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MedChemExpressModel Subtilisin -9014-01-1

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Subtilisin, or rubinase, is a bacterial serine protease. Subtilisin can be used as a detergent additive with thermal stability, pH tolerance, and calcium dependent stability[1][2].
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Subtilisin

MCE China:Subtilisin

Brand:MedChemExpress (MCE)

Cat. No.HY-P2729

CAS:9014-01-1

Synonyms:EC 3.4.21.14

Storage:Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping:Room temperature in continental US; may vary elsewhere.

Description:Subtilisin, or rubinase, is a bacterial serine protease. Subtilisin can be used as a detergent additive with thermal stability, pH tolerance, and calcium dependent stability.

In Vitro:Product Information It is a proteolytic enzyme made by deep fermentation, extraction and purification of Bacillus subtilis selected by protoplast mutagenesis method. It can hydrolyze the peptide chain of protein molecules to generate polypeptides or amino acids. It can also catalyze the synthesis of polypeptides in organic solvents. This product contains stabilizers. Effective temperature range: 20 - 60℃, the optimum temperature is between 35-45℃. Effective pH range: 6.0 - 11.0, the optimum PH range is between 9.5-10.5. Activators: Ca2+, Mg2+, Mn2+, Zn2+, Fe2+. Inhibitors: Reagents acting on serine such as sulfonyl halides. InstructionsSoluble in 100 mg/mL ddH2O, avoid repeated freezing and thawing.Or dissolve according to specific experimental references.

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References:

[1]. Philipp M, et al. Kinetics of subtilisin and thiolsubtilisin. Mol Cell Biochem. 1983;51(1):5-32.  [Content Brief]

[2]. Azrin NAM, et al. Versatility of subtilisin: A review on structure, characteristics, and applications. Biotechnol Appl Biochem. 2022 Dec;69(6):2599-2616.  [Content Brief]

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