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Huygens Widefield Deconvolution Software
The Huygens Widefield Deconvolution Software is designed to enhance signal, contrast, and resolution in various microscopy applications through the use of advanced deconvolution algorithms. The software supports a range of microscopy techniques including widefield, confocal, spinning disk, multi-photon, STED, light sheet, and array detector systems, among others. It addresses issues such as noise, spherical aberration, and bleaching, thereby providing reliable quantification and improved imaging quality. The deconvolution process uses Maximum Likelihood Estimation algorithms to correct distortions and improve clarity, making it suitable for deep tissue imaging. Huygens software is compatible with all microscope brands and capable of creating clear images from low-signal raw data, which is critical for detailed cellular and molecular visualizations in genetic and cell biology research.
One of the main issues with widefield imaging is out-of-focus blur, which typically hinders 3D imaging and clear object recognition and segmentation. Luckily, Huygens true deconvolution can use this fluorescence to increase image resolution and specific object signal. This improvement, together with Huygens' well-known spherical aberration correction, offers true image detail, even deep within thick samples. Brightfield images also greatly benefit from Huygens Widefield & Brightfield Microscope deconvolution.
3D widefield imaging
All fluorescence microscopy images suffer from out-of-focus light, which hinders clear 3D rendering of the object. This light is especially problematic when there is Spherical Aberration due to a mismatch between the refractive indices of the sample and the lens medium. The image quality then gets progressively worse as you move deeper within the sample. With Huygens, deconvolution is done in a depth-dependent manner such that the resulting image is consistent in quality over its entire depth range. A reliable 3D rendering can then be made and analyzed.
Bleaching correction
The Huygens Bleaching Corrector can correct for photobleaching and illumination instability in the depth and time dimension. The resulting image is consistent in quality over a larger depth or time range, as compared to the raw input image.
In addition, clear images can be retrieved from low-signal raw data since Huygens Deconvolution removes noise while improving contrast. Thus, lower light intensity can be used during imaging, allowing for deeper and longer imaging with less bleaching.