HTX - Model TM -Tissue Maldi Sample Preparation Sprayer System
Our patented TM-Sprayer™ï¸ MALDI Sample Preparation System is designed to provide the highest quality matrix deposition on 2D biological samples and can be integrated with any MALDI mass spectrometer platform.
CONTROLLED DRYING TIME
The HTX TM-Sprayer allows users to adjust both gas flow rate and nozzle velocity to control drying time, preventing analyte delocalization and the formation of large matrix crystals, known to create "hot spots" on MALDI images.
PATENTED HEATED NOZZLE
HTX is the only sample preparation system available that allows users to heat the matrix solution as it is being applied to their sample. By increasing the temperature of the matrix solution, absorption of matrix into the sample is increased, resulting in greater MALDI image resolution.
EFFICIENCY AND PRECISION
The HTX TM-Sprayer`s easy-to-use software interface enables the user to quickly adjust sample parameters for rapid preparation time of < 15 minutes for a single glass slide.
The HTX TM-Sprayer™ is an easy-to-use, versatile spraying system that provides an automated process for Sample Preparation in Mass Spectrometry Imaging. !e patented spray technology of the TM-Sprayer™ guarantees a very "ne, uniform and consistent matrix coating crucial for high-resolution imaging and relative quanti"cation of analytes. !e new HTX Technologies’ spray nozzle, featured in the next generation TM-Sprayer, creates a "ne solution mist that can be deposited in a precise and adjustable pattern over all or part of any MALDI plate. Spray characteristics (wet or dry) are easily adjustable via the intuitive operator interface. Users can create and save methods for reproducible operation.
Key Characteristics
- Patented technology providing very small matrix droplets (
- High #ow rate and fast sample prep (10 to 20 minutes per plate )
- Highly consistent matrix deposition across entire sample area (+/- 3% by weight)
- Unique use of temperature and nitrogen #ow to control evaporation rate and matrix crystal formation
- Validated protocols for most matrices (e.g.: SA, CHCA, DHB)
- Validated protocols for Trypsin digestion
- Continuous matrix coverage as needed for high-resolution imaging
- Rugged operation and easy clean-up
Addressing the Matrix Deposition Challenge
The main challenge when preparing samples for MALDI Mass Spectrometry Imaging is to balance the positive e$ects of the matrix solution penetrating the tissue and co-crystallizing with the analytes, and the negative e$ects of analytes delocalization.
Where other available systems can only vary matrix solution volume and drying time between depositions, the TM-Sprayer adds two new dimensions by providing temperature control of the drying gas (nitrogen) and nozzle velocity as additional control variables. Having access to these additional control parameters enables the user to better optimize extraction and signal intensity without compromising resolution.
Automated Matrix Deposition
Because the base concept of the TM-Sprayer is similar to that of the airbrush (mixing air and liquid #ow to create a "ne spray), users "nd it very easy to operate the TM-Sprayer, while also enjoying the many bene"ts of automation:
- Reduced exposure to matrix solution
- High mechanical reproducibility