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REAGENModel RNF91005 -Ascorbic Acid Assay elisa kit

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Contact: [email protected] / [email protected] REAGEN™ Ascorbic Acid Assay Kit is a cuvette-based enzymatic assay for the determination of Ascorbic Acid across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Ascorbic Acid directly from food samples. The unique features of the kit are: High sensitivity and low detection limit (0.6 ppm) High reproducibility A rapid (25 minute), robust enzyme-based assay. Procedure Overview REAGEN™ Ascorbic Acid Assay Kit uses a specific visible, colorimetric reaction to detect ascorbic acid in food samples: ascorbic acid first reduces an electron carrier (PMS). This compound then reduces MTT to produce a colored MTT formazan product. This conversion, measured at 578 nm, is proportional to the level of ascorbic acids in the sample.