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Chromatography Uplc Articles & Analysis

13 articles found

Comprehensive Insights into Tryptamine Analysis and Its Biological Significance

Comprehensive Insights into Tryptamine Analysis and Its Biological Significance

Chromatographic Techniques High-resolution separation methods such as High-Performance Liquid Chromatography (HPLC), Ultra-Performance Liquid Chromatography (UPLC), and Gas Chromatography (GC) are standard techniques for tryptamine analysis. Coupling chromatography with innovative stationary phases and gradient ...

ByCreative Proteomics


Understanding Plant Hormones: Their Role, Extraction, and Detection   

Understanding Plant Hormones: Their Role, Extraction, and Detection  

The multiple reaction monitoring (MRM) mode is frequently employed in these analyses, allowing for the simultaneous detection of multiple hormones in a single sample. Ultra-performance liquid chromatography (UPLC) is another advanced technique that, when combined with mass spectrometry, greatly improves the efficiency of hormone analysis. UPLC ...

ByCreative Proteomics


Standard Protein Detection in Mass Spectrometry Analysis

Standard Protein Detection in Mass Spectrometry Analysis

Sample Cleanup Use a C18 column or other methods to remove impurities in the sample. Liquid Chromatography Separation (LC) Use a liquid chromatography system (like UPLC) to separate the peptide segments to reduce the complexity of the mass spectrometry and increase the detection sensitivity. ...

ByMtoZ Biolabs


High Performance Liquid Chromatography (HPLC) and Pharmaceutical Analysis

High Performance Liquid Chromatography (HPLC) and Pharmaceutical Analysis

Depending on the mechanism of action, various HPLC techniques such as adsorption chromatography, partition chromatography (normal phase, reversed phase), ion exchange chromatography, affinity chromatography, gel filtration chromatography (also known as size exclusion chromatography) and gel ...

ByCD Formulation


Introduction and Detection Methods for Phytochemicals

Introduction and Detection Methods for Phytochemicals

Combined with the excellent separation capability of ultra-performance liquid chromatography (UPLC) and the high selectivity of tandem mass spectrometry (MS/MS), the detection of complex sample matrices can effectively reduce the interference of detection, thus improving the sensitivity of phytohormone detection and ensuring the reliability of analytical results. ...

ByCreative Proteomics


Lactobacillus paracasei KBL382 administration attenuates atopic dermatitis by modulating immune response and gut microbiota

Lactobacillus paracasei KBL382 administration attenuates atopic dermatitis by modulating immune response and gut microbiota

(B) Analyses of nonvolatile acids and short-chain fatty acid using gas chromatography. (C) Analyses of amino acids using ultra-performance liquid chromatography. ...

ByKoBioLabs


Stainless Steel Capillary Tubes

Stainless Steel Capillary Tubes

Ultra-small-diameter capillary is very important for high performance and ultra-high performance liquid chromatography (HPLC and UPLC) equipment. Generally, the outer diameter of such capillaries is less than 1/16 (1.59 mm), which can be used in many key applications. ...

ByWenzhou Huashang Steel Co., Ltd


Administration of Lactobacillus fermentum KBL375 Causes Taxonomic and Functional Changes in Gut Microbiota Leading to Improvement of Atopic Dermatitis

Administration of Lactobacillus fermentum KBL375 Causes Taxonomic and Functional Changes in Gut Microbiota Leading to Improvement of Atopic Dermatitis

Briefly, approximately 1 mL of cecum extracts (concentration: 20 mg/mL) in liquid chromatography-grade methanol were derivatized by 70 μL of AccQ•Tag™ Ultra Borate Buffer (Waters Corporation., Milford, MA, USA) and 20 μL of AccQ•Tag™ Ultra reagent (Waters Corporation) for 10 min at 55°C. Samples were subsequently analyzed using an Acquity ...

ByKoBioLabs


Identification and risk assessment of human and veterinary antibiotics in the wastewater treatment plants and the adjacent sea in Tunisia

Identification and risk assessment of human and veterinary antibiotics in the wastewater treatment plants and the adjacent sea in Tunisia

In the following study, we came up with and validated a prompt, sensitive and precise method for the simultaneous determination of 56 antimicrobial drugs (tetracyclines, sulfonamides, β-lactams, macrolides and quinolones) using the ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). This method was implemented with success to ...

ByIWA Publishing


Photodegradation of the benzothiostrobin in solution and on soil and glass surface

Photodegradation of the benzothiostrobin in solution and on soil and glass surface

Three photodegradation products of benzothiostrobin were identified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The photodegradation rates of benzothiostrobin increased with decreasing initial concentration of benzothiostrobin. ...

ByIWA Publishing


Impact of polymeric membrane filtration of oil sands process water on organic compounds quantification

Impact of polymeric membrane filtration of oil sands process water on organic compounds quantification

The use of ultra pressure liquid chromatography-high resolution mass spectrometry (UPLC/HRMS) showed insignificant adsorption of NAs on the tested membranes at pH 8.7. ...

ByIWA Publishing


Occurrence and removal efficiency of six polycyclic aromatic hydrocarbons in different wastewater treatment plants

Occurrence and removal efficiency of six polycyclic aromatic hydrocarbons in different wastewater treatment plants

Occurrence and removal efficiency of six polycyclic aromatic hydrocarbons (PAHs) (fluoranthene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, benzo[ghi]perylene and indeno[1,2,3-cd]pyrene) in wastewater were monitored at different treatment processes in three wastewater treatment plants (WWTPs) by using ultra performance liquid chromatography-tandem mass ...

ByIWA Publishing


High performance liquid chromatography–tandem mass spectrometry for rapid and sensitive analysis of six polycyclic aromatic hydrocarbons in wastewater

High performance liquid chromatography–tandem mass spectrometry for rapid and sensitive analysis of six polycyclic aromatic hydrocarbons in wastewater

A sensitive and fast method was developed to quantitatively analyse the six polycyclic aromatic hydrocarbons (fluoranthene (FLT), benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF), benzo[a]pyrene (Bap), benzo[ghi]perylene (BghiP) and indeno[1,2,3-cd]pyrene (INPY)) by high performance liquid chromatography (UPLC) coupling with tandem mass spectrometry ...

ByIWA Publishing

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