MedChemExpress - Model Methyl Orange -547-58-0

Methyl Orange is a soluble azo dye commonly used as an acid-base indicator and for staining cells and tissue sections, as well as for dyeing textiles. Methyl Orange appears red at a pH of 3.1 and changes to bright yellow as the pH increases to 4.4. Methyl Orange (500 mg/L) exhibits cytotoxicity and can cause DNA damage[1][2][3].

Methyl Orange

MCE China：Methyl Orange

Brand：MedChemExpress (MCE)

Cat. No.HY-118907

CAS：547-58-0

Purity：99.41%

Storage：Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month

Shipping：Room temperature in continental US; may vary elsewhere.

Description：Methyl Orange is a soluble azo dye commonly used as an acid-base indicator and for staining cells and tissue sections, as well as for dyeing textiles. Methyl Orange appears red at a pH of 3.1 and changes to bright yellow as the pH increases to 4.4. Methyl Orange (500 mg/L) exhibits cytotoxicity and can cause DNA damage.

In Vivo：1. Acid-Base Indicator[2] Methyl Orange exhibits a distinct color change at different pH values: pH pH 3.1 - 4.4: Orange pH > 4.4: Bright Yellow 2. Methyl Orange Vivo Staining Effects and Toxicity Studies[3] 2.1 Preparation of Working Solution Dissolve Methyl Orange in Hoagland nutrient solution to a concentration of 2.0 mmol/L. 2.2 Vivo Staining (1).Select healthy, uniformly sized, well-rooted, and pest-free Spider Plant cuttings and transplant them into Hoagland nutrient solution containing 2.0 mmol/L Methyl Orange at pH 6.5. Change the nutrient solution every 3 days. (2).On days 20, 40, and 60 of treatment, randomly select 10 mature, healthy, pest-free leaves from each plant and measure the leaf color parameters in the middle of the leaf surface using an NC-9801 handheld colorimeter. (3).On day 60 of treatment, measure the biomass and root-to-shoot ratio, where root-to-shoot ratio = (fresh weight of underground part / fresh weight of above-ground part) × 100%. (4).Starting from day 10 of treatment, measure leaf nutrient content every 10 days, including soluble sugars, starch, soluble proteins, total nitrogen, chlorophyll, and carotenoids. 1.3 Data Analysis Perform significance analysis using SPSS 22.0 software to determine the staining effect of Methyl Orange on Spider Plants and its impact on their biological activity.

References：

[1]. Ahmad M, et al. Photocatalytic degradation of methyl orange from wastewater using a newly developed Fe-Cu-Zn-ZSM-5 catalyst. Environ Sci Pollut Res Int. 2020;27(21):26239-26248. [Content Brief]

[2]. Sandberg, et al. Kinetics of acid dissociation-ion recombination of aqueous methyl orange. The Journal of Physical Chemistry. 1972, 76 (26): 4023–4025. of aqueous methyl orange. The Journal of Physical Chemistry. 1972

[3]. 张永福, 等. 活体染色对银边吊兰叶面着色及植株碳氮营养的影响[J]. 西南林业大学学报, 2017, 37(5): 80-87.

[4]. Alaguprathana M, et al. Cytogenotoxicity assessment in Allium cepa roots exposed to methyl orange treated with Oedogonium subplagiostomum AP1. Environ Res. 2022 Oct;213:113612. [Content Brief]

Brand introduction:
•   MCE (MedChemExpress) has a global exclusive compound library of more than 200 kinds, and we are committed to providing the most comprehensive range of high-quality small molecule active compounds for scientific research customers around the world；
•   More than 50,000 highly selective inhibitors and agonists are involved in various popular signaling pathways and disease areas；
•   The products cover a variety of recombinant proteins, peptides, commonly used kits, more PROTAC, ADC and other characteristic products, widely used in new drug research and development, life science and other scientific research projects；
•   Provide virtual screening, ion channel screening, metabolomics analysis detection analysis, drug screening and other professional technical services；
•   It has a professional experimental center and strict quality control and verification system；
•   Provide LC/MS, NMR, HPLC, chiral analysis, elemental analysis and other quality inspection reports to ensure the high purity and high quality of products；
•   The biological activity of the products has been verified by the experiments of customers in various countries；
•   A variety of top journals such as Nature, Cell, Science and pharmaceutical patents have included the scientific research results of MCE customers；
•   Our professional team tracks the latest pharmaceutical and life science research and provides you with the latest active compounds in the world；
•   It has established long-term cooperation with the world's major pharmaceutical companies and well-known scientific research institutions。

MedChemExpress - Model Methyl Orange -547-58-0

MCE products for research use only. We do not sell...

Methyl Orange