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Atp Concentration Product Applications
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The luc gene coding for firefly luciferase is a reliable in vivo or in vitro reporter gene. ATP (adenosine triphosphate) participates in a large number of these reactions. Using the firefly luciferase reaction emitting a light proportional to the ATP concentration makes it possible to follow the reactions in a simple and highly sensitive way. Calibration of the assays with our certified liquid-stable ATP standard provides robust and reliable ...
ByBioThema AB based in Handen, SWEDEN
The intracellular ATP concentration is similar in different cell types. This means that bacterial cells contain 1-2 attomoles per cell while animal and plant cells contain 100 to 10 000 times as much ATP. Extracellular ATP may be degraded by adding ATP consuming enzymes or by physical separation. ...
ByBioThema AB based in Handen, SWEDEN
Methodology: All ATP producing and ATP degrading enzymatic reactions can be continuously monitored by measuring the light from firefly luciferase/luciferin being present in the reaction mixture. Examples include protein kinases67, adenylate kinase14, pyruvate kinase6, pyruvate phosphate dikinase, glycerol kinase43, 44, creatine kinase isoenzymes8, 10, 18, 22, ATPases14, ATP synthetases49, aminoacyl tRNA synthetase. Substrates to such enzymes can be measured kinetically or with endpoint assays. Examples include ...
ByBioThema AB based in Handen, SWEDEN