Trimethylamine6. Ethylene diamine1. Ethylenediamine2. Piperidine3. Pyridine4. Morpholine5. Piperazine6. Cyclohexylamine0 2 4 6 8 10 12 14 16Min15643221Figure 32. TMS Amino AcidsPacking: 10% OV-11 on 100/120 SUPELCOPORTCat. No.: 11961 (packing)Column: 6' x 2mm ID glassOven: 100°C (5 min) to 230°C at 3°C/minCarrier: nitrogen, 30mL/min795-0167For more information, request Bulletin 737.713-0715542IS130 2 4 6 8 10Min11ng Each Amine1. ButylamineIS Triethylamine (int. std.)2. n-Amylamine3. n-Hexylamine4. n-Heptylamine5. n-OctylamineAmines/Amino Acids1. ALA2. GLY23. IMP4. VAL5. LEU6. GLY37. PRO8. SER9. THR10. HYPRO11. ASP12. MET13. GLU214. GLU315. PHE16. ARG17. LYS18. TYR19. HIS20. TRP21. CYS0 8 2416 32 48 5640Min212019181716152131312119857641014Figure 31. AminesPacking: 60/80 Carbopack B/4% Carbowax 20M/0.8% KOHCat. No.: 11887 (packing, 15g/bottle)Column: 2m x 2mm ID glass(stock column available)Oven: 100°C (2 min) to 220°C at 16°C/minCarrier: helium, 30mL/minDet.: thermionic (nitrogen-specific)22Figure 33. AroclorsPacking: 1.5% SP-2250/1.95% SP-2401on 100/120 SUPELCOPORTCat. No.: 11947 (packing)Column: 2m x 1/4" OD x 4mm IDTightSpec glass(stock column available)Oven: 160°C (Aroclor 1016, 1221,1232, 1242, 1248)200°C (Aroclor 1254, 1260)Carrier: nitrogen, 60mL/minDet.: ECDInj.: 5ng each Aroclor in5µL isooctane795-0170For more information, request Bulletin 817and Application Note 67.0 8 16 24 32 40 48 56MinAroclor® 1016Aroclor 1221Aroclor 1232Aroclor 1242Aroclor 1248Aroclor 1254Aroclor 126023Figure 34. AroclorsPacking: 3% SP-2100 on 100/120SUPELCOPORTCat. No.: 11738 (packing)Column: 2m x 1/4" OD x 2mm ID TightSpec glass(stock column available)Oven: 170°C (Aroclor 1016, 1242,1248, 1254, 1260)140°C (Aroclor 1221, 1232)Carrier: nitrogen, 40mL/minDet.: ECDInj.: 3ng each Aroclor in 3µL isooctaneAroclorsFor more information, request Bulletin 817 and Application Note 67.795-0171Aroclor 1254Aroclor 1242Aroclor 1248Aroclor 1232Aroclor 1221Aroclor 1016Aroclor 126032241680Min241. Air2. Ammonia (35%)3. Water (65%)For more information, request Bulletin 786.Figure 36. Aromatics and AliphaticsPacking: 10% TCEP on 100/120 Chromosorb P AWCat. No.: 12106-U (packing, 20g/bottle)Column: 8' x 1/8" ID stainless steelOven: 80°CCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.2µL, approx. equal volumes each analyte713-0724Figure 35. AmmoniaPacking: HayeSep P, 60/80 meshCat. No.: 10297 (packing)Column: 8' x 1/8" ID stainless steelOven: 80°CCarrier: helium, 30cc/minDet.: T.C.(175ma), 200°CInj.: 0.1µL of NH4OH, on-column, 150°C795-01680 1 3Min212 30 2 4 6 8 10 12 14 16 18Min123456789,1011121. n-Hexane2. n-Heptane3. n-Octane4. n-Nonane5. n-Decane6. Benzene7. Toluene8. Ethylbenzene9. m-Xylene10. p-Xylene11. Isopropylbenzene12. o-XyleneFor more information,request Bulletin 743.25For more information, request Bulletin 743.795-0169Figure 38. Aromatic Impurities in EthylbenzenePacking: 80/100 Carbopack C/0.1% SP-1000Cat. No.: 11820 (packing)Column: 6' x 1/8" ID stainless steelCat. No.: 12495-U (general configuration stock column;other stock columns available)Oven: 225°CCarrier: nitrogen, 20mL/min at 42psi711-0097For more information, request Bulletin 743.Figure 37. Aromatics in GasolinePacking: 35% BC-150 on 100/120 Chromosorb P AWCat. No.: 11840-U (packing)Column: 10' x 1/8" ID stainless steelOven: 150°C (5 min) to 200°C at 16°C/minCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.5µL gasolineAmmonia/Aromatics1. BenzeneU Unknown2. Toluene3. Ethylbenzene4. Isopropylbenzene5. XylenesU Unknown0 2 4 6 8 10 12Min141. C1-C12 aliphatic hydrocarbons2. Benzene3. Toluene1230 1 2 43 5 6 7 8Min1U 234 5U26For more information, request Bulletin 743.711-0094For more information, request Bulletin 743.711-0096Figure 39. AromaticsPacking: 80/100 Carbopack C/0.1% SP-1000Cat. No.: 11820 (packing)Column: 6' x 1/8" ID stainless steelCat. No.: 12495-U (general configuration stock column;other stock columns available)Oven: 200°CCarrier: nitrogen, 20mL/min at 42psi0 1 2 3 4 6 75 98Min124357861. Benzene2. n-Hexane3. Toluene4. Ethylbenzene5. Isopropylbenzene6. m- & p-Xylene7. o-Xylene8. n-PropylbenzeneFigure 40. AromaticsPacking: 5.0% SP-1200/1.75% Bentone® 34 on 100/120 SUPELCOPORTCat. No.: 12134 (packing)Column: 6' x 1/8" ID stainless steelCat. No.: 12721 (general configuration stock column;other stock columns available)Oven: 75°CCarrier: nitrogen, 20mL/minInj.: 0.1µL0 2 4 6 8 10 12 14 16Min1851 2 3467891. Benzene2. Toluene3. Ethylbenzene4. p-Xylene5. m-Xylene6. o-Xylene7. Isopropylbenzene8. Styrene9. n-Propylbenzene27For more information, request Bulletin 743.713-0721Figure 42. ButylbenzenesPacking: 60/80 Carbopack F-TACat. No.: packing available in packed columns onlyColumn: 2m x 2mm ID glassOven: 225°CCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.05µL methyl ethyl ketone(5% each analyte)Figure 41. ButylbenzenesPacking: 80/100 Carbopack C/0.1% SP-1000Cat. No.: 11820 (packing, 15g/bottle)Column: 6' x 1/8" ID stainless steelCat. No.: 12495-U (general configuration stock column,other stock columns available)Oven: 225°CCarrier: nitrogen, 20mL/minDet.: FIDInj.: 1µL chloroform(~0.1% each analyte)1. tert-Butylbenzene2. sec-Butylbenzene3. Isobutylbenzene4. n-Butylbenzene20 4 6Min4321For more information, request Bulletin 743.713-0720Aromatics0 2 4 6 8 10Min12341. tert-Butylbenzene2. sec-Butylbenzene3. Isobutylbenzene4. n-Butylbenzene288711-0100For more information, request Bulletin 743.Figure 43. Tri- and TetramethylbenzenesPacking: 10% SP-2250 on 100/120 SUPELCOPORTCat. No.: 12132 (packing)Column: 10' x 1/8" ID stainless steelOven: 110°CCarrier: nitrogen, 20mL/minInj.: 0.6µL, solution 0.1% each component in CHCl3Figure 44. Vinyl ChloridePacking: 80/100 Carbopack C/0.19% picric acidCat. No.: 11824 (packing)Column: 6' x 1/8" ID stainless steelCat. No.: 13867 (general configuration stock column only)Oven: 50°CCarrier: nitrogen, 40mL/minDet.: FID, Varian 3700Inj.: 1µL, 1ppm vinyl chloride in carbon disulfide1. Vinyl chloride (1ppm)2. Carbon disulfideFor more information, request Bulletin 846.795-01726 8 1042 120 14 16 24 26 282220 3018Min1235 641. 1,3,5-Trimethylbenzene2. 1,2,4-Trimethylbenzene3. 1,2,3-Trimethylbenzene4. 1,2,4,5-Tetramethylbenzene5. 1,2,3,5-Tetramethylbenzene6. 1,2,3,4-Tetramethylbenzene0 1 2 3 4 5 6 7 9Min1229Figure 45. Vinyl ChloridePacking: 60/80 Carbopack C/0.2% Carbowax 1500Cat. No.: 11826 (packing)Column: 6' x 1/8" ID stainless steelCat. No.: 13860-U (general configuration stock column;other stock columns available)Oven: room temperatureCarrier: nitrogen, 20mL/minDet.: FIDInj.: 1mL nitrogen containing 1ppm VC0 2 4 6 81 23Blank4795-0173Min713-0694,0695For more information, request Bulletins 846 and 861.Chlorinated HydrocarbonsFigure 46. Methylene Chloride andVinyl Chloride by OSHA Method 51Sample: Carboxen™-564 spiked with vinylacetate (18ng) and desorbed withmethanol:methylene chloride (5:95)Sampling Tube: ORBO™-92Cat. No.: 20362Packing: 80/100 Carbopack C/0.2% Carbowax 1500Cat. No.: 11827 (packing, 15g/bottle)Column: 6' x 2mm ID glassOven: 90°CCarrier: nitrogen, 20mL/minDet.: FIDInj.: 1µLFor more information, requestBulletins 846, and 866.0 1 2 3010204030Min1. Methanol2. Methylene chloride3. Solvent impurity4. Vinyl acetateStandard representing0.75mg/m3 vinyl acetatein 24L air sample30Figure 48. Volatile Compounds byUS EPA Methods TO-2 and TO-3Sample: 1-5ng each componentDesorption Tube: Carbotrap 300, 4mm IDCat. No.: 20379Carrier: helium, 20mL/minPacking: 60/80 Carbopack B/1% SP-1000Cat. No.: 11815 (packing, 15g/bottle)Column: 8' x 1/8" stainless steelCat. No.: 12543 (general configuration stockcolumn; other stock columns available)Oven: 35°C (2 min) to 220°C at 16°C/minDet.: FIDFigure 47. Chlorinated Volatile Hydrocarbons byUS EPA Method TO-1Sample: 2ng each componentDesorption Tube: Carbotrap™ 300, 4mm IDCat. No.: 20379Thermal Desorption: 330°C, 4 minTransfer Line Temp.: 220°CCarrier (desorptiontube to column): helium, 20mL/minPacking: 80/100 Carbopack C/0.1% SP-1000Cat. No.: 11820 (packing, 15g/bottle)Column: 6' x 1/8" stainless steelCat. No.: 12495-U (general configuration stock column;other stock columns available)Oven: 35°C (2 min) to 220°C at 16°C/minCarrier: helium, 20mL/minDet.: FIDFor more information, request Bulletins 846 and 861. 713-0702For more information, request Bulletins 846 and 861.713-07090 84 12 16 20Min1234,51. Vinyl chloride2. Dichloromethane3. Acrylonitrile4. 1,1-Dichloroethylene5. 3-Chloropropene6. Chloroform7. 1,2-Dichloroethane8. 1,1,1-Trichloroethane9. Carbon tetrachloride10. Benzene11. Tetrachloroethylene12. Toluene13. Chlorobenzene6789101112130 4 82 6Min1412103245678911101. Methanol (carrier solvent)2. Chloroform3. 1,2-Dichloropropane4. 1,1,1-Trichloroethane5. Carbon tetrachloride6. 1,2-Dichloroethane7. Trichloroethylene8. Bromoform9. Tetrachloroethylene10. Impurity11. Chlorobenzene131Figure 50. CreosotePacking: 10% SP-2100 on 100/120 SUPELCOPORTCat. No.: 11989 (packing)Column: 10' x 1/8" ID stainless steelCat. No.: 12717 (general configuration stock column;other stock columns available)Oven: 100°C to 300°C at 6°C/minCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.1µL0 4 12 168 20 24 28 32 36 40Min12345678 9101113141512For more information, request Bulletin 743 and Application Note 108.713-07491. Naphthalene2. 2-Methylnaphthalene3. 1-Methylnaphthalene4. Biphenyl5. 2,6-Dimethylnaphthalene6. 2,3-Dimethylnaphthalene7. Acenaphthalene8. Dibenzofuran9. Fluorene10. Methyl fluorenes11. Phenanthrene & Anthracene12. Carbazole13. Fluoranthene14. Pyrene15. Chrysene795-0174CreosoteMin0 2 4 6 81 3 115 7 9 10111 23456791081. Methylene chloride2. 1,1-Dichloroethane3. Chloroform4. 1,2-Dichloroethane5. 1,1,1-Trichloroethane6. Carbon tetrachloride7. Trichloroethylene8. 1,1,2-Trichloroethane9. Perchloroethylene10. 1,1,1,2-Tetrachloroethane11. 1,1,2,2-TetrachloroethaneFor more information,request Bulletin 824.Figure 49. Chlorinated SolventsPacking: 20% SP-2100/0.1% Carbowax 1500 on 100/120 SUPELCOPORTCat. No.: 11821 (packing)Column: 10' x 1/8" ID stainless steelCat. No.: 12718-U (general configuration stock column;other stock columns available)Oven: 100°CCarrier: nitrogen, 20mL/minDet.: FID32For more information, request Bulletin 743 and Application Note 108.711-0099For more information, request Bulletin 743.795-0175Figure 52. Creosote with Liquid Crystal PhasePacking: 1.5% SP-2100/1% BMBT on 100/120 SUPELCOPORTCat. No.: customColumn: 10' x 1/8" ID stainless steelOven: 85°C to 225°C at 6°C/minCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.1µL creosoteFigure 51. CreosotePacking: 10% SP-2250 on 100/120 SUPELCOPORTCat. No.: 12132 (packing)Column: 10' x 1/8" ID stainless steelOven: 100°C to 300°C at 6°C/minCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.1µL1. Naphthalene2. 2-Methylnaphthalene3. 1-Methylnaphthalene4. Biphenyl5. 2,6-Dimethylnaphthalene6. 2,3-Dimethylnaphthalene7. Acenaphthalene8. Dibenzofuran9. Fluorene10. Methyl fluorenes11. Phenanthrene & Anthracene12. Carbazole13. Fluoranthene14. Pyrene0 4 8 12 16 20 24 28 32 36 40 44 48 52 56 60Min12346789111213141051. Naphthalene2. 2-Methylnaphthalene3. 1-Methylnaphthalene4. Biphenyl5. 2,6-Dimethylnaphthalene6. 2,3-Dimethylnaphthalene7. Acenaphthalene8. Dibenzofuran9. Fluorene10. Methyl fluorenes11. Phenanthrene12. Anthracene13. Carbazole14. Fluoranthene15. Pyrene0 4 12 24 28Min8 32201612354768910121113 14 153 3Figure 54. NicotinePacking: 10% Carbowax 20M/2% KOHon 80/100 Chromosorb W AWCat. No.: 11805 (packing)Column: 6' x 2mm IDOven: 200°CCarrier: 20mL/minInj.: 1µLFigure 53. Cannibinoids as TMS DerivativesPacking: 3% SP-2250 on 100/120 SUPELCOPORTCat. No.: 11875 (packing)Column: 6' x 2mm ID glassOven: 235°CCarrier: nitrogen, 20mL/minDet.: FID, 275°C1. Cannabidiol2. Cannabichromene3. Cannabigerol4. 6-THC5. 1-THC6. Cannabinol795-0176Creosote/DrugsFor more information, request Bulletin 737.795-01770 2 4 6 108Min53216Nicotine0 1 2 3 4Min43 4Figure 55. Anticonvulsant Drugs, UnderivatizedPacking: 2% SP-2110/1% SP-2510-DA on 100/120 SUPELCOPORTCat. No.: 11768 (packing, 20g/bottle)Column: 3' x 2mm ID glassOven: 120°C to 250°C at 16°C/min, hold 4 minCarrier: helium, 50mL/minDet.: FIDInj.: 2µL, analyte amounts shown on figureFigure 56. Anticonvulsant Drugs, UnderivatizedPacking: 2% SP-2510-DA on 100/120 SUPELCOPORTCat. No.: 11776 (packing, 20g/bottle)Column: 3' x 2mm ID glassOven: 150°C to 265°C at 16°C/min,hold 4 minCarrier: nitrogen, 50mL/minDet.: FIDInj.: 2µL1. Ethosuximide2. Methsuximide3. Phensuximide4. Barbital (int. std.)5. N-Desmethyl methsuximide6. Mephenytoin7. N-Desmethyl phensuximide8. Phenylethylmalonamide9. 5-Methyl-5-phenythydantoin (int. std.)10. Phenobarbital11. Carbamazepine12. Primidone13. Diphenythydantoin14. 5-p-Methylphenyl-5-phenythydantoin(int. std.)713-0626234 567189101112 13140 1 2 3 4 5 6 7 8 9Min11102 4 6 8 10 1234651178910120Min1. Ethosuximide, 1.0µg2. -Methyl, -propyl succinimide (int. std.), 1.0µg3. Phenylethylmalonamide (PEMA), 0.2µg4. p-Methyl PEMA (int. std.), 0.2µg5. Phenobarbital, 0.5µg6. p-Methyl phenobarbital (int. std.), 0.5µg7. Carbamazepine, 0.2µg8. Promidone, 0.2µg9. p-Methyl primidone (int. std.), 0.2µg10. Phenytoin, 0.3µg11. p-Methyl phenytoin (int. std.), 0.5µg713-06253 5Figure 58. Psychotropic Drugs, Antidepressants,UnderivatizedPacking: 3% SP-2250 on 80/100 SUPELCOPORTCat. No.: 11767 (packing, 20g/bottle)Column: 6' x 2mm ID glassOven: 250°CCarrier: helium, 40mL/minDet.: NPDInj.: 5µL methanol,10ng each drugFigure 57. Anticonvulsant DrugsPacking: 10% SP-1000 on 80/100 SUPELCOPORTCat. No.: 11872 (packing)Column: 3' x 2mm ID glassOven: 190°CCarrier: helium, 40mL/minDet.: FID, 250°CInj.: 0.25µg/mL each drug713-0675713-067613420 1 2 43 5Min1. Valproic acid2. Cyclohexane carboxylic acid3. , -Dimethyl- -methylsuccinimide4. Ethosuximide1. Amitriptyline2. Nortriptyline3. Clomipramine1230 2 4 6MinDrugs3 6Figure 59. BarbituratesPacking: 3% SP-2250 DA on 100/120 SUPELCOPORTCat. No.: 11981 (packing)Column: 6' x 2mm ID glassOven: 230°CCarrier: nitrogen, 20mL/minInj.: 1µL, 0.1µg/µL each component1. Amobarbital2. Pentobarbital3. Secobarbital4. PhenobarbitalFigure 60. AlkaloidsPacking: 3% SP-2250 DB on 100/120 SUPELCOPORTCat. No.: 11983 (packing, 20g/bottle)Column: 3' x 2mm ID glassOven: 230°CCarrier: nitrogen, 40mL/minDet.: FIDInj.: 1µL methanol, analyte quantities shown on figure713-0664795-01780 1 2 43Min43121. Procaine, 0.2µg2. Methadone, 0.2µg3. Cocaine, 0.4µg4. Codeine, 0.4µg5. Heroin, 0.4µg6. Quinine, 2.0µg1234560 2 3 4 5 6 7 8 9 10Min3 7Figure 61. US EPA Method 601, Purgeable HalocarbonsPacking: 60/80 Carbopack B/1% SP-1000Cat. No.: 11815 (packing)Column: 8' x 1/8" OD stainless steelCat. No.: 12543 (general configuration stock column; other stock columns available)Oven: 45°C (3 min) to 220°C at 8°C/min, 15 min holdCarrier: nitrogen, 40mL/minDet.: Hall®, 85°CInj.: 1µL of a synthetic mixture of volatile pollutants in dodecane, 200-500ng/µLeach component, 200°CEPA Methods (600 Series)0 2 4 6 108 12 14 16 18 20 22MinFor more information, requestBulletins 775 and 865.795-01791 24357IS68910111314IS171920IS1812 151621For more information, requestBulletins 775 and 865.795-01801. Chloromethane2. Bromomethane3. Vinyl chloride & Dichlorofluoromethane4. Chloroethane5. Methylene chloride6. Trichlorofluoromethane7. 1,1-DichloroethyleneIS Bromochloromethane (int. std.)8. 1,1-Dichloroethane9. trans-1,2-Dichloroethylene10. Chloroform11. 1,2-Dichloroethane12. 1,1,1-Trichloroethane13. Carbon tetrachloride14. Bromodichloromethane15. 1,2-Dichloropropane &trans-1,3-Dichloropropene16. Trichloroethylene17. cis-1,3-Dichloropropene, 1,1,2-Trichloroethane, &ChlorodibromomethaneIS 1-Chloro-2-bromopropane (int. std.)18. 2-Chloroethyl vinyl ether19. Bromoform20. 1,1,2,2-Tetrachloroethylene & 1,1,2,2-Tetrachloro-ethaneIS 1,4-Dichlorobutane (int. std.)21. Chlorobenzene0Min2 4 6 8 10 12 14 16 18 20 221. Benzene2. Toluene3. Ethylbenzene4. Chlorobenzene5. 1,4-Dichlorobenzene6. 1,3-Dichlorobenzene7. 1,2-Dichlorobenzene132 45 67Figure 62. US EPA Method 602, Purgeable AromaticsPacking: 5% SP-1200/1.75% Bentone 34 on 100/120 SUPELCOPORTCat. No.: 12134 (packing)Column: 6' x 1/8" ID stainless steelCat. No.: 12721 (general configuration stock column; other stock columns available)Oven: 50°C (2 min) to 90°C at 6°C/min and holdCarrier: helium, 36mL/minDet.: FIDInj.: 1µL of 0.2mg/mL standard of each purgeable aromatic in methanol3 8Figure 64. US EPA Method 604, Derivatives of PhenolsPacking: 5% SP-2250 on 80/100 SUPELCOPORTCat. No.: 11737 (packing)Column: 1.8m x 2mm ID glassOven: 160°C (2 min) to 240°C at 8°C/minCarrier: nitrogen, 30mL/minDet.: ECInj.: 0.1µL with 0.2µg/mL of each component in methanolFigure 63. US EPA Method 603, Acrolein and AcrylonitrilePacking: 80/100 Chromosorb 101Cat. No.: 20214 (packing)Column: 6' x 1/8" OD stainless steelCat. No.: 12712 (general configuration stock column; other stock columns available)Oven: 100°C (5 min) to 140°C at 10°C/min and holdCarrier: helium, 45mL/minDet.: FIDInj.: 2µL synthetic standard in waterFor more information, request Bulletins 775 and 865.795-01811. Acrolein, 3mg/mL2. Acrylonitrile, 1mg/mL0 2 4 6 8 10 12Min12For more information, request Bulletins 775 and 865.795-01820 2 4 6 8 10 12MIn213456 7891. Phenol2. 2,4-Dimethylphenol3. 2-Chlorophenol4. 4-Methyl-3-chlorophenol5. 2,4-Dichlorophenol6. 2,4,6-Trichlorophenol7. 2-Nitrophenol8. 4-Nitrophenol9. Pentachlorophenol3 9Figure 65. US EPA Method 604, PhenolsPacking: 1% SP-1240-DA on 100/120 SUPELCOPORTCat. No.: 11832 (packing, 20g/bottle)Column: 2m x 2mm ID glassOven: 70°C (2 min) to 200°C at 8°C/min and holdCarrier: helium, 30mL/minDet.: FID, 250°CInj.: 0.5µL EPA Phenols Mix (Cat. No. 48859)For more information, request Bulletins 775 and 865.795-0183Figure 66. US EPA Method 606, PhthalatesPacking: 1.5% SP-2250/1.95% SP-2401 on 100/120 SUPELCOPORTCat. No.: 11947 (packing)Column: 2m x 4mm ID glass (stock column available)Oven: 180°C and 220°CCarrier: nitrogen, 60mL/minDet.: ECD, 250°CInj.: 1µL of phthalate mixture at concentrations indicatedFor more information, request Bulletins 775 and 865.713-10321. Methanol2. o-Chlorophenol (0.25µg)3. 2-Nitrophenol (0.25µg)4. Phenol (0.25µg)5. 2,4-Dimethylphenol (0.25µg)6. 2,4-Dichlorophenol (0.25µg)7. 2,4,6-Trichlorophenol (0.75µg)8. 4-Chloro-m-cresol (1.25µg)9. 2,4-Dinitrophenol (0.75µg)10. 4,6-Dinitro-p-cresol (1.25µg)11. Pentachlorophenol (1.25µg)12. Nitrophenol (1.25µg)0 2 4 6 8 10 12 14 16 18 20 22 24Min1324 56789101112EPA Methods (600 Series)1. Dimethyl phthalate (1.0ppm)2. Diethyl phthalate (0.2ppm)3. Di-n-butyl phthalate (1.0ppm)4. Butyl benzyl phthalate (1.0ppm)5. bis(2-Ethylhexyl) phthalate (0.4ppm)6. Di-n-octyl phthalate (1.0ppm)0 8642 0 2 64 8 10 12 14 16 2018Min180°C 220°C12 3546MIn4 0Figure 67. PhthalatesPacking: 5% SP-2100 on 100/120 SUPELCOPORTCat. No.: 11782-U (packing)Column: 6' x 1/8" stainless steelOven: 200°C (10 min) to 250°C at 4°C/minCarrier: nitrogen, 25mL/minDet.: FIDInj.: 1µL of 75ng/µL each component in carbon disulfideFigure 68. US EPA Method 607, NitrosaminesPacking: 10% Carbowax 20M/2% KOH on 80/100 Chromosorb W AWCat. No.: 11739 (packing)Column: 2m x 4mm ID glassOven: 110°C and 220°CCarrier: helium, 40mL/minDet.: FIDInj.: 2µL of 0.2mg/mL standard in methanol at concentrations indicatedFor more information, request Bulletins 775 and 865.795-01840 2 4 6 8 10 12 14 16Min12SS Carbon disulfide (solvent)1. Di-n-butyl phthalate2. bis(2-Ethylhexyl)phthalate795-0185200 4 8 121230 1616 24 20Min Min110°C 220°C1. N-Nitrosodimethylamine (0.2mg/mL)2. N-Nitroso-di-n-propylamine (0.2mg/mL)3. N-Nitrosodiphenylamine(as Diphenylamine) (0.4mg/mL)4 8 12 24For more information, requestBulletins 775 and 865.4 1Figure 69. N-NitrosaminesPacking: 10% Carbowax 20M/2% KOH on80/100 Chromosorb W AWCat. No.: 11739 (packing)Column: 2m x 4mm ID TightSpec glassOven: 150°CCarrier: helium, 40mL/minDet.: Hewlett-Packard NPDInj.: 1ng each componetnt in2µL methylene chlorideFigure 70. US EPA Method 608, PesticidesPacking: 1.5% SP-2250/1.95% SP-2401 on 100/120 SUPELCOPORTCat. No.: 11947 (packing)Column: 2m x 4mm ID glass(stock column available)Oven: 200°CCarrier: argon:methane(95:5), 6mL/minDet.: EC Ni63Inj.: 0.5µL of 0.02mg/mLof each pesticideFor more information, request Bulletin 775.795-0187For more information, request Bulletin 775.795-0186EPA Methods (600 Series)1. N-Nitrosodimethylamine2. N-Nitrosodi-n-propylamine120 4 6 8 102Min1. -BHC2. -BHC3. -BHC4. Heptachlor5. -BHC6. Aldrin7. Heptachlorepoxide8. Endosulfan I9. 4,4'-DDE10. Dieldrin11. Endrin12. 4,4'-DDD & Endosulfan II13. 4,4'-DDT14. Endrin aldehyde15. Endosulfan sulfate18Min16121086420 141246735891011 121315144 2Figure 72. US EPA Method 610,Polynuclear Aromatic HydrocarbonsPacking: 3% SP-2250 on 100/120 SUPELCOPORTCat. No.: 11744 (packing)Column: 2m x 2mm ID glassOven: 100°C (4 min) to 280°C at 8°C/min and holdCarrier: nitrogen, 40mL/minDet.: FIDInj.: 0.2µg each component in 1µL methanol/methylene chlorideFigure 71. US EPA Method 609,Nitroaromatics and IsophoronePacking: 1.5% SP-2250/1.95% SP-2401 on 100/120 SUPELCOPORTCat. No.: 11947 (packing)Column: 2m x 4mm ID glass(stock column available)Oven: 85°C and 145°CCarrier: nitrogen, 44mL/minDet.: FID for nitrobenzene &isophoroneEC for dinitrotoluenesInj.: 2µL of 0.2mg/mLstandard on FID0.5µL of 2ng/µLstandard on EC795-0188For more information, request Bulletin 775.85°C 145°C1. Nitrobenzene2. Isophorone3. 2,6-Dinitrotoluene4. 2,4-Dinitrotoluene0 2 64 8 0 2 4 6 8 10MinMin12341. Naphthalene2. Acenaphthylene3. Acenaphthene4. Fluorene5. Phenanthrene & Anthracene6. Fluoranthene7. Pyrene8. Benzo(a)anthracene9. Chrysene10. Benzo(b)fluoranthene & Benzo(k)fluoranthene11. Benzo(a)pyrene12. Dibenz(a,h)anthracene & Indeno(1,2,3-cd)pyrene13. Benzo(ghi)perylene795-0189For more information,request Bulletin 775.0 4 8 12 16 20 24 28 32 36 40 4844Min123 457611121381094 3Figure 74. US EPA Method 612, Chlorinated HydrocarbonsPacking: 1.5% OV-1/1.5% OV-225 on 80/100 SUPELCOPORTCat. No.: custom (packing)Column: 2m x 2mm ID glassOven: 75°C and 160°CCarrier: argon:methane (95:5), 30mL/minDet.: ECInj.: 2µL of synthetic mixture (0.1 to 10ng/µL) in hexane1. bis(2-Chloroisopropyl)ether2. 4-Chlorophenyl phenyl ether3. bis(2-Chloroethoxy)methane4. bis(2-Chloroethyl)ether5. 4-Bromophenyl phenyl etherFigure 73. US EPA Method 611, HaloethersPacking: 3% SP-1000 on 100/120 SUPELCOPORTCat. No.: 11746 (packing)Column: 2m x 2mm ID glassOven: 60°C (2 min) to 230°C at 8°C/min and holdCarrier: helium, 40mL/minDet.: FIDInj.: 1µL of 0.2mg/mL of each haloether in methanolEPA Methods (600 Series)For more information, request Bulletins 775 and 865.795-01900 2 4 6 8 10 12 0 4 62 8Min MinFor more information, requestBulletins 775 and 865.795-019175°C 160°C1435269871. 1,3-Dichlorobenzene2. 1,4-Dichlorobenzene3. Hexachloroethane4. 1,2-Dichlorobenzene5. Hexachlorobutadiene6. 1,2,4-Trichlorocyclopentadiene7. Hexachlorocyclopentadiene8. 2-Chloronaphthalene9. Hexachlorobenzene12Min20 4 6 8 10 1814 16 20 22532144 4713-1037Figure 75. US EPA Method 625, Base/NeutralsPacking: 3% SP-2250 on 100/120 SUPELCOPORTCat. No.: 11756 (packing)Column: 2m x 2mm ID glassOven: 50°C (4 min) to 270°C at 8°C/minCarrier: nitrogen, 30mL/minDet.: FID, 300°CInj.: 1µL methylene chloride, 0.125µgor 0.063µg (*) each analyte1. 1,3-Dichlorobenzene2. 1,4-Dichlorobenzene-d43. Bis(2-chloroethyl)ether4. Hexachloroethane & 1,2-Dichlorobenzene5. Bis(methyl-2-chloroethyl)ether6. N-Nitroso-di-n-propylamine7. Nitrobenzene & Hexachlorobutadiene8. 1,2,4-Trichlorobenzene & Isophorone9. Naphthalene & Bis(2-chloroethoxy)methane10. Hexachlorocyclopentadiene11. 2-Chloronaphthalene12. Acenaphthylene13. Acenaphthene14. Dimethylphthalate15. 2,6-Dinitrotoluene16. Fluorene & 4-Chlorophenylphenyl ether17. 2,4-Dinitrotoluene18. Diethylphthalate & N-Nitrosodiphenylamine19. 4-Bromophenylphenyl ether &Hexachlorobenzene20. Phenanthrene & Anthracene21. Di-n-butyl phthalate22. Fluoranthene*23. Pyrene*24. Benzidine25. Butyl benzyl phthalate26. Bis(2-ethylhexyl)phthalate27. Benzo(a)fluoranthene*28. Chrysene*29. 3,3'-Dichlorobenzidine30. Di-n-octyl phthalate31. Benzo(b)fluoranthene* & Benzo(k)fluoranthene*32. Benzo(a)pyrene33. Indeno(1,2,3-cd)pyrene* &Dibenzo(a,h)anthracene*34. Benzo(ghi)perylene*34567 891011131214 15161718192021272322242526 2830293133 34322112356784109111213 141516171918 2021 222324252627 28313230290 4 8 12 16 20 24 28 32 36 40 44MinFigure 76. US EPA Method 624, VolatilesPacking: 60/80 Carbopack B/1% SP-1000Cat. No.: 11815 (packing, 15g/bottle)Column: 8' x 1/8" stainless steelCat. No.: 12543 (general configuration stock column; other stock columns available)Oven: 45°C (3 min) to 220°C at 8°C/min (hold 15 min)Carrier: helium, 40mL/minDet.: FID, 250°CInj.: 1µL of a synthetic mixture of volatilepollutants in dodecane,200-500ng/µLeach, 200°CFor more information, requestBulletins 775 and 865.713-07040 4 8 12 16 20 24 28 32 36 52484440MinFor more information, requestBulletins 775 and 865.1. Chloromethane2. Methanol3. Bromomethane4. Vinyl chloride,Dichlorofluoromethane5. Chloroethane6. Methylene chloride7. Trichlorofluoromethane8. 1,1-Dichloroethylene9. Bromochloromethane (IS)10. 1,1-Dichloroethane11. trans-1,2-Dichloroethylene12. Chloroform13. 1,2-Dichloroethane14. 1,1,1-Trichloroethane15. Carbon tetrachloride16. Bromodichloromethane17. 1,2-Dichloropropane18. trans-1,3-Dichloropropene19. Trichloroethylene20. Benzene21. cis-1,3-Dichloropropene,1,1,2-Trichloroethane,Chlorodibromomethane22. 1-Chloro-2-bromopropene (IS)23. 2-Chloroethyl vinyl ether24. Bromoform25. 1,1,2,2-Tetrachloroethylene,1,1,2,2-Tetrachloroethane26. 1,4-Dichlorobutane (IS)27. Toluene28. Chlorobenzene29. Ethylbenzene30. 1,3-Dichlorobenzene31. 1,2-Dichlorobenzene32. 1,4-Dichlorobenzene4 5For more information, request Bulletins 775 and 865.795-01941. Methanol2. o-Chlorophenol, 0.5mg/mL3. 2-Nitrophenol, 0.5mg/mL4. Phenol, 0.5mg/mL5. 2,4-Dimethylphenol, 0.5mg/mL6. 2,4-Dichlorophenol, 0.5mg/mL7. 2,4,6-Trichlorophenol, 1.5mg/mL8. 4-Chloro-m-cresol, 2.5mg/mL9. 2,4-Dinitrophenol, 1.5mg/mL10. 4,6-Dinitro-o-cresol, 2.5mg/mL11. Pentachlorophenol, 2.5mg/mL12. 4-Nitrophenol, 2.5mg/mLFigure 77. US EPA Method 625, PhenolsPacking: 1% SP-1240-DA on 100/120 SUPELCOPORTCat. No.: 11832 (packing)Column: 2m x 2mm ID glassOven: 70°C (2 min) to 200°C at 8°C/minCarrier: helium, 30mL/minDet.: FIDInj.: 0.5µL of a standard phenol mixture (Cat. No. 48859)Figure 78. US EPA Method 625, PesticidesPacking: 3% SP-2250 on 100/120 SUPELCOPORTCat. No.: 11878 (packing)Column: 2m x 2mm ID glassOven: 50°C (4 min) to 270°C at 8°C/minCarrier: nitrogen, 30mL/minDet.: FIDInj.: 0.1µL of a synthetic mixture of pesticides (1ng/µL each)EPA Methods (600 Series)1. -BHC2. -BHC3. -BHC4. Heptachlor5. -BHC6. Aldrin7. Heptachlorepoxide8. Endosulfan I9. Dieldrin & 4,4'-DDE10. Endrin11. Endosulfan II & 4,4'-DDD12. 4,4'-DDT13. Endosulfan sulfate & Endrin aldehyde0 2 4 6 8 10 12 14 16 18 20 2422Min12111098765432120 6 108 1412 18 22 2624 28 302016 3432Min11213111076245389For more information, request Bulletins 775 and 865.795-019344 6For more information, request Application Note 109.713-0732Figure 79. Ethylene Oxide and Ethylene Oxide ResiduesPacking: 80/100 Carbopack C/0.8% THEEDCat. No.: 11880-U (packing)Column: 1m x 2mm ID glass(stock column available)Oven: 115°CCarrier: nitrogen, 20mL/minDet.: FIDInj.: 1µL water containing50ppm each component1. Ethylene oxide2. Water3. Ethylene chlorohydrin4. Ethylene glycol20 1 2 3 4 5 6Min431. Ethylene oxide, 23ppmFor more information, request Application Note 109.795-0195Figure 80. Trace Ethylene Oxide in NitrogenPacking: HayeSep DCat. No.: 10292 (packing)Column: 10' x 1/8" stainless steelOven: 130°CCarrier: helium, 30mL/minDet.: FID, 140°CInj.: 250mL Valco valve, 100°C15 4 3 2 1 0Min14 7Figure 81. Ethylene Oxide by US Army MethodSample: HBr-coated Carboxen-564 spikedwith 2-bromoethanol (1.2ng) anddesorbed with toluene:acetonitrile (50:50)Sampling Tube: ORBO-78Cat. No.: 20355Packing: 10% DEGS on 80/100 SUPELCOPORTCat. No.: 11999 (packing, 20g/bottle)Column: 12' x 1/8" stainless steelOven: 155°CCarrier: nitrogen, 30mL/minDet.: ECDFigure 82. Fatty Acid Methyl Esters of Rapeseed Oil MixturePacking: 5% DEGS-PS on 100/120 SUPELCOPORTCat. No.: 11870-U (packing, 20g/bottle)Column: 6' x 1/8" stainless steelOven: 200°CCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.5µL RM-3 Mix (Cat. No. O7256-1AMP), 10µg FAMEs/µL in chloroformFor more information,request Bulletin 856.713-09731. C14:02. C16:03. C18:04. C18:15. C18:26. C20:07. C18:38. C22:09. C22:110. C24:00 1 2 3 4 5 6 7 8 9Min1234567 8910Ethylene Oxide/Fatty AcidsS SolventI Solvent impurities2-Bromoethanol0 2 4MinIS2-Bromoethanol back-ground is negligibleBlankEtO AnalysesIIIFor more information, request Application Note 109.713-0696, 06974 8Figure 83. Fatty Acid Methyl Esters of Rapeseed Oil MixturePacking: GP 10% DEGS-PS on 80/100 SUPELCOPORTCat. No.: 11999 (packing, 20g/bottle)Column: 6' x 1/8" stainless steelOven: 200°CCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.5µL RM-3 Mix (Cat. No. O7256-1AMP), 10µg FAMEs/µL in chloroformFigure 84. C18:3, C20:0, and C20:1 Methyl EstersPacking: GP 3% SP-2310/2% SP-2300 on 100/120 Chromosorb W AWCat. No.: 11833 (packing)Column: 6' x 1/8" stainless steelOven: 190°C (2 min) to 220°C at 2°C/minCarrier: nitrogen, 20mL/minDet.: FIDInj.: 5µg mixed esters in 0.5µL methylene chloride1. C14:02. C16:03. C18:04. C18:15. C18:26. C20:07. C18:38. C22:09. C22:110. C24:0For more information, requestBulletin 856.713-10720 2 4 6 8 10 12 14 16 18MIn12345768910For more information, request Bulletin 856.795-0260Min0 2 4 6 8 10 12 14 16 18 201324 5678910111. C14:02. C16:03. C18:04. C18:15. C18:26. C18:37. C20:08. C20:19. C22:010. C22:111. C24:049For more information,request Bulletin 856.795-0197Figure 85. Methyl Elaidate and Oleate FAMEsPacking: 15% OV-275 on 100/120 Chromosorb P AW DMCSCat. No.: 11844-U (packing, 20g/bottle)Column: 20' x 1/8" stainless steelOven: 220°CCarrier: nitrogen, 10mL/minDet.: FIDInj.: 0.5µL isooctane containing 5µg each analyteFigure 86. Whole Milk Free AcidsPacking: 10% SP-216-PS on 100/120 SUPELCOPORTCat. No.: 11879 (packing)Column: 3' x 2mm ID glassOven: 130°C to 200°C at 15°C/minCarrier: nitrogen, 20mL/min1. Methyl elaidate2. Methyl oleateFor more information, request Bulletin 856.713-09740 4 8 12 16 20 24 28 32 36 40Min1 20 1 2 3 4 5 6 7 8 9 10 11Min1. C4:02. C6:03. C8:04. C10:05. C12:06. C14:07. C16:08. C16:19. C18:010. C18:111. C18:212. C18:312 3 456789101112Fatty Acid Methyl Esters502Figure 87. C18:3, C20:0, and C20:1 Methyl EstersPacking: GP 3% SP-2310/2% SP-2300 on 100/120 Chromosorb W AWCat. No.: 11833 (packing)Column: 6' x 1/8" stainless steelOven: 190°C (2 min) to 220°C at 2°C/minCarrier: nitrogen, 20mL/minDet.: FIDInj.: 5µg mixed esters in 0.5µL methylene chlorideFigure 88. Polyunsaturated FAMEsPacking: 10% SP-2330 on 100/120 Chromosorb W AWCat. No.: 11851 (packing)Column: 6' x 1/8" stainless steelCat. No.: 13775 (general configuration stock column; other stock columns available)Oven: 210°CInj.: 15µg mixed esters in 1.5µL isooctaneFor more information, request Bulletin 856.795-0198For more information, request Bulletin 856.795-01992 4 60 8 10 12 14 16 18 20MIn1354678910111. C14:02. C16:03. C18:04. C18:15. C18:26. C18:37. C20:08. C20:19. C22:010. C22:111. C24:01. C14:02. C16:03. C18:04. C18:15. C20:16. C22:17. C20:58. C24:19. C22:510. C22:60 16MIn1814122 4 6 8 10123 4567810951Figure 89. Reference Standard: RM-3 Peanut Oil TypePacking: 10% SP-2340 on 100/120 Chromosorb W AWCat. No.: 11852 (packing)Column: 2 meter x 2mm ID glassOven: 195°CCarrier: nitrogen, 20mL/minInj.: 0.5µL, 10mg/cc isooctane1. C14:02. C16:03. C18:04. C18:15. C18:26. C18:37. C20:08. C22:09. C22:110. C24:0795-0201Figure 90. Grapefruit OilPacking: 5% SP-2100/0.1% SP-401 on 100/120 SUPELCOPORTCat. No.: 11839 (packing)Column: 6' x 2mm ID glassOven: 75°C (2 min) to 175°C at 4°C/minCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.2µLFAMEs/FragrancesFor more information, request Bulletin 856.795-02001. -Pinene2. Sabinene3. Myrcene4. Limonene5. Unknown6. Nonanal7. Linalool8. Citronellal9. Decanal10. Unknown11. -Copaene12. -Copaene13. -Elemene14. Caryophyllene15. Humulene16. Valencene17. D-Cadinene18. Nootkatone0 2 4 6 8 10 12 14 16Min2143567 89101 32 46,758910 111213141815 16170 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19Min52Figure 91. Lemon OilPacking: 5% SP-2100/0.1% SP-401 on 100/120 SUPELCOPORTCat. No.: 11839 (packing)Column: 6' x 2mm ID glassOven: 75°C (2 min) to 175°C at 4°C/minCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.2µL795-0202Figure 92. Orange OilPacking: 5% SP-2100/0.1% SP-401 on 100/120 SUPELCOPORTCat. No.: 11839 (packing)Column: 6' x 2mm ID glassOven: 75°C (2 min) to 175°C at 4°C/minCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.2µLFor more information, request Bulletin 856.795-02030 2 4 106 8 12 14 16 2018 22 24 26 3028 32 34 36 38Min1 2 345789101112 17136161514 19180Min21. -Thujene2. -Pinene3. Camphene4. Sabinene5. -Pinene6. Myrcene7. Unknown8. p-Cymene9. Limonene10. -Terpinene11. Terpinolene12. Nonanal13. Linalool14. Unknown15. Terpinene-4-ol16. -Terpineol17. Neral18. Geranial19. Neryl acetate20. Geranyl acetate21. Caryophyllene22. Bergamotene23. Unknown24. Unknown25. -Bisabolene302826242220181614121084 61234,5 6789 10111415161718192021222512,1323241. -Pinene2. Sabinene3. Myrcene/Octanal4. Phellandrene5. Limonene6. Unknown7. Nonanal8. Linalool9. Citronellal10. Decanal11. Neral12. Geranial13. Dodecanal14. Caryophyllene15. -Copaene16. Farnesene17. Valencene18. -Sinensal19. -Sinensal53Figure 93. Freon® and Chlorinated HydrocarbonsPacking: 80/100 Carbopack B/1% SP-1000Cat. No.: 11815 (packing)Column: 6' x 1/8" stainless steelCat . No.: 12485 (general configuration stock column;other stock columns available)Oven: 70°CCarrier: nitrogen, 20mL/minDet.: FIDFigure 94. Freon GasesPacking: 60/80 Carbopack C/0.2% Carbowax 1500Cat. No.: 11826 (packing)Column: 6' x 1/8" stainless steelCat . No.: 13860-U (general configuration stock column;other stock columns available)Oven: 65°C to 150°C at 8°C/minCarrier: nitrogen, 20mL/minDet.: FIDFor more information, request Bulletin 786.795-0205For more information, request Bulletin 786.795-0204Fragrances/Freons1. Methyl chloride2. Methyl bromide3. Vinyl chloride & Freon 124. Chloroethane0 1 2 3 4Min43211. Freon 232. Freon 133. Freon 224. Freon 1155. Freon 126. Freon 217. Freon 1148. Freon 119. Freon 1130 2 4 6 8 10 12Min98765423154Figure 95. Freon GasesPacking: 60/80 Carbopack B/5% Fluorcol®Column: 10' x 1/8" SP alloyCat. No.: 12425(general configuration stock column only)Oven: 50°CCarrier: nitrogen, 30mL/minDet.: FIDInj.: 10µL synthetic mixture (appr. 20% each compound)For more information, request Bulletin 786.Figure provided by J.L. Glajch and W.G. Schindel, I.I. du Pont de Nemours and Co., Inc.,Experimental Station, Wilmington, DE, USA.713-0705Figure 96. FormaldehydePacking: 100/120 HayeSep TCat. No.: 10311 (packing, 75cc/bottle)Column: 5' x 1/8" stainless steelOven: 132°CCarrier: helium, 30cc/minDet.: thermal conductivity (175mA)Inj.: 0.2µL (165°C)713-06901. Freon 222. Freon 123. Freon 214. Freon 1145. Freon 114a (impurity)6. Freon 110 2 4 6 8 10 12 14 16 18 20Min324651. Air2. Formaldehyde3. Water4. Methanol0 1 2 3Min1243155Figure 97. Formalin SolutionPacking: 45/60 Carboxen-1000Column: 2' x 1/8" stainless steelCat. No.: 12370-U (general configuration stockcolumn; other stock columns available)Oven: 150°CCarrier: helium, 30mL/minDet.: TCDInj.: 0.2µL formalin solution795-0206Figure 98. Ethylene and Scott® Mix 216Packing: 100/120 HayeSep DBCat. No.: customColumn: 30' x 1/8" stainless steelOven: 120°CCarrier: helium, 30mL/minDet.: P.E. 900 T.C., 300maInj.: 250mL Valco valve, 120°CFor more information, request Application Note 10.795-0207Freons/Formaldehyde/C1-C22311. Water2. Formaldehyde3. Methanol0 2 4Min1. Nitrogen2. Carbon monoxide3. Methane4. Carbon dioxide5. Acetylene (0.24ppm)6. Ethylene7. Water8. Ethane0 2 3 4 5 6 7 98 10 11 12 13Min18765314256Figure 99. Carbon Dioxide and C2 HydrocarbonsPacking: HayeSep TCat. No.: 10311 (packing)Column: 5' x 1/8" stainless steelOven: 32°CCarrier: helium, 30mL/minDet.: 180°CInj.: 50mLFor more information, request Application Note 10.795-0208Figure 100. Mapp Gases, C1-C3 UnsaturatedHydrocarbons and CO2Packing: HayeSep RCat. No.: 10304 (packing)Column: 10' x 1/8" stainless steelOven: 80°CCarrier: helium, 30mL/minInj.: 15mLFor more information, request Application Note 10.795-02091. Air2. Methane3. Carbon dioxide4. Ethylene5. Ethane6. Acetylene0 2 64Min5643211. Air2. Methane3. Carbon dioxide4. Ethane5. Propylene6. Propane7. Propadiene8. Methyl acetylene0 2 4 6 7531Min1523467857Figure 102. C1-C2 HydrocarbonsPacking: 100/120 HayeSep DCat. No.: 10293 (packing)Column: 10' x 1/8" stainless steelOven: 80°CCarrier: helium, 35mL/minDet.: FID (3700 Varian)Figure 101. Scott Mix 216, C1-C2, CO, and CO2Packing: HayeSep DCat. No.: 10293 (packing)Column: 20' x 1/8" NiOven: 40°C (2 min) to 110°C at 24°C/minCarrier: helium, 30mL/minDet.: P.E. 900 T.C., 225ma, 140°CInj.: 100mL, Valco valve, ambientFor more information, request Application Note 10.795-0210C1-C2 Hydrocarbons/GasesFor more information, request Application Note 10.795-02111. Nitrogen (balance)2. Carbon monoxide, 1%3. Methane, 1%4. Carbon dioxide, 1%5. Acetylene, 1%6. Ethylene, 1%7. Ethane, 1%0 2 4 6 108Min21345674 02Min42311. Methane, 1%2. Acetylene, 1%3. Ethylene, 1%4. Ethane, 1%58Figure 103. Trace Acetylene in EthylenePacking: 45/60 Carboxen-1000Column: 5' x 1/8" stainless steelCat. No.: 12380 (general configuration stockcolumn; other stock columns available)Oven: 160°CCarrier: helium, 30mL/minDet.: FIDInj.: 0.5mL ethylene withtrace impuritiesFigure 104. Trace Acetylene in EthylenePacking: 60/80 Carbosieve S-IICat. No.: customColumn: 5' x 1/8" stainless steelOven: 125°CCarrier: nitrogen, 30mL/minDet.: FIDInj.: 1mL, 100ppm acetylene in ethyleneFor more information,request Application Note 10.795-0212For more information,request Application Note 10.795-0213Min0 181614121086421231. Methane2. Acetylene3. EthyleneMin1080 41231. Methane2. Acetylene (14ppm)3. Ethylene59C1-C3 HydrocarbonsMin0 2 4 67654321Figure 106. C1-C3 HydrocarbonsPacking: 60/80 Carbosieve GCat. No.: 10198 (packing)Column: 5' x 1/8" stainless steelOven: 145°C to 195°C at 6°C/minCarrier: nitrogen, 50mL/minDet.: FIDInj.: 1mL, appr. 15ppm eachcomponent in nitrogenFor more information,request Bulletin 743 and Application Note 10.795-0214Figure 105. C1-C3 HydrocarbonsPacking: 45/60 Carboxen-1000Column: 2' x 1/8" stainless steelCat. No.: 12370-U (general configuration stockcolumn; other stock columns available)Oven: 165°C (1 min) to 220°C at 16°C/minCarrier: helium, 60mL/minDet.: FIDInj.: 0.6mL Scott Gas Mix (Cat. No. 2-3470),15ppm each component, appr. 10ngeach component on column1. Methane2. Acetylene3. Ethylene4. Ethane5. Propyne6. Propylene7. PropaneFor more information,request Bulletin 743 and Application Note 10.795-0215121086420Min1. Methane2. Acetylene3. Ethylene4. Ethane5. Propyne6. Propylene7. Propane765432160For more information, request Bulletin 743and Application Note 10.795-0216For more information, request Bulletin 743and Application Note 142.713-0757Figure 108. C3-C5 Saturated and Unsaturated HydrocarbonsPacking: 80/100 Carbopack C/0.19% picric acidCat. No.: 11824 (packing)Column: 2m x 1/8" stainless steelCat. No.: 13867 (general configuration stock column only)Oven: 35°C to 70°C at 6°C/minCarrier: nitrogen, 30mL/minDet.: FIDFigure 107. Methanol in Propylene/PropanePacking: 100/120 HayeSep TCat. No.: 10311 (packing)Column: 5' x 1/8" stainless steelOven: 120°CCarrier: helium, 30mL/minDet.: P.E. 900 T.C., 225ma, 150°CInj.: 132°C1. Air, Methane2. Carbon dioxide3. Ethane4. Propylene, Propane5. Butane6. Butane7. Methanol543210Min7652 3 411. Propane2. Propylene3. Isobutane4. 1-Butene5. n-Butane6. Isobutylene7. cis-Butene-28. trans-Butene-29. 1,3-Butadiene10. Isopentane11. n-PentaneMin3456789101112106 14 1684201,261C1-C5 HydrocarbonsFigure 109. C3-C5 Saturated and Unsaturated HydrocarbonsPacking: 10% EDO-1 on 100/120 Chromosorb P AWCat. No.: 11967 (packing)Column: 20' x 1/8" stainless steelOven: 0°CCarrier: nitrogen, 40mL/minDet.: FIDInj.: ASTM Hydrocarbon MixSection L, Blend 6For more information, requestBulletin 743 and Application Note 142.795-021721345678911101. Propane2. Propylene3. Isobutane4. n-Butane5. 1-Butene6. Isobutylene7. trans-2-Butene8. Isopentane9. cis-2-Butene10. n-Pentane11. 1,3-Butadiene14121086420MinFigure 110. C1-C5 Saturated and Unsaturated HydrocarbonsPacking: 15% EDO-1 on 60/80 Chromosorb P AWCat. No.: customColumn: 20' x 3/16" stainless steelCarrier: nitrogen, 40mL/minFor more information, request Bulletin 743 and Application Note 10 and 142.795-021811. Isopentane12. cis-2-Butene13. n-Pentane14. 3-Methyl-1-butene15. 1,3-Butadiene16. 1-Pentene17. 2-Methyl-1-butene18. trans-2-Pentene19. cis-2-Pentene20. 2-Methyl-2-butene1. Methane2. Ethane3. Ethylene4. Propane5. Propylene6. Isobutane7. n-Butane8. 1-Butene9. Isobutylene10. trans-2-Butene01324567 8910111213141516192018174 8 12 16 20 24 28 32 36 38Min62Figure 111. Trace C2-C6 HydrocarbonsPacking: 100/120 HayeSep DCat. No.: 10293 (packing)Column: 10' x 1/8" stainless steelOven: 120°C to 200°C at 24°C/minCarrier: helium, 35mL/minDet.: FID (3700 Varian)MinFigure 112. C1-C5 HydrocarbonsPacking: 100/120 HayeSep DCat. No.: 10293 (packing)Column: 10' x 1/8" stainless steelOven: 120°C to 200°C at 20°C/minCarrier: helium, 35mL/minDet.: FID (3700 Varian)For more information, request Bulletin 743 and Application Note 10 and 142.795-0220For more information, request Bulletin 743 and Application Note 10 and 142.795-021901234567891011121314156541. Acetylene 16ppm2. Ethylene 15ppm3. Propylene 14.3ppm4. 1-Butene 15ppm5. 1-Pentene 14.75ppm6. 1-Hexane 16ppm325 4 3 2 1 0Min1. Methane 0.1894%2. Ethane 0.0965%3. Propane 0.0989%4. Isobutane 0.1019%5. Butane 0.1019%6. n-Pentane 0.2002%6543 2 17 6891011121632C1-C6 HydrocarbonsFor more information, request Bulletin 743, and Application Note 142.713-0758Figure 113. C1-C6 Saturated and Unsaturated HydrocarbonsPacking: 23% SP-1700 on 80/100 Chromosorb P AWCat. No.: 11865 (packing)Column: 30' x 1/8" stainless steelCat. No.: 12809-U (general configuration stock column only)Oven: 70°CCarrier: helium, 25mL/minDet.: FID, 150°CInj.: 0.6µL ASTM Section L, Blend 6,plus C5 hydrocarbons, 100°CFigure 114. Propane and Propyleneat High ConcentrationsPacking: 23% SP-1700 on 80/100 Chromosorb P AWCat. No.: 11865 (packing)Column: 30' x 1/8" stainless steelCat. No.: 12809-U (general configuration stock column only)Oven: 70°CCarrier: helium, 25mL/minDet.: FID, 150°CInj.: 0.5mL liquid propane gas, 100°CFor more information, request Bulletin 743 and Application Note 142.795-02211. Ethane2. Propane3. Propylene4. Isobutane5. n-Butane6. 1-Butene, Isobutylene7. trans-2-Butene8. cis-2-Butene9. Isopentane10. 1,3-Butadiene11. Pentane12. 1-Pentene13. 2-Methyl-1-butene, trans-2-Pentene14. cis-2-Pentene15. 2-Methyl-2-butene16. 2-Methyl-1-pentane17. 3-Methyl-1-pentane18. n-HexaneU Unknown19. 3-Methylhexane0 2 10 12 144 16 18 20 22 24 26 28 30Min6 821 34 5 6 7891012131415 171618U19110 12108642Min68574311. Pressure disturbance2. Ethane 0.42%3. Propane 96.75%4. Propylene 1.72%5. Isobutane 0.93%6. n-Butane 0.17%7. 1-Butene, Isobutylene trace8. Isopentane trace64Figure 115. Industrial Butane IIIPacking: 23% SP-1700 on 80/100 Chromosorb P AWCat. No.: 11865 (packing)Column: 30' x 1/8" stainless steelCat. No.: 12809-U (general configurationstock column only)Oven: 70°CCarrier: helium, 25mL/minDet.: FID, 110°CInj.: 0.75mL industrial butane IIIFor more information, request Bulletin 743 and Application Note 142.713-0739Figure 116. Permanent Gases in AirPacking: 60/80 Molecular Sieve 5ACat. No.: 20302 (packing)Column: 3' x 1/8" stainless steelOven: 60°CCarrier: helium, 20mL/minDet.: thermal conductivity, 150maInj.: 0.25cc, 1% concentrations ofH2, O2, CH4, and CO in N2For more information, request Bulletin 743 and Application Note 142.795-02221. Pressure disturbance2. Isobutane 2.02%3. n-Butane 97.92%4. Isopentane 0.05%5. n-Pentane 0.01%0 2 4 6 8 10 1412 16Min2 35411. H22. O23. N24. CH45. CO0 1 2 3 4 5 6Min5413265Permanent Gas/HydrocarbonsFigure 117. Permanent Gases in AirPacking: 60/80 Chromosorb 102Cat. No.: 20200-U (packing)Column: 6' x 1/8" stainless steelOven: 60°CCarrier: helium, 20mL/minDet.: thermal conductivity, 150maInj.: 0.2cc, 1% concentrations of CH4, and CO2 from N2Figure 118. Scott Mix 237Packing: 100/120 HayeSep DCat. No.: 10293 (packing)Column: 20' x 1/8" NiOven: 25°CCarrier: helium, 30cc/minDet.: P.E. 900 T.C, 225ma, 140°CInj.: 50mL vapor, Valco valve,ambient01. Nitrogen balance2. Oxygen 7%3. Carbon monoxide 7%4. Methane 4.5%5. Carbon dioxide 15%1. H2, O2, N2, & CO2. CH43. CO24. H2O14321 2 3 4MinFor more information, request Application Note 10.795-022314532Min1 53 7 11 13 17159795-022466Figure 119. Nitrogen in ArgonPacking: 100/120 HayeSep DCat. No.: 10293 (packing)Column: 30' x 1/8" stainless steelOven: 25°CCarrier: helium, 30cc/minDet.: P.E. 900 T.C, 300ma, 140°CInj.: 250mL Valco valve, 25°C795-0226Figure 120. Permanent GasesPacking: 80/100 HayeSep ACat. No.: 10283 (packing)Column: 36' x 1/8"Oven: 25°CCarrier: helium, 23cc/minDet.: P.E. 900 T.C, 225maInj.: 25mL air plus hydrogen and carbon monoxide0 1 2 3 4 5 6 71. Nitrogen 373ppm2. Argon balance795-0225120 1 2 3 4 5 6 7 8 9Min1. Hydrogen 5%2. Nitrogen 48.5%3. Oxygen 13%4. Argon 0.5%5. Carbon monoxide 33%2531467Permanent Gas/HydrocarbonsFigure 121. Permanent Gases, Methane,and C2 HydrocarbonsPacking: 100/120 Carbosieve S-IIColumn: 10' x 1/8" stainless steelCat. No.: 13821 (general configuration stock column;other stock columns available)Oven: 35°C (7 min) to 225°Cat 32°C/minCarrier: helium, 30mL/minDet.: thermal conductivity,150maInj.: 0.6mLFor more information, request Application Note 10.795-02271. Hydrogen 2.5%2. Oxygen 2.5%3. Nitrogen 91.4%4. Carbon monoxide 0.6%5. Methane 0.6%6. Carbon dioxide 0.6%7. Acetylene 0.6%8. Ethylene 0.6%9. Ethane 0.6%Figure 122. Permanent Gases and C1-C2 HydrocarbonsPacking: 60/80 Carboxen-1000Column: 15' x 1/8" stainless steelCat. No.: 12390-U (general configuration stock column;other stock columns available)Oven: 35°C (5 min) to 225°C at 20°C/minCarrier: helium, 30mL/minDet.: TCDInj.: 0.6mL Scott Gas Mix (Cat. No. 23437) with oxygen added, 1% eachcomponent in nitrogen, on columnFor more information,request Application Note 10.795-022880 4 1612 20 24Min13456 7 81. Oxygen2. Nitrogen3. Carbon monoxide4. Methane5. Carbon dioxide6. Acetylene7. Ethylene8. Ethane20 2 4 6 8 10 12 14 1816 2220 24Min125479863680Figure 123. PesticidesPacking: GP 1.5% SP-2250/1.95% SP-2401 on 100/120 SUPELCOPORTCat. No.: 11947 (packing)Column: 6' x 4mm ID glass(stock column available)Oven: 200°CCarrier: argon:methane (95:5), 70mL/minDet.: EC NI63Inj.: 5µL CP MixFor more information, request Bulletins 758 and 775.795-02290 2 4 6 108 12 14 16 18Min123547689101112131. -BHC2. -BHC (Lindane)3. -BHC4. Heptachlor5. Aldrin6. Heptachlor epoxide7. 4,4'-DDE8. Dieldrin9. 2,4-DDD (TDE)10. Endrin11. 2,4'-DDT12. 4,4'-DDD (TDE)13. 4,4'-DDTFor more information, request Bulletins 758 and 775.795-02301. -BHC2. -BHC (Lindane)3. -BHC4. Heptachlor5. Aldrin6. Heptachlor epoxide7. 4,4'-DDE8. 2,4-DDD (TDE)9. Dieldrin10. 2,4'-DDT11. Endrin12. 4,4'-DDD (TDE)13. 4,4'-DDT2 4 6 8 10 12 14 16 18 20Min12 354678910 111213Figure 124. PesticidesPacking: GP 4% SE-30/6% SP-2401 on 100/120 SUPELCOPORTCat. No.: 11948 (packing)Column: 6' x 4mm ID glassOven: 200°CCarrier: argon:methane (95:5), 70mL/minDet.: EC NI63Inj.: 5µL CP Mix69Pesticides/PhenolsFigure 125. PhenolsPacking: 0.1% SP-1000 on 80/100 Carbopack CCat. No.: 11820 (packing)Column: 6' x 2mm ID glass (stock column available)Oven: 225°CCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.5µL approx. 0.1% each in MEKFigure 126. Mixed PhenolsPacking: 3% SP-1000 on 100/120 SUPELCOPORTCat. No.: 11738 (packing)Column: 6' x 2mm ID glassOven: 130°CCarrier: nitrogen, 20mL/minDet.: FID, 175°CInj.: 0.05µL1. Phenol2. o-Cresol3. m-Cresolp-Cresol4. 2,6-Dimethylphenol5. 2,4-Dimethylphenol2,5-Dimethylphenol6. 3,5-Dimethylphenol7. 2,3-Dimethylphenolm-Ethylphenolp-Ethylphenol8. 3,4-Dimethylphenol9. 2,4,6-Trimethylphenol10. 2,3,6-Trimethylphenol11. 2,3,5-Trimethylphenol12. 2,4,5-Trimethylphenol0 2 64 108 12 14 16 18 20 11Min123456 7 891. Phenol2. o-Cresol3. m-Cresol4. p-Cresol5. o-Ethylphenol6. m-Ethylphenol7. p-Ethylphenol8. 2,6-Dimethylphenol9. 2,4-Dimethylphenol, 2,5-Dimethylphenol10. 2,3-Dimethylphenol, 3,5-Dimethylphenol11. 3,4-Dimethylphenol1110For more information, request Bulletin 775.795-0231For more information, request Bulletin 775.795-02320 1 32 4 5 6 7 8 10 119 12Min123456 7891011 1270Figure 127. PhenolsPacking: 60/80 Carbopack F-TAColumn: 2m x 2mm ID glassOven: 220°CCarrier: nitrogen, 15mL/minDet.: FIDInj.: 0.2µL approx. 0.1% each in MEKFigure 128. Butyl PhenolsPacking: 10% SP-1000 on 80/100 SUPELCOPORTCat. No.: 11872 (packing)Column: 6' x 1/8" stainless steelOven: 180°CCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.5µL with 0.1% of eachcomponent in chloroform795-0233For more information,request Bulletin 775.794-013920 4 6 8 10Min11987652341S10S Solvent1. Phenol2. o-Cresol3. m-Cresol4. p-Cresol5. o-Ethylphenol6. p-Ethylphenol7. p-Ethylphenol8. 2,6-Dimethylphenol9. 2,4-Dimethylphenol2,5-Dimethylphenol10. 2,3-Dimethylphenol3,5-Dimethylphenol11. 3,4-Dimethylphenol1. 2,6-Di-tert-butylphenol2. 2,6-Di-tert-butyl-p-cresol0 1 2 3 4 5 6MIn217120Phenols/PheromonesFigure 129. Phenols (Anisoles)Packing: 5% SP-1200/1.75% Bentone 34Cat. No.: 12134 (packing)Column: 6' x 1/8" stainless steelCat. No.: 12721 (general configuration stock column;other stock columns available)Oven: 150°CCarrier: nitrogen, 20mL/min1. Anisole2. o-Methylanisole3. p-Methylanisole4. 2,6-Dimethylanisole5. m-Methylanisole6. 2,4-Dimethylanisole7. 2,3-Dimethylanisole8. 2,5-Dimethylanisole9. 3,4-Dimethylanisole10. 3,5-Dimethylanisole795-0238Figure 130. PheromonesPacking: 15% SP-2340/ on 100/120 Chromosorb P AW-DMCSCat. No.: customColumn: 20' x 1/8" stainless steelOven: 225°CCarrier: nitrogen, 10mL/min at 50psiInj.: E and Z-9-tetradecenyl acetate795-02340 1 2 3 54 76Min123,456879 10ZE32Min2824Z72Figure 131. SIMDIS Calibration Blend (ASTM D3710)Column: Petrocol™ A, 20" x 1/8" stainless steelCat. No.: 12445 (general configuration stock column only)Oven: -20°C to 200°C at 20°C/minCarrier: helium, 25mL/minDet.: FID, 300°CInj.: 0.1µL of D3710 QualitativeCalibration Mix(Cat. No. 48884), 300°CFigure 132. Gasoline by ASTM D3710Column: Petrocol A, 20" x 1/8"stainless steelCat. No.: 12445 (general configurationstock column only)Oven: -20°C to 250°C at 15°C/minCarrier: helium, 25mL/minDet.: FID, 300°CInj.: 0.1µL gasoline, 300°C1. C5s2. C6s3. C7s4. Toluene5. Xylenes1. n-Propane2. Methylpropane3. n-Butane4. 2-Methylbutane5. n-Pentane6. 2-Methylpentane7. n-Hexane8. 2,4-Dimethylpentane9. n-Heptane10. Toluene11. n-Octane12. p-Xylene13. n-Propylbenzene14. n-Decane15. n-Butylbenzene16. n-Dodecane17. n-Tridecane18. n-Tetradecane19. n-Pentadecane0 1 2 3 4 5 6 7 8 9 10MinFor more information, request Bulletin 864.713-0772123 4578691011121314 1617 191815108642014523MinFor more information,request Bulletin 864.713-077173Figure 133. SIMDIS Calibration Blend (ASTM D2887)Column: Petrocol B, 20" x 1/8" stainless steelCat. No.: 12449 (general configuration stock column only)Oven: -25°C to 350°C at 15°C/minCarrier: helium, 30mL/minDet.: FIDInj.: 0.1µL Quantitative Calibration Mix (Cat. No. 48882)Figure 134. Reference Gas Oil Sample (ASTM D2887)Column: Petrocol B, 20" x 1/8" stainless steelCat. No.: 12449 (general configuration stock column only)Oven: -25°C to 350°C at 15°C/minCarrier: helium, 30mL/minDet.: FIDInj.: 0.1µL Reference Gas Oil Sample (Cat. No. 48873).Simulated Distillation (SIMDIS)1. nC62. nC73. nC84. nC95. nC106. nC117. nC128. nC149. nC1610. nC1811. nC2012. nC2413. nC2814. nC3215. nC3616. nC4017. nC44For more information, request Bulletin 864.713-07690 4 8 12 16 20MinFor more information, request Bulletin 864.712-04270 2 4 6 8 10 1412 16 18 20 22 24Min13245 6 7891011 1213 14 15 16 1774Figure 135. PS-18-44D Wax Standard Mix (ASTM D2887)Column: Petrocol B, 20" x 1/8" stainless steelCat. No.: 12449 (general configuration stock column only)Oven: 80°C to 350°C at 10°C/minCarrier: nitrogen, 30mL/minDet.: FID, 380°CInj.: 1µL PS-18-44D Mix (Cat. No. 48928), 350°CFigure 136. PS-18-60D Wax Standard Mix (ASTM D2887)Column: Petrocol B, 20" x 1/8" stainless steelCat. No.: 12449 (general configuration stock column only)Oven: 50°C (1 min) to 350°C at 35°C/minCarrier: nitrogen, 60mL/minDet.: FID, 380°CInj.: 0.5µL PS-12-60D Mix (Cat. No. 48932), 350°C1. n-Dodecane2. n-Tetradecane3. n-Hexadecane4. n-Octadecane5. n-Eicosane6. n-Docosane7. n-Tetracosane8. n-Hexacosane9. n-Octacosane10. n-Triacontane11. n-Dotriacontane12. n-Hexatriacontane13. n-Tetracontane14. n-Tetratetracontane15. n-Pentacontane16. n-HexacontaneFor more information, request Bulletin 864.794-04081. n-Octadecane2. n-Eicosane3. n-Docosane4. n-Tetracosane5. n-Hexacosane6. n-Octacosane7. n-Triacontane8. n-Dotriacontane9. n-Hexatriacontane10. n-Tetracontane11. n-Tetratetracontane2 4 6 8 10 12 14 16 18 20 2422 26Min12345 76891011794-01650 2 4 6 8 10 12 14Min1243567891011121314151675Figure 137. Crude Oil Qualitative Calibration StandardColumn: Petrocol C, 20" x 1/8" stainless steelCat. No.: 12455 (general configurationstock column only)Oven: -25°C to 350°C at 15°C/minCarrier: helium, 30mL/minDet.: FID, 360°CInj.: 1µL Crude Oil QualitativeCalibration Standard, 360°CFigure 138. Crude Oil — North Sea, OsebergColumn: Petrocol C, 20" x 1/8"stainless steelCat. No.: 12445 (general configuration stock column only)Oven: -25°C to 350°C at 15°C/minCarrier: helium, 30mL/minDet.: FID, 360°CInj.: 1µL oil:CS mix (1:1), 360°CSimulated Distillation (SIMDIS)For more information, request Bulletin 864.711-00720 4 8 12 16 20 24 28Min4563178 91718192012131415162212223For more information, request Bulletin 864.711-0076,0077Min001. Porpane (nC3), 1.0%2. n-Butane (nC4)3. n-Pentane (nC5), 3.4%4. n-Hexane (nC6), 5.2%5. n-Heptane (nC7)6. n-Octane (nC8), 6.2%7. n-Nonane (nC9), 4.7%8. n-Decane (nC10)9. n-Undecane (nC11)10. n-Dodecane (nC12)11. n-Tridecane (nC13)12. n-Tetradecane (nC14)13. n-Pentadecane (nC15)14. n-Hexadecane (nC16)15. n-Heptadecane (nC17)16. n-Octadecane (nC18)17. n-Eicosane (nC20)18. n-Tetracosane (nC24)19. n-Octacosane (nC28)20. n-Dotriacontane (nC32)21. n-Hexatriacontane (nC36)22. n-Tetracontane (nC40)23. n-Tetratetracontane (nC44)4.4% each by weight, except as noted.40 8 12 16 20 24Without Internal StandardWith Internal Standard1. Tetradecane (nC14)2. Pentadecane (nC15)3. Hexadecane (nC16)4. Heptadecane (nC17)1 324101176Figure 139. SIMDIS, Regular GasolinePacking: 10% SP-2100 on 100/120 SUPELCOPORTCat. No.: 12140 (packing)Column: 10' x 1/8" stainless steelCat. No. 13766 (general configuration stock column;other stock columns available)Oven: 75°C to 200°C at 8°C/minCarrier: 20mL/minDet.: FIDInj.: 0.1µLFigure 140. NaphthaPacking: 10% SP-2100 on 100/120 SUPELCOPORTCat. No.: 12140Column: 10' x 1/8" stainless steelCat. No.: 13766 (general configuration stock column;other stock columns available)Oven: 75°C to 200°C at 8°C/minCarrier: 20mL/minDet.: FIDInj.: 0.1µLFor more information, request Bulletin 864.711-0087711-00881. C62. C83. C104. C125. C146. C16For more information,request Bulletin 864.MIn0 2 4 6 8 10 121 2 431. C62. C83. C104. C121 2 3 4 5 620 4 6 8 10 12 14 16 18MInFractionFraction77Figure 142. Microcrystalline WaxPacking: 1% Dexsil® 300 on 100/120 SUPELCOPORTCat. No.: 11972 (packing, 20g/bottle)Column: 18' x 1/8" stainless steelOven: 175°C to 350°C at 8°C/minCarrier: nitrogen, 20mL/minDet.: 350°CInj.: 1µL chloroform containing 30µg wax (325°C)Figure 141. Kerosene (Jet A)Packing: 10% SP-2100 on 100/120 SUPELCOPORTCat. No.: 12140 (packing)Cat. No.: 13766 (general configuration stock column;other stock columns available)Column: 10' x 1/8" stainless steel(stock column available)Oven: 75°C to 200°C at 8°C/minCarrier: 20mL/minDet.: FIDInj.: 0.1µLPermanent Gas/HydrocarbonsFor more information, request Bulletin 864.713-07500 20MIn242218161412108642For more information, request Bulletin 864.711-0089MIn0 2 4 6 8 10 12 14 16 18 20 22 241 2 3 4 5 61. C62. C83. C104. C125. C146. C16Fraction78Figure 143. WaxPacking: 3% Dexsil® 300 on 100/120 SUPELCOPORTCat. No.: 11973 (packing)Column: 6' x 1/8" stainless steelOven: 100°C to 360°C at 4°C/minCarrier: nitrogen, 20mL/minFigure 144. Solvents — Alcohol Retarder ColumnPacking: 15% THEED on 100/120 Chromosorb W AWCat. No.: 11823 (packing)Column: 10' x 1/8" stainless steelOven: 80°CCarrier: nitroDet.: FIDInj.: 0.5µLgen, 20mL/minFor more information, request Bulletins 816 and 824.795-0243For more information, request Bulletin 864.795-0242MIn1. C102. C123. C144. C165. C186. C207. C228. C249. C2610. C2811. C3012. C3213. C3414. C3615. C3816. C4017. C441 32 45678910111213141516170 4 8 12 16 20 24 28 32 36 40 44 48 52 56 600MIn1 2 3 4 5 6 7 8 91. Heptane2. Octane3. Ethyl acetate4. n-Propyl acetate5. Toluene6. Ethyl benzene7. Xylene8. Isopropyl alcohol9. Ethyl alcohol10. n-Propyl alcohol123 4578 9610Fraction79Figure 145. SolventsPacking: 60/80 Carbowax C/0.2% Carbowax 1500Cat. No.: 11826 (packing)Column: 6' x 1/8" stainless steelCat. No.: 13860-U (general configuration stock column, other stock columns available)Oven: 100°CCarrier: nitrogen, 20mL/minDet.: FIDInj.: 1.0µLFigure 146. Solvents (Methanol through Pentanol)Packing: GP 80/100 Carbopack C/0.2% Carbowax 1500Cat. No.: 11826 (packing)Column: 6' x 2mm stainless steelCat. No.: 13860-U (general configuration stock column;other stock columns available)Oven: 125°CCarrier: nitrogen, 20mL/min, 31psiDet.: FIDInj.: 0.02µLFor more information, request Bulletins 816 and 824.795-02391. Methanol2. Ethanol3. 2-Propanol4. 1-Propanol5. 2-Methyl-2-propanol (1-butyl)6. 2-Butanol (sec-butyl)7. 2-Methyl-1-propanol (iso)8. 1-Butanol9. 2-Methyl-2-butanol (t-amyl)10. 2,2-Dimethyl-1-propanol11. 3-Methyl-2-butanol12. 3-Pentanol13. 2-Pentanol14. 2-Methyl-1-butanol (active)15. 3-Methyl-1-butanol16. 1-PentanolFor more information,request Bulletins 816 and 824.795-02440Min1 2 3 4 5 6 7 8124567 8910 12111413161530 1 2 3 5 6 7 89785643211. Methanol2. Ethanol3. Acetone4. Isopropyl alcohol5. Ethyl ether6. Ethyl acetate7. Methyl ethyl ketone8. Isobutyl alcohol9. Benzene4Solvents80Figure 147. Solvents (Ethyl Ether Through Styrene)Packing: 10% SP-1000 on 80/100 SUPELCOPORTCat. No.: 11872 (packing)Column: 20' x 1/8" stainless steelCat. No.: 12719 (general configuration stock column;other stock columns available)Oven: 100°C (6 min) to 140°C at 20°C/minCarrier: nitrogen, 30mL/minDet.: FIDInj.: 5µL CS2 approx.100ppm each solventFigure 148. Solvents (Methanol Through Xylenes)Packing: 20% SP-2100/0.1% Carbowax 1500 on 100/120 SUPELCOPORTCat. No.: 11821 (packing)Column: 10' x 1/8" stainless steelCat. No.: 12718 (general configuration stock column;other stock columns available)Oven: 100°CCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.5µL, approx. equal proportionsof neat compounds795-02401. Methanol2. Ethanol3. Acetone4. Methyl ethyl ketone5. Ethyl acetate6. Isobutyl alcohol7. Methyl Cellosolve8. Isopropyl acetate9. 2-Nitropropane10. MIBK11. Isobutyl acetate12. Toluene13. n-Butyl acetate14. Diacetone alcohol15. Ethylbenzene16. m- & p-Xylene17. Cellosolve acetate18. Butyl Cellosolve & o-Xylene0 1 2 3 54 6Min7 8 109 11 12 13123456789 10 11121314 16171815For more information, request Bulletins 816 and 824.795-024130 1 8765Min8910111213141817 192034126571516For more information, request Bulletins 816 and 824.1. Ethyl ether2. Isooctane3. Carbon disulfide4. Acetone5. Carbon tetrachloride,Methylchloroform6. Methyl ethyl ketone7. Methylene chloride8. Benzene9. Trichloroethylene10. Chloroform11. Tetrachloroethylene12. Toluene13. Ethylene dichloride14. Dioxane15. Ethylbenzene16. p-Xylene17. m-Xylene18. o-Xylene19. 1,1,2-Trichloroethane20. Styrene8 1Figure 149. Solvents — Ketone-Retarding ColumnPacking: 20% SP-2401/0.1% Carbowax 1500 on 100/120 SUPELCOPORTCat. No.: 11822 (packing)Column: 10' x 1/8" stainless steelOven: 100°CCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.1µL, approx. equal proportionsof neat componentsFigure 150. Industrial SolventsPacking: 80/120 Carbopack B/3% SP-1500Cat. No.: 11813-U (packing)Column: 10' x 1/8" stainless steelCat. No.: 12592 (general configuration stock column;other stock columns available)Oven: 70°C to 225°C at 4°C/minSolvents0 4123467,859101112181619202122Impurity1723,24,252627 282930323116128 16 16 16 16 16 16 16Min713-0787For more information,request Bulletins 816 and 824.795-02450 1 2 3 4 5 6 7 8Min213 546781. Isopropyl alcohol2. Ethyl acetate3. Methyl ethyl ketone4. Toluene5. n-Propyl acetate6. Isobutyl acetate7. MIBK8. Mesityl oxideFor more information,request Bulletin 824.1. Methanol2. Methyl formate3. Ethanol4. Acetone5. Methylene chloride6. Isopropyl alcohol7. Ethyl formate8. Methyl acetate9. n-Propyl alcohol10. Tetrahydrofuran (THF)11. Methyl ethyl ketone(MEK)12. Methyl Cellosolve13. sec-Butyl alcohol14. Ethyl acetate15. Isobutyl alcohol16. 2-Nitropropane17. n-Butyl alcohol18. Ethyl Cellosolve19. n-Propyl acetate20. sec-Butyl acetate21. Cyclohexanone22. Isobutyl acetate23. n-Butyl acetate24. Toluene25. Mesityl oxide26. Cellosolve acetate27. Butyl Cellosolve28. Isoamyl acetate29. Ethylbenzene30. m-Xylene31. p-Xylene32. o-Xylene1513148 2Figure 151. Industrial SolventsPacking: 60/80 Carbopack B/1% SP-1510Cat. No.: 11809 (packing)Column: 10' x 1/8" stainless steelOven: 100°C to 225°C at 8°C/minCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.3µL, approx. equal proportions of neat components1. Methanol2. Methyl formate3. Ethanol4. Methylene chloride5. Acetone6. Isopropyl alcohol7. Ethyl formate8. Methyl acetate9. n-Propyl alcohol10. Tetrahydrofuran (THF)21. sec-Butyl acetate22. Isobutyl acetate23. n-Butyl acetate24. Mesityl oxide25. Toluene26. Cellosolve acetate27. Butyl Cellosolve28. Isoamyl acetate29. Ethylbenzene30. m-Xylene31. o-Xylene32. p-Xylene11. Methyl ethyl ketone (MEK)12. Methyl Cellosolve13. sec-Butyl alcohol14. Isobutyl alcohol15. Ethyl acetate16. 2-Nitropropane17. n-Butyl alcohol18. Ethyl Cellosolve19. n-Propyl acetate20. Cyclohexanone713-0788For more information, request Bulletin 824.0 2 6 8 10 14 22 24 28 30 32 34Min4 12 1816 20 26 36132 45 610111378,9121416151718201921222526,27293031,32Impurity232428Figure 152. Industrial SolventsPacking: 80/100 Carbopack C/0.1% SP-1000Cat. No.: 11820 (packing)Column: 10' x 1/8" stainless steelOven: 70°C to 225°C at 4°C/minCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.3µL, 3% each component byvolume (approx. 12µg each)For more information, request Bulletin 824.713-07891. Methanol2. Methyl formate3. Ethanol4. Acetone5. Methylene chloride6. Isopropyl alcohol7. Ethyl formate8. Methyl acetate9. Tetrahydrofuran (THF)10. n-Propyl alcohol11. Methyl ethyl ketone (MEK)12. Methyl Cellosolve13. sec-Butyl alcohol14. Isobutyl alcohol15. Ethyl acetate16. 2-Nitropropane17. n-Butyl alcohol18. Ethyl Cellosolve19. n-Propyl acetate20. Cyclohexanone21. sec-Butyl acetate22. Isobutyl acetate23. n-Butyl acetate24. Toluene25. Mesityl oxide26. Cellosolve acetate27. Butyl Cellosolve28. Isoamyl acetate29. Ethylbenzene30. m-Xylene31. p-Xylene32. o-Xylene20 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 44 46Min23456 910111171819202122 2324,252726293031,321228Impurity161513147,88 3Solvents/AlcoholsFigure 153. Industrial SolventsPacking: 60/80 Carbopack F-TAColumn: 2m x 2mm ID glassOven: 75°C (2 min) to 225°Cat 4°C/minCarrier: nitrogen, 20mL/minDet.: FIDInj.: 0.05µL solvents mix,3% each solventFigure 154. Alcohols, C1-C5Packing: 80/100 Carbopack F-SLColumn: 2m x 2mm ID TightSpec glassOven: 130°CCarrier: nitrogen, 15mL/minFor more information, request Bulletin 824.713-1069For more information, request Bulletin 824.795-02480Min12. Methyl Cellosolve13. sec-Butyl alcohol14. Ethyl acetate15. Isobutyl alcohol16. 2-Nitropropane17. n-Butyl alcoholI Impurity18. Ethyl Cellosolve19. n-Propyl acetate20. Cyclohexanone21. sec-Butyl acetate22. Isobutyl acetate23. n-Butyl acetate24. Toluene25. Mesityl oxide26. Cellosolve acetate27. Butyl Cellosolve28. Isoamyl acetate29. Ethylbenzene30. m-Xylene31. p-Xylene32. o-Xylene1. Methanol2. Methyl formate3. Ethanol4. Acetone5. Methylene chloride6. Isopropyl alcohol7. Ethyl formate8. Methyl acetate9. n-Propyl alcohol10. Tetrahydrofuran(THF)11. Methyl ethylketone (MEK)2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32134691057,82 12131114,151718 19202122I232428,29 3016 I250 1 2 3 4 5Min123475986101112131415161. Methanol2. Ethanol3. i-Propanol4. n-Propanol5. t-Butanol6. i-Butanol7. 2-Methyl-1-propanol8. n-Butanol9. 2-Methyl-2-butanol10. 2,2-Dimethyl-1-propanol11. 3-Methyl-2-butanol12. 3-Pentanol13. 2-Pentanol14. 2-Methyl-1-butanol15. 3-Methyl-1-butanol16. n-Pentanol31,3226,278 4Figure 155. SugarsPacking: 3% SP-2330 on 100/120 SUPELCOPORTCat. No.: 11802 (packing)Column: 6' x 2mm ID glassOven: 225°CCarrier: nitrogen, 20mL/minDet.: FIDInj.: 5µLFigure 156. SugarsPacking: 3% SP-2340 on 100/120 SUPELCOPORTCat. No.: 11863 (packing)Column: 6' x 2mm ID glassOven: 225°CCarrier: nitrogen, 20mL/minDet.: FIDInj.: 4µL795-02470 2 4 6 8 10 12Min14 16 18 20 22 2412345678 9 111013121. Glyceraldehyde2. Erythrose3. Deoxyribose4. Rhamnose5. Fucose6. Ribose7. Arabinose8. Xylose9. Deoxyglucose10. Mannose11. Galactose12. Glucose13. Inositol795-02491. Glyceraldehyde2. Erythrose3. Deoxyribose4. Rhamnose5. Fucose6. Ribose7. Arabinose8. Xylose9. Deoxyglucose10. Mannose11. Galactose12. Glucose13. Inositol20 4 6 8 10 12 14 16 2018Min13121011978653,4218 5Sugars/Sulfur & MercaptansFigure 157. Sulfur Dioxide in NitrogenPacking: 45/60 Carboxen-1000Column: 2' x 1/8" stainless steelCat. No.: 12370-U (general configuration stock column;other stock columns available)Oven: 190°CCarrier: helium, 30mL/minDet.: TCDInj.: 1µL of 1000ppm SO2 in N2,approx. 1µg SO2 on columnFigure 158. Sulfur Gases and HydrocarbonsPacking: HayeSep QCat. No.: 10301-U (packing)Column: 8' x 1/8"Oven: 90°CCarrier: helium, 30cc/minFor more information, request Bulletin 722.For more information, request Bulletin 722.795-0250211 20Min1. Nitrogen2. Sulfur dioxide1. Air2. Methane3. Carbon dioxide4. Ethylene5. Acetylene6. Ethane7. Hydrogen sulfide8. Carbonyl sulfide9. Propylene10. Propane11. Propadiene2 4 6Min09101187635421795-02518 6Figure 159. Hydrogen Sulfide, Carbon Dioxide, and WaterPacking: 100/120 HayeSep DCat. No.: 10293 (packing)Column: 10' x 1/8" NiOven: 60°CCarrier: helium, 30cc/minDet.: P.E. 900 T.C., 225ma, 140°CInj.: Valco valve, 50 microlitersvapor (ambient), 100°CFigure 160. Hydrogen Sulfide, Nitrogen, Carbon Dioxide,Nitrous Oxide and WaterPacking: 100/120 HayeSep DCat. No.: 10293 (packing)Column: 10' x 1/8" stainless steelOven: 80°CCarrier: helium, 30cc/minDet.: P.E. 900 T.C., 225maInj.: Valco valve, 100 microliters, 140°CFor more information, request Bulletin 722.795-02521. Air (balance)2. Carbon dioxide (approx. 0.1%)3. Water (approx. 2.5%)4. Hydrogen sulfide (approx. 2.5%)0 2 4 86Min4321For more information, request Bulletin 722.795-02530 2 4Min123541. Nitrogen (balance)2. Carbon dioxide (2%)3. Nitrous oxide (3%)4. Water (0.5%)5. Hydrogen sulfide (3%)8 7Sulfur & MercaptansFigure 162. Trace Sulfur GasesPacking: 40/60 Carbopack B HT 100Cat. No.: 20272 (packing)Column: 1.4m x 1/8" OD Teflon (FEP)Cat. No.: 11502-U (general configurationstock column only)Oven: 35°CCarrier: nitrogen, 20mL/minDet.: flame photometricInj.: 0.3mLFigure 161. Light Sulfur Gases, Alkyl Sulfides,and MercaptansPacking: 40/60Carbopack B HT 100Cat. No.: 20272 (packing)Column: 1.4m x 1/8" OD Teflon® (FEP)Cat. No.: 11502-U (general configuration stock column only)Oven: 100°CCarrier: nitrogen, 70mL/minDet.: flame photometricInj.: 0.5mL of syntheticmix1. H2S2. COS3. SO24. CH2SH20Min3142For more information, request Bulletin 722.795-0254For more information, request Bulletin 722.713-080620 6 84 10 12Min12346578 91. SF6, H2S2. SO2, COS3. CH3SH4. Dimethyl sulfide, Ethyl mercaptan5. CS26. Ethyl methyl sulfide, n-Propyl mercaptan7. Methyl disulfide8. Diethyl sulfide9. n-Butyl mercaptan8 880Figure 163. Kraft Pulp Mill Stack GasesPacking: Supelpak™-SColumn: 30" (18" packed) x 1/8" ODTeflon® (FEP)Cat. No.: 12255-U (general configurationstock column only)Oven: 30°C (1 min) to 210°C at 40°C/minCarrier: helium, 30mL/minDet.: flame photometricInj.: 2mLFor more information, request Bulletin 722.1. H2S2. COS3. SO24. CH3SH5. (CH3)2S6. (CH3)2S20 2 4 6Min654321713-0807For more information, request Bulletin 722.713-08051. H2S2. SF63. COS4. SO25. Methyl mercaptan6. Ethyl mercaptan7. Dimethyl sulfide8. CS22 4 6 8 10Min1234576Figure 164. Trace Sulfur Gases with C2 IsomericSulfur CompoundsPacking: GP 60/80 Carbopack B/1.5% XE-60/1.0% H3PO4Cat. No.: 11828 (packing)Column: 6' x 2mm ID glassOven: 50°CCarrier: nitrogen, 35mL/minDet.: flame photometricInj.: 0.25mL8 9Sulfur & MercaptansFigure 165. Alkyl Sulfides and MercaptansPacking: Chromosil 330Column: 8' (6' packed) x 1/8" OD Teflon (FEP)Cat. No.: 11496 (general configuration stock column only)Oven: 65°CCarrier: nitrogen, 20mL/minDet.: flame photometricInj.: 0.5cc of synthetic mixFigure 166. Trace Light Sulfur Gases and C1-C3 MercaptansPacking: Chromosil 330Column: 8' (6' packed) x 1/8" OD Teflon (FEP)Cat. No.: 11496 (general configuration stock column only)Oven: 40°CCarrier: nitrogen, 20mL/minDet.: flame photometricInj.: 0.2mL, approx. 1ppmeach component in nitrogenFor more information, request Bulletin 722.795-0255For more information,request Bulletin 722.713-08041. COS2. H2S3. Methyl mercaptan4. CS25. Ethyl mercaptan6. SO27. Propyl mercaptan20 4 0 0Min1234567Min97864230 2 4 6 8 101. COS + H2S2. CH3SH3. CS24. SO25. Ethyl mercaptan, Dimethyl sulfide6. Propyl mercaptan, Ethyl methyl sulfide7. Diethyl sulfide8. Butyl mercaptan9. Methyl disulfide1 59 0Figure 167. Light Sulfur Gases at Percent ConcentrationsPacking: Chromosil 310Column: 6' x 4mm ID glassCat. No.: 11501 (general configurationstock column only)Oven: 40°CCarrier: helium, 50mL/minDet.: thermal conductivityInj.: 0.3mL of synthetic mixFor more information, request Bulletin 722.794-0844Figure 168. Trace Light Sulfur GasesPacking: Chromosil 310Column: 8' (6' packed) x 1/8" OD Teflon (FEP)Cat. No.: 11501 (general configuration stock column only)Oven: 50°CCarrier: nitrogen, 20mL/minDet.: flame photometricInj.: 0.5mL approx. 1ppm eachcomponent in nitrogenMinFor more information, request Bulletin 722.713-08030 42 6Min43211. COS2. H2S3. CS24. SO20 2 4 65642311. Air2. CO23. COS4. H2S5. CS26. SO29 1Sulfur & MercaptansFor more information, request Bulletin 722.794-0850Figure 169. Mercaptans, Sulfides, and DisulfidesPacking: 12% polyphenyl ether/0.5% H3PO4 on 40/60 Chromosorb TColumn: 36' x 0.085" ID TeflonCat. No.: 11500 (general configuration stock column only)Oven: 50°CCarrier: nitrogen, 80mL/minDet.: FPDInj.: 0.5cc synthetic mixFigure 170. Sulfur GasesPacking: 12% polyphenyl ether/0.5% H3PO4 on 40/60 Chromosorb TColumn: 36' x 0.085" ID TeflonCat. No.: 11500 (general configuration stock column only)Oven: 50°CCarrier: nitrogen, 100mL/minDet.: flame photometric (sens. settings on figure)Inj.: 0.2mL nitrogen approx. 1ppm each analyteFor more information, request Bulletin 722.713-08080 2 4 6 8 10 12 2418 26Min1342567 9 1081. SF62. H2S & COS3. SO24. Methyl mercaptan5. Ethyl mercaptan6. Dimethyl sulfide & CS27. n-Propyl mercaptan8. Methyl ethyl sulfide9. iso-Butyl mercaptan10. n-Butyl mercaptan & Diethyl sulfide0 1 2 3 4 652 x 10-6 4 x 10-8 2 x 10-81 x 10-62345611. Air2. H2S3. SO24. CH3SH5. C2H5SH6. (CH3)2S9 2Figure 171. Cholesterol (Steroids)Packing: 3% SP-2250 on 100/120 SUPELCOPORTCat. No.: 11878 (packing)Column: 3' x 2mm ID glass(stock column available)Oven: 250°CCarrier: nitrogen, 20mL/minFigure 172. Cholesterol (Steroids)Packing: 3% SP-2100 on 80/100 SUPELCOPORTCat. No.: 11987 (packing)Column: 3' x 2mm ID glassOven: 250°CCarrier: nitrogen, 20mL/min795-02351. 5 -Cholestane2. Cholesterol3. 5 -Cholestane-3 -ol4. 5 -Cholestane-3-one795-02361. 5 -Cholestane2. Cholesterol3. 5 -Cholestane-3 -ol4. 5 -Cholestane-3-one0 1 2 43 5 6 7 8 1091 42,3Min0 1 2 3 4 5 6 7 8 9 103412Min9 3Figure 173. Cholesterol (Steroids)Packing: 3% SP-2401 on 100/120 SUPELCOPORTCat. No.: 11978 (packing)Column: 3' x 2mm ID glassOven: 250°CCarrier: nitrogen, 20mL/minFigure 174. Cholesterol (Steroids)Packing: 3% SP-2300 on 100/120 SUPELCOPORTCat. No.: 11888 (packing)Column: 3' x 2mm ID glass(stock column available)Oven: 250°CCarrier: nitrogen, 20mL/minSteroids1. 5 -Cholestane2. Cholesterol3. 5 -Cholestane-3 -ol4. 5 -Cholestane-3-one795-02371. 5 -Cholestane2. Cholesterol3. 5 -Cholestane-3 -ol4. 5 -Cholestane-3-one795-028313240 1 2 3 4 5 6 7 8MinMin10 131211109876543231429 4795-0257795-02581. n-Octacosane2. Androsterone TMS3. Etiocholanolone TMS4. Dehydroepiandrosterone TMS5. 11-Ketoetiocholanolone TMS6. n-Hexatriacontane7. 11 -OH-Etiocholanolone TMSFigure 176. KetosteroidsPacking: 3% OV-225 on 80/100 SUPELCOPORTCat. No.: 11957-U (packing)Column: 6' x 4mm ID glassOven: 250°CCarrier: nitrogen, 20mL/minFigure 175. Plant SterolsPacking: 3% SP-2250 on 100/120 SUPELCOPORTCat. No.: 11878 (packing)Column: 3' x 2mm ID glassOven: 260°CCarrier: nitrogen, 20mL/min0 1 2 3 4 5 6 7 8 9 1110 12Min76543211. Brassicosterol2. Compesterol3. Stigmasterol4. -Sitosterol0 4 6 82 10 12 14 16 Min42139 5Sterols/TriglyceridesFigure 177. TriglyceridesPacking: 1% Dexsil 300 on 100/120 SUPELCOPORTCat. No.: 11972 (packing, 20g/bottle)Column: 18" x 1/8" stainless steelOven: 275°C to 350°C at 8°C/minCarrier: nitrogen, 20mL/minDet.: FID, 350°CInj.: 1µL chloroform containing 1µg each triglyceride, 325°CFigure 178 Pentaerithritol EstersPacking: 1% Dexsil 300 on 100/120 SUPELCOPORTCat. No.: 11972 (packing)Column: 18" x 1/8" stainless steelOven: 125°C to 300°C at 8°C/minCarrier: nitrogen, 20mL/minDet.: 350°C713-09440 2 4 6 8 10 12Min21341. Trilaurin2. Trimyristin3. Tripalmitin4. Tristearin795-02590 2 4 6 8 10 12 14 16 18 20MinC34 to C48 Alcohols9 6Figure 179. Cholesteryl EstersPacking: 1% Dexsil 300 on 100/120 SUPELCOPORTCat. No.: 11972 (packing)Column: 18" x 2mm ID glassOven: 300°C to 350°C at 6°C/minCarrier: nitrogen, 40mL/minDet.: 350°C713-0943For expert answers to your questions,contact our Technical Service DepartmentPhone 800-359-3041 or 814-359-3041FAX 800-359-3044 or 814-359-54680 1 2 3 4 5 6 7Min21341. Laurate2. Myristate3. Palmitate4. StearateAYTFor more information, or current prices, contact your nearest Supelco subsidiary listed below. Toobtain further contact information, visit our website (www.sigma-aldrich.com), see the Supelcocatalog, or contact Supelco, Bellefonte, PA 16823-0048 USA.ARGENTINA · Sigma-Aldrich de Argentina, S.A. · Buenos Aires 1119AUSTRALIA · Sigma-Aldrich Pty. Ltd. · Castle Hill NSW 2154AUSTRIA · Sigma-Aldrich Handels GmbH · A-1110 WienBELGIUM · Sigma-Aldrich N.V./S.A. · B-2880 BornemBRAZIL · Sigma-Aldrich Quimica Brasil Ltda. · 01239-010 São Paulo, SPCANADA · Sigma-Aldrich Canada, Ltd. · 2149 Winston Park Dr., Oakville, ON L6H 6J8CZECH REPUBLIC · Sigma-Aldrich s.r.o.· 186 00 Praha 8DENMARK · Sigma-Aldrich Denmark A/S · DK-2665 Vallensbaek StrandFINLAND · Sigma-Aldrich Finland/YA-Kemia Oy · FIN-00700 HelsinkiFRANCE · Sigma-Aldrich Chimie · 38297 Saint-Quentin-Fallavier CedexGERMANY · Sigma-Aldrich Chemie GmbH · D-82041 DeisenhofenGREECE · Sigma-Aldrich (o.m.) 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