Cyst Based Toxicity Tests Articles & Analysis
17 articles found
Abstract : The acute toxicities of the first 50 chemicals (dextropropoxyphene hydrochloride excluded) of the multicentre evaluation of in vitrocytotoxicity (MEIC) programme were determined on four aquatic invertebrates and a bacterial strain (Photobacterium phosphoreumfor the MicrotoxTM test) commonly used in ecotoxicology testing. Three of the ...
Abstract : This paper statistically evaluates the sensitivity of the acute Daphnia magna bioassay in comparison to that of two crustacean microbiotests : the Streptoxkit F and the Thamnotoxkit F, which make use of larvae of the anostracans Streptocephalus proboscideus and Thamnocephalus platyurus respectively, hatched from cysts. Regression equations were calculated for 146 data pairs, taken from ...
Although there are several groups of aquatic invertebrates which produce resting stages, the brine shrimp Artemia(Crustacea, Anostraca) was the very first organism used in toxicity tests starting from cysts. Considering the need for simple but at the same time reliable test methods for toxicity screening in ...
Abstract :A new method is presented for determination of the acute toxicity of chemicals and effluents to larvae of the freshwater anostracanStreptocephalus proboscideus(Frauenfeld), using resting eggs (cysts) as the source of test organisms. The larvae are obtained by controlled hatching of standard reference eggs. For the ...
Abstract : The first part of the paper addresses the state of the art of standard freshwater invertebrate toxicity tests for regulatory purposes, with details of the characteristics and the test procedures of the acute and chronic tests with Daphnids (Daphnia magna, Daphnia pulexand Ceriodaphnia dubia). Subsequently this review ...
Abstract : The newly developed 1 h enzymatic inhibition bioassay (Fluotox) was applied to toxicity testing with the larvae of the brine shrimp Artemia. The method consists of visual observation of in vivo inhibition of an enzymatic process using a fluorigenic enzyme substrate. The 1 h Fluotox test and the conventional 24 h and 48 h LC50 toxicity tests were conducted in parallel on 8 different ...
Abstract : In the framework of the multicentre evaluation ofin vitrocytotoxicology (MEIC) programme, the first ten chemicals of the prescribed list were tested for acute toxicity in four standardised cyst-based aquatic invertebrate tests, consisting of two rotifer species (the estuarineBrachionus plicatilisand ...
Abstract : What very likely constituted the largest bioanalytical round robin exercise ever conducted in modern times was undertaken in Europe, the United States, and Canada to appraise the interlaboratory reliability and reproducibility of three new cost-efficient cyst-based toxicity test procedures. Close to 500 pre-packaged ...
Abstract : The determination of the toxicity of effluents and wastes at the aid of biological tests (bioassays) is hampered very seriously by the necessity of continuous culturing or maintenance of live stocks of test organisms in sufficient numbers and in a healthy state. ...
Abstract : The acute toxicity data of the first 50 chemicals of the Multicentre Evaluation of In Vitro Cytotoxicity (MEIC) programme is compared for three cyst-based toxicity tests (Artoxkit M with Artemia salina, Streptoxkit F with Streptocephalus proboscideusand Rotoxkit F with Brachionus calyciflorusand two ...
For both bioassays, test animals are obtained by hatching cysts wich eliminates the need for the culturing and the maintenance of the organisms. ...
Abstract : During the last two decades microbiotests have been developed which are independent of recruitement, maintenance and/or culturing of live stocks of test organisms. Culture and maintenance free microbiotests have been worked out in the Laboratory for Biological Research in Aquatic Pollution at the University of Ghent, with selected aquatic invertebrates. The new approach is based on the ...
Abstract: As important members of zooplankton communities worldwide, rotifers are used extensively in ecotoxicological research. Chronic rotifer tests are, however, dependent on live algal food which adds to the complexity, the variability and the costs of these bioassays. To bypass the former problem, experiments have been undertaken with the freshwater rotifer Brachionus calyciflorus, to ...
Based on preliminary investigations, a number of test parameters were investigated for a short-chronic assay : the hatching time, the size of the test cups, the feeding of the test organisms prior to the test, the amount of supplemental algal food, the volume of sediment and the duration of the ...
Abstract: In view of the need for rapid and low cost solid phase assays for contaminated sediments, a new 6 days “culture/maintenance free” microbiotest has been developed at the Laboratory for Biological Research in Aquatic Pollution at the Ghent University in Belgium, with the freshwater ostracod crustacean Heterocypris incongruens. The sensitivity of the Ostracodtoxkit FTM has ...
Abstract: Experiments have been carried out with neonates of the freshwater ostracodHeterocypris incongruensto verify and complete former choices of test parameters for a new culture/maintenance free solid phase microbiotest for freshwater sediments. From the trials with increasing volumes of reference sediment, it was concluded that 300 µL was the most appropriate amount of sediment to be ...
Abstract: The potential of the larvae of the freshwater anostracan crustaceanThamnocephalus platyurushas been evaluated as an alternative to those of the related speciesStreptocephalus proboscideus, used so far as test organism in the standard Streptoxkit F test. In line with the former cyst-based toxicity ...