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Model RT-20 -Quanty Usutu System
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The quanty Usutu system is a quantitative test that allows the RNA amplification, by means of Real-Time PCR, of 3’UTR region of Usutu Virus (USUV). The Procedure allows the detection and quantification of the RNA target by means a One step retro-amplification reaction. The analysis of the results is obtained using a Real-Time PCR analyzer (thermal cycler integrated with a system for fluorescence detection and a dedicated software).
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Target:
- 3’UTR region of Usutu Virus (USUV)
- Internal Control (IC)
Standard for quantification:
- synthetic RNA corresponding to 3’UTR region of USUV 100.000 cps/µL
- synthetic RNA corresponding to 3’UTR region of USUV 10.000 cps/µL
- synthetic RNA corresponding to 3’UTR region of USUV 1.000 cps/µL
- synthetic RNA corresponding to 3’UTR region of USUV 100 cps/µL
Diagnostic samples:
- Serum
- Urine
Real-Time PCR instruments:
- Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
- Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)
-
LightCycler® 480 Real-Time PCR System (Roche)
RNA Extraction:
Manual Extraction with:
- QIAmp Viral RNA Mini Kit (QIAGEN)
Automatic Extraction with:
- EZ1 Advanced XL (QIAGEN)
- QIAsymphony SP (QIAGEN)