BioLegend - Spectral Cytometry Analyzer

Spectral cytometers capture the full spectrophotometric profile of the fluorophores across all lasers. The fluors’ profiles are captured in 10-30 nm segments across the emission range to accurately unmix the fluorophores. This allows fluors that normally cannot be unmixed on a conventional cytometer (e.g. Pacific Blue™ and Brilliant Violet 421™) to be used together on an instrument like Cytek’s Aurora. As such, this web tool is dedicated to displaying the emission spectra of fluorophores based on Cytek’s Aurora spectral cytometers.