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Bioluminescence Articles & Analysis
20 articles found
Abstract Adenosine triphosphate (ATP) bioluminescence testing has become the dominant rapid sanitation verification method in food processing facilities. ...
In the landscape of biological research, bioluminescent reporter cell lines stand out as a remarkable tool, illuminating the intricacies of cellular processes. ...
Advanced Imaging Techniques Recent advances in imaging technologies, such as fluorescence microscopy and bioluminescence resonance energy transfer (BRET), enable real-time monitoring of ion channel activity in live cells. ...
Measurement of free ATP: it is measured by bioluminescence without lysis buffer. Thus, only extracellular (free) ATP is able to react with the bioluminescence enzyme. ...
The qHT-dS technique is based on the principle of bioluminescence resonance energy transfer generated by novel nanoluciferases to quantitatively detect direct interactions between proteins within living cells. ...
This new technology is based on the measurement of Vibrio fischeri bioluminescence inhibition (ISO 11348). Toxicity determination The main advantage of this equipment, compared to other laboratory assays, is the fully automation of the procedure. ...
Comparison of plate counts, Petrifilm, dipslides, and adenosine triphosphate bioluminescence for monitoring bacteria in cooling-tower waters. Water Environ Res. 81(4): 401–406. ...
This new technology is based on the measurement of Vibrio fischeri bioluminescence inhibition (ISO 11348). Toxicity determination The main advantage of this equipment, compared to other laboratory assays, is the fully automation of the procedure. ...
Further test results of the eco-toxicities of oxidized mixtures to the bioluminescent marine bacterium Vibrio fischeri demonstrated the chlorinated samples could lead to the accumulation of toxic transformation products, while chlorine dioxide oxidation and ozonation had detoxication impacts during these processes. ...
Minimal toxicity to organisms (survival of Hyalella azteca and Daphnia magna; survival, growth, and tissue metal concentration of Neanthes arenaceodentata; bioluminescence of Pyrocystis lunula) was observed during 4‐h exposure to resuspended sediments and during 4‐ to 10‐d post‐exposure recovery periods in uncontaminated water. ...
Introduction The marine bacterium Vibrio fischeri is widely used for the toxicity assessment of environmental samples. The toxicity is measured in a medium containing 2% NaCl. When the bacteria are exposed to toxic compounds, the light production is inhibited. Toxicity values are obtained after 15 and 30 minutes exposure and expressed as EC50 values, the effective concentration causing 50% loss ...
Chemical coagulation–flocculation of raw TTC wastewater had no impact on the inhibition of the bioluminescence by Vibrio fischeri (BioTox assay). Subsequent biological treatment with activated sludge without PAC resulted in BioTox inhibition-free effluent (<10% inhibition). In contrast, activated sludge treatment without PAC produced an effluent that ...
GC/MS experiments confirmed that the primary metabolite for CYP3A4 and CYP2D6 is 4‐hydroxy‐PCB‐30, and the secondary metabolite is 3‐hydroxy‐PCB‐30. Cell‐based bioassays (bioluminescent yeast expressing hER‐α) confirmed that hydroxylated metabolites are more estrogenic than PCB‐30. ...
The disinfection efficiency of peracetic acid (PAA) was investigated on three microbial types using three different methods (filtration-based ATP (adenosine-triphosphate) bioluminescence, quantitative polymerase chain reaction (qPCR), culture-based method). ...
Acute toxicity was assayed with the widely applied and standardized Vibrio fischeri bioluminescence inhibition test. During a 5-month period, raw wastewater was grab-sampled from a full-scale TTC company and treated by the different unit operations on a laboratory scale. Chemical pretreatment of the wastewater by coagulation with FeCl3 removed approx. 38% of the influent ...
Microtox rapid toxicity screening was applied in some aspects of the study after verifying a correlation between the pattern of toxic responses of T.gratilla gametes and that of the bioluminescent bacteria Vibrio fischeri. TIE Phase I manipulations revealed a toxicity profile implicating surfactants as possible toxicants. ...
The resulting delay in action may allow for undue wear on manufacturing equipment and the establishment of biofilm within the fluid delivery system. ATP detection using bioluminescent enzymes is an attractive alternative to direct microbial counting but direct analysis of metal working fluids, like most oil-containing liquids, is difficult with conventional ...
A rapid method has been developed to determine enterococci using immunomagnetic separation and bioluminescence. Small paramagnetic beads are coated with antibodies having a specific affinity to enterococci. The captured enterococci are quantified by bioluminescence. The entire procedure takes 30 minutes without a pre-enrichment step. ...
The objective of the present study was to evaluate the usefulness of bioluminescence measurement and analysis of swabs taken from the surfaces of the equipment for determination of the hygienic state of a milking machine when biofilm is present on the surfaces. ...
Quorum sensing, or the control of gene expression in response to cell density, is used by both gram negative and gram positive bacteria to regulate a variety of physiological functions including bioluminescence, swarming, antibiotic biosynthesis, plasmid conjugal transfer and the production of virulence determinants in animal, fish and plant pathogens. ...