Huygens Spinning Disk Deconvolution Software
Huygens Widefield Deconvolution Software employs advanced algorithms to enhance signal, contrast, and resolution of microscopy images. It corrects for spherical aberration and noise, making it suitable for various microscopy types including widefield, confocal, and STED. The software utilizes Maximum Likelihood Estimation algorithms to provide true deconvolution and reliable quantification. It is compatible with all microscope brands and is particularly effective in deep tissue imaging. Huygens also includes a bleaching corrector for retrieving clear images from low-signal raw data. Additional tools like PSF Distiller and Colocalization Analyzer complement the software, providing extensive analytical capabilities. The software is used in various research fields, including cell biology and genetics, as evidenced by scientific publications.
Many researchers use Huygens to routinely correct for noise, blur, and low signal within Spinning Disk microscopy images, including Yokogawa SoRa data. The Spinning Disk deconvolution option of Huygens is unique in that it includes a pinhole spacing parameter that considers the crossover of signal between neighboring pinholes on the disk which is detrimental to the quality of the raw image data. Huygens deconvolution significantly increases the quality, contrast, and resolution in xyz images. As a result, images will be easier to visualize and subsequent analysis is more reliable. Because image signal can increase manifold, Huygens even allows you to image faster at lower light dose, which minimizes bleaching and phototoxicity.
SoRa Microscopes
SoRa microscopes are great for providing fast imaging of live samples with a high resolution. Huygens deconvolution further improves the quality of your data in each of these dimensions, using a PSF that is optimized for the SoRa principle (optical reassignment).
high resolution The inventors of SoRa, the Yokogawa Electric Corporation, already recognized the potential of using deconvolution, preferring Huygens Deconvolution on biological data over alternatives, and demonstrating a lateral resolution of 113 nanometer with Huygens in their paper1.
compensate chromatic aberrations The SoRa system uses a microlens array to achieve a higher resolution, but this comes at the cost of having higher chromatic aberrations as the microlenses are not achromatic1. Huygens can fully compensate for this inherent drawback using the chromatic aberration corrector.
even longer live experiments SoRa benefits from the signal-to-noise enhancement of deconvolution. This means that the acquisition time can be decreased without loss of information in the final image (See: Does deconvolution work on noisy data?). For live samples, this can be leveraged to image longer or faster without risk of photobleaching or phototoxicity due to prolonged light exposure.