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Agarose Gel Articles & Analysis
10 articles found
Then the antibody/antigen complex is extracted from the sample with agarose gel coupled with bacterial protein A/G.IP mainly captures target proteins and their interacting proteins selectively. ...
Two different sizes of DNA fragments including longer insert (2-2.5 kb) and short insert (0.5-1.2 kb) are selected from the agarose gel. While the long inserts are cloned in phage or socmid vectors, the short inserts are cloned in plasmid vectors. ...
Electrophoresis means that a charged sample (protein, nucleotide, etc.) is placed in an inert support medium (such as paper, cellulose acetate, agarose gel, polyacrylamide gel, etc.) and with the effect of an electric field, would move to the corresponding electrode direction at their respective speeds, thus different components are separated ...
Therefore, GelGreen is compatible with a 254nm UV transilluminator or a gel reader equipped with visible light excitation (such as a 488nm laser-based gel scanner or a dark reader). ...
Results indicated that conventional PCR visualized on 1% agarose gel was able to detect as low as 0.02 pg genomic DNA and 5 cells, while real-time PCR could detect 0.01 pg genomic DNA and 2 cells of E. histolytica. ...
While developing the SNP techniques we tested several platforms, including LI-COR, nondenaturing polyacrylamide, and agarose gel detection. The agarose gel system was used for SNP genetic mapping in two common bean mapping populations, showing that heteroduplex cleavage is a useful technique for increasing molecular marker number ...
In this condition, supports are able to bind 4 mg of HEP-1 per mL of gel. In order to test the activity of this new immobilised derivative, it was used in the affinity purification of the recombinant Hepatitis B surface antigen. We made a comparison between immunosorbents activated by the CNBr and aldehyde–agarose methods and subsequently studied the recovery ...
The apoptosis of the thymocytes was determined by measuring nuclear condensation using propidium iodide (PI) and DNA fragmentation by gel electrophoresis. The viability of the thymocytes was determined by the trypan blue exclusion method which was found to gradually decrease after incubation of irradiated cells for 7 h. ...
We evaluated four different DNA purification methods (silica-based DNA binding, agarose gel electrophoresis, ammonium acetate precipitation, and Sephadex G-200 gel filtration) for DNA recovery and removal of PCR inhibitors from crude extracts. ...
We evaluated four different DNA purification methods (silica-based DNA binding, agarose gel electrophoresis, ammonium acetate precipitation, and Sephadex G-200 gel filtration) for DNA recovery and removal of PCR inhibitors from crude extracts. ...