MedChemExpress - Model Biotin -58-85-5

Biotin, vitamin B7 and serves as a coenzyme for five carboxylases in humans, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids[1][2][3].

Biotin

MCE China：Biotin

Brand：MedChemExpress (MCE)

Cat. No.HY-B0511

CAS：58-85-5

Synonyms：Vitamin B7; Vitamin H; D-Biotin

Purity：99.58%

Storage：Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month

Shipping：Room temperature in continental US; may vary elsewhere.

Description：Biotin, vitamin B7 and serves as a coenzyme for five carboxylases in humans, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids.

In Vitro：Biotin exhibits higher affinity on breast cancer (T47D) cells over normal mammary epithelial (MCF-12A) cells, with Kms of 9.24 μM and 53.1 μM, respecetively[4]. Biotin (0.09-100 μM; 0-70 min) is dose-dependently uptake by T47D cells with Vmax of 27.34 pmol/mg protein/min[4]. Biotin (1-1000 nM; 24 h) exerts function in the recovery of 7β-OHC (50 µM)-induced cell death and reduces cell adhesion[5].

In Vivo：Biotin (15 mg/kg/d; p.o.; 12 d) improves nephrotoxicity in streptozotocin-induced (150 mg/kg; i.p.) diabetic mice[6]. Biotin (0.012 mg/kg/d; p.o.; 70 d) deficiency impaires the immune function of the head kidney, spleen and skin in fish with inadequate administration dose[7].

IC50 & Target：Microbial Metabolite Human Endogenous Metabolite

References：

[1]. Zempleni, et al. Biotin biochemistry and human requirements. J Nutr Biochem, 1999. 10(3): p. 128-38. [Content Brief]

[2]. Mock, D.M.. Biotin status: which are valid indicators and how do we know? J Nutr, 1999. 129(2S Suppl): p. 498S-503S. [Content Brief]

[3]. Zempleni J, et al. Biotin. Biofactors. 2009 Jan-Feb;35(1):36-46. [Content Brief]

[4]. Vadlapudi AD, et al. Biotin uptake by T47D breast cancer cells: functional and molecular evidence of sodium-dependent multivitamin transporter (SMVT). Int J Pharm. 2013 Jan 30;441(1-2):535-43. [Content Brief]

[5]. Sghaier R, et al. Biotin attenuation of oxidative stress, mitochondrial dysfunction, lipid metabolism alteration and 7β-hydroxycholesterol-induced cell death in 158N murine oligodendrocytes. Free Radic Res. 2019 May;53(5):535-561. [Content Brief]

[6]. Aldahmash BA, et al. Biotin amelioration of nephrotoxicity in streptozotocin-induced diabetic mice. Saudi J Biol Sci. 2015 Sep;22(5):564-9. [Content Brief]

[7]. He P, et al. Dietary biotin deficiency decreased growth performance and impaired the immune function of the head kidney, spleen and skin in on-growing grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol. 2020 Feb;97:216-234. [Content Brief]

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MedChemExpress - Model Biotin -58-85-5

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Biotin