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MedChemExpress - Model TPCK-treated Trypsin -9002-07-7
MCE products for research use only. We do not sell to patients.
TPCK-treated Trypsin
MCE China:TPCK-treated Trypsin
Brand:MedChemExpress (MCE)
Cat. No.HY-129047C
CAS:9002-07-7
Storage:Please store the product under the recommended conditions in the Certificate of Analysis.
Shipping:Room temperature in continental US; may vary elsewhere.
Description:TPCK-treated Trypsin is a trypsin whose activity is inhibited by tosyl phenylalanyl chloromethyl ketone. TPCK-treated Trypsin reduces autolysis and nonspecific proteolysis during experiments, exhibits stability in storage and handling. TPCK-treated trypsin can be used in proteomics research.
In Vitro:Product InformationThis product is derived from bovine pancreas. After chromatographic purification, it is treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit the activity of proteases. The theoretical molecular weight is 23.3 kDa, and the optimal pH is 7.5-8.5. It can be used for protein analysis, such as protein mass spectrometry, sequencing, and peptide mapping analysis. Instructions (for reference only)This product can be directly used for protein enzymatic cleavage. The recommended enzymatic cleavage ratio is 1:50-1:100 (w/w); Enzymatic cleavage overnight at 37℃.
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References:
[1]. Shuford CM, et al., Cheaper, faster, simpler trypsin digestion for high-throughput targeted protein quantification. J Mass Spectrom Adv Clin Lab. 2023 Nov 23;30:74-82. [Content Brief]
Brand introduction:
• MCE (MedChemExpress) has a global exclusive compound library of more than 200 kinds, and we are committed to providing the most comprehensive range of high-quality small molecule active compounds for scientific research customers around the world;
• More than 50,000 highly selective inhibitors and agonists are involved in various popular signaling pathways and disease areas;
• The products cover a variety of recombinant proteins, peptides, commonly used kits, more PROTAC, ADC and other characteristic products, widely used in new drug research and development, life science and other scientific research projects;
• Provide virtual screening, ion channel screening, metabolomics analysis detection analysis, drug screening and other professional technical services;
• It has a professional experimental center and strict quality control and verification system;
• Provide LC/MS, NMR, HPLC, chiral analysis, elemental analysis and other quality inspection reports to ensure the high purity and high quality of products;
• The biological activity of the products has been verified by the experiments of customers in various countries;
• A variety of top journals such as Nature, Cell, Science and pharmaceutical patents have included the scientific research results of MCE customers;
• Our professional team tracks the latest pharmaceutical and life science research and provides you with the latest active compounds in the world;
• It has established long-term cooperation with the world's major pharmaceutical companies and well-known scientific research institutions。
