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ReverTra Ace -a- - High Efficient Reverse Transcription Kit
ReverTra Ace-α- is an efficient and convenient kit to synthesize high quality cDNA. This kit contains the highly efficient reverse transcriptase "ReverTra Ace®", as well as other components optimized for the synthesis of long cDNAs suitable for RT-PCR. ReverTra Ace® is an M-MLV reverse transcriptase that has been improved by point mutation technology. ReverTra Ace® has two mutations in the polymerase and RNase H domains.
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Features
- Contains all components for reverse transcription.
- Enables the synthesis of ≥ 14 kb cDNA.
Applications
- cDNA synthesis
- RT-PCR
Storage condition
- Store at -20°C
- ReverTra Ace® : 100 µl
- 5xRT buffer (contains 25 mM Mg2+) : 400 µl
- Rnase inhibitor (10 U/µl) : 100 µl
- dNTPs mixture (10 mM) : 200 µl
- RNase-free H2O : 1200 µl
- Oligo (dT)20 (10 pmol/µl) : 100 µl
- Random primers (25 pmol/µl) : 100 µl
- Control Primer F (10 pmol/µl) : 50 µl
- Control Primer R (10 pmol/µl) : 50 µl
- Positive control RNA (105 copies/µl) : 50 µl