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Cell Count Product Applications In Germany
5 applications found
Methodology: All living cells contain ATP and when the cell dies ATP is degraded by intracellular enzymes to ADP and finally AMP. The intracellular ATP concentration is similar in different cell types. This means that bacterial cells contain 1-2 attomoles per cell while animal and plant cells contain 100 to 10 000 times as much ATP. Extracellular ATP may be degraded by adding ATP consuming enzymes or by physical separation. Determination of intracellular ATP can then be used to estimate biomass. If the amount of ...
ByBioThema AB based in Handen, SWEDEN
In cleaning industry luciferase based assays of ATP and ATP+AMP is used for hygiene control in food and beverage industry, restaurants, hospitals and pharma industry. It is also used to develop improved cleaning tools and procedures and for education of cleaning personnel. The advantages area that an objective result is obtained, that seller and purchaser can agree on a measurable degree of cleanliness and that the cleaning personnel can perform the test in less than one minute using a portable instrument ...
ByBioThema AB based in Handen, SWEDEN
In biochemistry, molecular biology and biology there is a need to measure cells, enzymes, metabolites and DNA/RNA. The luc gene coding for firefly luciferase is a reliable in vivo or in vitro reporter gene. ATP (adenosine triphosphate) participates in a large number of these reactions. Using the firefly luciferase reaction emitting a light proportional to the ATP concentration makes it possible to follow the reactions in a simple and highly sensitive way. Calibration of the assays with our certified liquid-stable ...
ByBioThema AB based in Handen, SWEDEN
Clinical diagnostics as e.g. measurement of bacteria in urine with a luciferase based assay of bacterial ATP can be used in the diagnosis of urinary tract infection (UTI). Screening for Duchenne’s Muscular Dystrophy (DMD) has been performed in 528,410 newborn boys in a German study. Luciferase based assays of ATP and ATP+AMP is more and more used to control hospital hygiene. Please have a look at the application listing below to navigate to the product that matches your need. ...
ByBioThema AB based in Handen, SWEDEN
In the development of new drugs high throughput screening (HTS) is often used to screen large libraries of compounds for inhibitors or stimulators of various reactions. The firefly luciferase reaction can be performed reliably in a small reaction volume and is therefore highly suitable for HTS. Our Protein Kinase Kit RR can be used to measure not only protein kinase, but also all ATPases and aminoacyl tRNA synthetases. Growth inhibition from e.g. antibiotics can be measured. Gene expression can be monitored. ...
ByBioThema AB based in Handen, SWEDEN