Automatic Hplc Analyzer Articles & Analysis
10 articles found
The detector collects the separated ions and determines the M/Z value of each ion. The mass analyzer can analyze the M/Z of each peptide segment and obtain the M/Z spectrum of all peptide segments of the protein, which is the primary mass spectrum peak diagram of the protein. ...
Testing the content of collagen often involves biochemical analytical methods, which can be either direct or indirect. Here are several common techniques for testing collagen content:1. Hydroxyproline Content Determination(1) Collagen is rich in hydroxyproline, thus, the collagen content can be indirectly calculated by determining the hydroxyproline content of a sample.(2) This method typically ...
Protein synthesis starts from the N-terminus and the composition of the N-terminal sequence of the protein affects the overall biological function of the protein. For example, the N-terminal sequence affects the half-life of the protein and is related to the location of the protein subcellular organelle. Analysis of protein N-terminal sequencing helps in the analysis of protein's higher structure ...
Recombinant Protein Glycosylation Analysis is a technique used for the detection and analysis of the glycan structure and composition of recombinant proteins. Glycosylation is a common post-translational modification of proteins, which significantly impacts the stability, activity, and cellular interactions of proteins. Recombinant Protein Glycosylation Analysis is particularly important for the ...
N-terminal sequencing is a technique used to determine the N-terminal (amino terminal) amino acid sequence of a protein or peptide. This technique is useful in the fields of biochemistry and molecular biology, especially when researchers want to confirm the starting position of a protein or determine whether a particular protein has undergone a specific modification.Common N-terminal sequencing ...
Electrochemical detection (ECD) for HPLC or UHPLC / UPLC is an extremely selective and sensitive detection technique that is applied in several analyses such as the neurotransmitters dopamine, serotonin and noradrenalin, or analytes such as 5HIAA, catecholamines and metanephrines. ECD has an enormous linear dynamic range of more than 6 orders of magnitude. This means that concentrations can be ...
The dual-layer LpDNPH sorbent tube is an air sampling device designed for sampling carbonyls (e.g. formaldehyde) in ambient, indoor, and industrial atmospheres. Carbonyls are trapped on our high-purity silica gel adsorbent coated with 2,4-dinitrophenylhydrazine (2,4-DNPH) where they are converted to stable hydrazone derivatives. The derivatives are eluted from the tube in acetonitrile and ...
Application Report 225 Phenolic and Benzyl Compounds Using Ascentis™ C18 This application demonstrates the suitability of Ascentis C18 for the efficient separation of phenolic and benzyl compounds by HPLC. Compounds analyzed include: Uracil, Phloroglucinol, Pyrogallol, Benzamide, Resorcinol, Catechol, Phenol, and Nitrobenzene. ...
Perfluorinated Compounds (PFCs) are fluorocarbons used in many commercial and domestic applications. They make materials stain, oil, and water resistant. They are persitent pollutants and are therefore monitored worldwide. Twelve PFC congeners were extracted from soil and sewage sludge samples using the SpeedExtractor E-916 and an ASE 200 system (Dionex). The PFC congeners were analyzed by ...
ByBUCHI
Recovery rates for polar analytes, including carbamate and thiourea pesticides, are higher and less variable with ENVI-Carb carbon-based solid phase extraction tubes than with C8- or C18- silica-based tubes. Independent investigators found ENVI-Carb tubes to be an effective part of sample cleanup for more than 200 organochlorine, organophosphorus, nitrogen-containing, and carbamate pesticides, ...