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REAGEN - Model RNF91005 -Ascorbic Acid Assay elisa kit
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REAGEN™ Ascorbic Acid Assay Kit is a cuvette-based enzymatic assay for the determination of Ascorbic Acid across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Ascorbic Acid directly from food samples.
The unique features of the kit are:
High sensitivity and low detection limit (0.6 ppm)
High reproducibility
A rapid (25 minute), robust enzyme-based assay.
Procedure Overview
REAGEN™ Ascorbic Acid Assay Kit uses a specific visible, colorimetric reaction to detect ascorbic acid in food samples: ascorbic acid first reduces an electron carrier (PMS). This compound then reduces MTT to produce a colored MTT formazan product. This conversion, measured at 578 nm, is proportional to the level of ascorbic acids in the sample.
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