Ames Assay Articles & Analysis
8 articles found
The aim of the present study was to explore and quantify the inhibition of the P‐gp efflux pumps by several ubiquitous aquatic contaminants. The calcein‐AM assay commonly used in pharmacological research was established with P‐gp over‐expressing Madin‐Darby Canine Kidney cells (MDCKII‐MDR1) in a 96‐well plate, avoiding extra washing, centrifugation and lysis ...
Genotoxicity was tested in vitro using the Ames and Comet assay. All samples showed negative results. Samples were also tested in in vivo genotoxicity tests in Eastern mudminnow fish (Umbra pygmaea) by a sister chromatid exchange (SCE) and a Comet assay. No significant increases in SCEs were observed, but gill cells isolated from fish exposed to ...
Assimilable organic carbon (AOC) tests and an Ames assay using Salmonella typhimurium TA98 and TA100 on such a proposed integrated system were also performed to determine re-growth potential of bacteria and genotoxicity, respectively. ...
Fractions 7 to 15 of all four extracts were consistently more potent in both the Ames fluctuation and H295R assays. Much of this toxicity could be attributed to polycyclic aromatic hydrocarbons, sterols, and in fraction 7‐naphthoic acids. ...
This study assessed genotoxic risk associated with the possible release of genotoxic compounds into water from PET bottles during SODIS, using the Ames fluctuation test. Negative genotoxicity results were obtained for water samples that had been in PET bottles and exposed to normal SODIS conditions (strong natural sunlight) over 6 months. ...
The mutagenicity and the mutagen formation potential (MFP) of the identified by-products were evaluated by the Ames assay (preincubation method) using Salmonella typhimurium TA100 without exogenous activation by S9 mix (TA100-S9). ...
In our experimental conditions, only two samples out of seven raw and four processed water extracts (Amberlite XAD-2 concentration) gave mutagenic effects using the Salmonella typhimurium/microsome assay (Ames test).Keywords: mutagenicity, surface water, organic micropollutants, drinking water, mutagenic activity, genotoxic compounds, environmental pollution, ...
Changes in isolate character during SAT were established via biodegradability (batch test), specific ultraviolet light absorbance (SUVA), trihalomethane formation potential (THMFP), and Ames mutagenicity assays. The dissolved organic carbon (DOC) concentration decreased by >90% during SAT. ...