Assay Development Services

• Target Protein Sourcing/Production - If the target protein is available from the customer or vendor, we will test it for for purity and functional activity; if not, we will produce it using the most efficient and cost-effective expression and purification strategy.
• Assay Optimization with Target Protein - We will determine the optimal buffer composition, enzyme concentration, and reaction time & optimize detection reagents to produce maximal signal under initial velocity conditions.
• Assay Validation - Depending on customer needs, validation could include dose responses with control inhibitors, determination of kinetic parameteres, pilot screens with target libraries or interference screens.

Example Assay Optimization with Human cGAS
BellBrook scientists performed a detection of purified, full-length human cGAS with the Transcreener cGAMP FP Assay. Linear response is shown from polarization data converted to cGAMP using a standard curve.

Example Inhibitor Screening & Profiling With Human cGAS
BellBrook scientists performed an inhibitor screening & inhibitor potency profiling of cGAS using the Transcreener cGAMP cGAS Assay.

Screen of 1600 compounds. cGAS was used at 10 nM, compounds at 10 μM. Z = 0.62, Z` = 0.7 illustrate a robust screening assay. Hits were determined to be anything outside of three standard deviations from the mean.

Confirmation of a screening hit using the Transcreener cGAMP cGAS Assay in the FP format. Follow-up assays to triage screening hits can be used to selective bonafide inhibitors for advancement into medicinal chemistry/SAR.