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Minerva Biolabs - PCR Quantification Standards Kit
Our PCR Quantification Standards contain genomic DNA extracted from defined microorganisms, at low culture passage number. The DNA starting material is analyzed with standardized methods to determine the DNA’s integrity and quantity. Only pure and intact DNA showing a > 10 kb band in an agarose gel and an OD260/280 > 1.8 is used for the manufacture of these products. The DNA is partially sequenced to confirm the species’ identity.
Defined DNA standards for quantitative determination of microbial organisms in PCR-based detection methods.
- Broad range of organisms available: bacteria, yeasts, and fungi
- Contains a defined number of genome copies/vial (1x10^6 or 1x10^8)
- Suitable for standard curves, quantification, method verification, and reproducible PCR results
- Non-infectious
Recommended Use
PCR Quantification Standards contain a defined number of genome copies of a particular microorganism. Therefore, our standards are perfectly suitable to generate DNA standard curves in qPCR. In addition, standards are required for validating the specificity and testing the sensitivity of PCR assays in the context of quality control and EP 2.6.7 compliant-testing.
Content
- 1 vial with 1x108 genomes (lyophilized)
- 3 vials with rehydration buffer (10 mM, pH 8.5)
Shelf Life and Storage
The components must be stored between +2 and +8 °C (Shipping at ambient temperature).