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Chromatography Column Articles & Analysis
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Introducing groups such as cinnabar, ether, ester, and fluorine on the surface of nanocellulose, after chemical modification, it is used as a new type of fine chemical product in the filling material of liquid chromatography columns. The suspension of nanocellulose will be oriented under the action of magnetic field or low shear force, and this orientation still ...
In amino acid analysis, ion-exchange chromatography and reverse-phase high-performance liquid chromatography are commonly used.(2) OperationThe sample is first properly prepared, such as protein hydrolysis and elution. Then, the sample is injected into the chromatographic column. Under the transport of the mobile phase, different amino acids will ...
Mass spectrometry (MS) is ever-present in gas analysis. It is a go-to solution for precisely identifying and quantifying gas components. MS works by ionizing gas molecules and separating the resultant charged particles by their mass-to-charge ratios (m/z) in an electric or magnetic field. This allows for the detection of even trace substances with outstanding sensitivity and specificity. Mass ...
Accuracy and repeatability are central to gas chromatography. They ensure the reliability of results, effectively underscoring the trustworthiness of your measurements. An accurate measurement reflects true sample composition, while repeatability enables you to reproduce those truly representative results consistently. Without accuracy and repeatability, data integrity is compromised, leading to ...
Optical Impuritiesracemization, non-enantiomeric impurity.Common Techniques for Impurity Detection in Peptide Drugs1. High-Performance Liquid Chromatography (HPLC)HPLC has become the most commonly used method in drug quality analysis. ...
Peptide SeparationThe separation step is usually carried out using liquid chromatography (LC) technology. This process effectively reduces the complexity of the sample and improves the sensitivity of mass spectrometry analysis. Peptides are separated in the chromatography column based on their hydrophilicity or lipophilicity.3. Mass Spectrometry ...
This method has a high throughput but is only suitable for shorter oligonucleotides, and an additional step to remove the DMT group is needed after purification.1. OPC Column PurificationIt uses the affinity of the column material and the DMT at the 5′ end of the synthesized primer to specifically adsorb the oligonucleotide molecules containing DMT. ...
PrinciplesIt is mainly based on the difference in the interaction between the thiol group and the fixed phase or the mobile phase in the chromatographic column, and the thiol content is determined by chromatographic separation and detection. These include high-performance liquid chromatography and capillary electrophoresis.2. FeaturesIt has strong separation ...
These techniques can not only determine the molecular weight of small molecule peptides but also identify the sequence of peptide segments through database search and algorithm analysis.2. High-Performance Liquid Chromatography (HPLC)HPLC is a separation analysis technique that can be used for the purification and identification of small molecule peptides. By selecting the ...
High Performance Liquid Chromatography (HPLC) can be either Normal Phase HPLC or Reverse Phase HPLC, depending on the nature of the chromatographic column and the mobile phase used.Normal Phase ChromatographyIn Normal Phase HPLC, the column packing material is polar (e.g., silica gel), and the mobile phase is a non-polar or moderately polar ...
Therefore, detailed analysis and control of the monosaccharide content and types in recombinant protein vaccines are very important.To analyze the content and types of monosaccharides in recombinant protein vaccines, technologies such as High-Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) are usually required. HPLC can perform accurate analysis of the type ...
Ions are generated using techniques such as electrospray ionization (ESI) or matrix-assisted laser desorption/ionization (MALDI), and finally detected by instruments such as time-of-flight (TOF), ion trap or Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometers.2. High Performance Liquid Chromatography (HPLC)HPLC separates components by passing a sample solution ...
UV-light), physico-chemical methods {e.g. spectrophotometer and chromatography (Paper, Column, TLC, HPTLC, LC, HPLC, GLC – MS)} and immuno-enzyme methods, e.g. ...
Accurate determination of its concentration is crucial for multiple fields such as biomaterial research, food industry, and pharmaceutical research.Liquid Chromatography (LC) is an efficient and precise analytical technique for collagen concentration measurement, especially High-Performance Liquid Chromatography (HPLC) and Ultra-High Performance Liquid ...
Protein Expression and Purification(1) Express the GST fusion protein in an appropriate host cell (usually E. coli).(2) Perform affinity chromatography purification using a glutathione agarose column.(3) Check the purity and integrity of the purified protein, usually through SDS-PAGE gel electrophoresis.2. ...
Since the late 1940s, Ion Exchange Chromatography (IEC) technology has been widely used in protein separation operations. ...
Understanding the isoelectric point of antibodies is crucial for devising optimal conditions for electrophoresis, ion exchange chromatography, and other biological analysis methods, as well as determining the protein's stability and solubility behavior. ...
The commonly used methods are chemical method, column chromatography and so on. Chemical method uses chemical reagents to react with the interferents, and to decompose or transform them into substances that are favorable for PCBs determination, so as to achieve the purpose of removing the interference. The common chemical reagents are concentrated sulfuric acid ...
The commonly used methods are chemical method, column chromatography and so on. Chemical method uses chemical reagents to react with the interferents, and to decompose or transform them into substances that are favorable for PCBs determination, so as to achieve the purpose of removing the interference. The common chemical reagents are concentrated sulfuric acid ...
The basic principle of chromatography is the differential interaction between a sample mixture and a stationary phase packed inside a column. Common types of chromatography include gas chromatography (GC), liquid chromatography (LC), and thin-layer chromatography (TLC). Gas ...