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Cell Based Assay Articles & Analysis
16 articles found
Experimental Approaches Using CCR1 Protein Studying CCR1 requires both recombinant CCR1 protein and functional cell-based assays. Recombinant extracellular domains of CCR1 are used to: l Map ligand-binding interactions with chemokines and antibodies l Screen for small-molecule or peptide inhibitors l Generate high-affinity monoclonal ...
This synergistic approach allows for targeted delivery of the cytotoxic agent to cancer cells while sparing healthy tissues, thereby reducing side effects and enhancing therapeutic efficacy. ...
The cells can be used as model systems in phenotypic and drug screening, high content analysis, target identification, drug development, toxicology testing, gene editing studies, reporter assays. ...
The IVS proprietary technology allows the growth of engineered tissues in 3D to mimic healthy and disease-naive human tissues, which enables the gap to be bridged between cell based assays and studies using isolated organs, tissues or animal models. ...
For this kind of cellular analysis, simple biochemistry is insufficient, and cell-based assays are required; nevertheless, the specificity of the analysis enabled by biochemistry cannot be forgone, and in order to analyze cells with the same degree of molecule specificity, current cell-based ...
These Al-designed antibodies also exhibited a very high cross-binding hit rate against different Spike protein RBD region mutants in ELISA assay. The Al-designed antibodies were tested in vitro and demonstrated high potency against multiple strains in real virus neutralization assays. Methods and Data In the study of SARS-Cov-2, 1300 different historical ...
Riccarton will continue to offer biosafety testing, cell line characterization, contamination testing, bioassays, and vaccine safety assays, along with lot release and stability assays. Expanding the site also allows us to better support the growing needs of an ever-changing cell and gene therapy market. Combining our experience ...
Abstract We previously generated 32 rotavirus-specific (RV-specific) recombinant monoclonal antibodies (mAbs) derived from B cells isolated from human intestinal resections. Twenty-four of these mAbs were specific for the VP8* fragment of RV VP4, and most (20 of 24) were non-neutralizing when tested in the conventional MA104 cell–based ...
We successfully integrated virtual screening methods and cell based assays into a simple, efficient procedure and identified compounds that interfere with the PCSK9-LDLR interaction. ...
We have successfully identified small molecule antagonists against PCSK9 through a virtual screen coupled with in vitro and cell-based assays. Our lead compound, P-4, exhibited a concentration-dependent inhibition of the PCSK9/LDLR interaction with an IC50 in the nanomolar range, an increase in the level of LDLR in recombinant ...
Abstract Effect-based methods including cell-based bioassays, reporter gene assays and whole-organism assays have been applied for decades in water quality monitoring and testing of enriched solid-phase extracts. ...
To that end, we have utilized a virtual screen approach coupled with cell-based assays to identify small molecule hits against the PCSK9 surface where the LDLR-EGF-A domain binds. ...
AbstractArxula adeninivorans-based yeast cell assays for the detection of steroid hormones demonstrated their efficiency for the determination of total hormone activity in a variety of samples using a microtiter plate format. In this study, a preliminary chromatographic separation using thin-layer chromatography plates is introduced in order to ...
Monoclonal antibodies (mAbs) have become an important and highly effective class of biopharmaceuticals.To identify mAbs with ideal specificity, affinity, and binding kinetics, hundreds of candidates have to be purified to high purity and screened in downstream functional assays. We describe a highly robust and reproducible, fully automated mAb purification process that allows ...
Furthermore, these compounds exhibited a 5- to 10-fold LDLR upregulation at 1.6 μM in two recombinant cell-based assays as well as exhibited an increase in the fluorescently labeled DiI-LDL uptake in situ in the nanomolar range. ...
The linchpin of the vision is to develop in vitro biochemical and cell-based assays in lieu of rodent screening assays and integrate them into its testing program. ...