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Bioanalysis Articles & Analysis
55 articles found
Traditional immunoassays based on the format of 96-well microtiter plates are widely used in analytical laboratories and have become increasingly automated with the introduction of robotics technology in recent years, thereby enhancing assay throughput. Most clinical analyzers essentially operate as "stand-alone laboratories," featuring precise, accurate, and highly reproducible programmatic ...
In Reverse Phase Chromatography, non-polar molecules have a longer retention time in the column as they have stronger interactions with the non-polar stationary phase.In practical applications, Reverse Phase HPLC is more common, especially in bioanalysis and organic molecule analysis. This is because reverse phase columns have better compatibility and separation efficiency for ...
The extinction coefficient of protein, also known as molar extinction coefficient, is a parameter that measures the absorbance capability of protein at a specific wavelength. It is crucial for bioanalysis, especially in the determination of protein concentration, assessment of protein purity, and the study of protein interactions with other molecules.DefinitionThe protein ...
Antibody drug glycosylation analysis is a critical bio-pharmaceutical analysis method, specifically used to evaluate and monitor the glycan structures on antibody drugs. Glycosylation analysis is crucial for ensuring the safety, efficacy, and consistency of drugs, as the structure and composition of glycans directly influence the bioactivity and pharmacokinetic properties of antibody ...
The pI detection of antibodies is a key technique in bioanalysis. It helps reveal the charge distribution characteristics of proteins and holds significant implications for the separation, purification, stability research, and drug research and development of proteins. ...
Metabolomics is developing rapidly. In medical science research, it is hoped that analysis to identify abnormal metabolic pathways and characteristic biomarkers closely related to diseases will provide a basis for further elucidation of disease pathogenesis. Metabolomics Research Methods Metabolomics collects samples through established metabolomics research methods, and subsequently uses ...
At the same time, various types of new methods and technologies have also emerged, such as droplet digital PCR (ddPCR) and branched DNA(bDNA) signal amplification, which have shown certain advantages and great application potential in the field of high-precision nucleic acid drug bioanalysis. In addition, the development of some automated linkage technologies, like SPE-LC-MS, is ...
In addition to determining toxicity, FC can be used to perform target regulation and functional determination to understand the MOA during drug toxicity evaluation. 3. Application in clinical bioanalysis Cell functions, such as cytokine production and changes, intracellular signal transduction, and cell proliferation can be measured by FC. ...
G. Bracewell and C. M. Smales, Bioanalysis, 2013, 5, 123-126. 9. D. G. Bracewell, R. Francis and C. M. ...
Average drug-to-antibody ratio (DAR) and drug load distribution are two vital properties of antibody drug conjugates (ADCs). Over the years, several ADC bioanalytical methods have been developed to better estimate these properties for early development and routine quality control applications. In this article, we provide an overview of the most widely used methods for the detailed analysis of DAR ...
Pollution in coastal ecosystems is becoming increasingly problematic, causing harm to animals, humans, and the environment. It destroys animal habitats, results in bacterial and viral pathogens that cause multiple illnesses, and can reduce economic growth along coastal regions. Nitrogen (N) containing species such as nitrates or ammonium can be highly detrimental to coastal marine ecosystems. ...
Ligand binding assay (LBA)LBA is the gold standard for macromolecular bioanalysis. LBA is typically used to measure TAb and total ADC, utilizing the mAb or payload component of the ADC, respectively.1.1 LBA determination of total antibody (TAb)Total Antibody (TAb) detection monitors antibody concentrations regardless of whether the payload is attached to the antibody, i.e., fully ...
Classification of oligonucleotide drugs l ASO: single-stranded DNA or modified RNA oligonucleotides; modulates target RNA function l siRNA: siRNA can induce gene silencing l miRNA: Can regulates the translation process of mRNA l Aptamer: Specific binding to ligand proteins using three-dimensional structures l Others: saRNA can activate gene expression; TRAN, sgRNA, U1 snRNA, etc. ...
Process gas analysis for bio and hydrogen gas mixtures using new high pressure in Situ sensors. The goal of the project is the development and combination of sensors for measuring optical, thermo physical and electro magnetic properties, to achieve an in Situ high pressure concentration measuring in bio and hydrogen gas ...
This is the ultimate guide to LIMS software. The work performed in labs requires a high level of efficiency to produce accurate results. Unfortunately, lab operations not supported by management and automation tools can interfere with a lab’s critical work and decrease its ability to meet business, customer and regulatory needs and requirements. That is where LIMS software comes into ...
Abstract : What very likely constituted the largest bioanalytical round robin exercise ever conducted in modern times was undertaken in Europe, the United States, and Canada to appraise the interlaboratory reliability and reproducibility of three new cost-efficient cyst-based toxicity test procedures. Close to 500 pre-packaged toxicity kits (Toxkits) of either freshwater Brachionus ...
Abstract :Microbiotesting activities comprise one important approach to address the issue of chemical contamination and its potential impacts on water quality and biota of receiving aquatic ecosystems. Under the second Saint-Lawrence River Action Plan (1993-1998), the Centre Saint-Laurent (Environment Canada, Québec Region, Montréal) continued its development and application of ...
There are a wide variety of gases found within a pharmaceutical or medical laboratory. Many have no taste, colour or smell, which makes it difficult to tell if a gas leak is present. A gas leak from a cylinder or fixed pipe gas system poses a series risk that can cause a potentially fatal incident or hazard within a laboratory environment. The pharmaceutical industry is one of the world’s ...
Laboratory research and development uses a wide range of speciality gases and equipment. It is important that these gases are monitored regularly and accurately. A gas leak from a cylinder or fixed pipe gas system poses a series risk that can cause a potentially fatal incident or hazard within a laboratory environment. Download our guide 'Gases found within pharmaceutical ...
Abstract Effect-based methods including cell-based bioassays, reporter gene assays and whole-organism assays have been applied for decades in water quality monitoring and testing of enriched solid-phase extracts. There is no common EU-wide agreement on what level of bioassay response in water extracts is acceptable. At present, bioassay results are only benchmarked against each other but not ...