Liquid Chromatography Uplc Articles & Analysis
13 articles found
Techniques like protein precipitation, liquid-liquid extraction (LLE), and solid-phase extraction (SPE) are commonly used to isolate and enrich target compounds from biological or food matrices. 2. Chromatographic Techniques High-resolution separation methods such as High-Performance Liquid Chromatography (HPLC), ...
The multiple reaction monitoring (MRM) mode is frequently employed in these analyses, allowing for the simultaneous detection of multiple hormones in a single sample. Ultra-performance liquid chromatography (UPLC) is another advanced technique that, when combined with mass spectrometry, greatly improves the efficiency of hormone analysis. ...
Sample Cleanup Use a C18 column or other methods to remove impurities in the sample. Liquid Chromatography Separation (LC) Use a liquid chromatography system (like UPLC) to separate the peptide segments to reduce the complexity of the mass spectrometry and increase the detection sensitivity. ...
High performance liquid chromatography (HPLC) has become an essential technique in pharmaceutical analysis for the separation, identification, and quantification of pharmaceuticals. ...
Combined with the excellent separation capability of ultra-performance liquid chromatography (UPLC) and the high selectivity of tandem mass spectrometry (MS/MS), the detection of complex sample matrices can effectively reduce the interference of detection, thus improving the sensitivity of phytohormone detection and ensuring the reliability of ...
(B) Analyses of nonvolatile acids and short-chain fatty acid using gas chromatography. (C) Analyses of amino acids using ultra-performance liquid chromatography. ...
Ultra-small-diameter capillary is very important for high performance and ultra-high performance liquid chromatography (HPLC and UPLC) equipment. Generally, the outer diameter of such capillaries is less than 1/16 (1.59 mm), which can be used in many key applications. ...
Briefly, approximately 1 mL of cecum extracts (concentration: 20 mg/mL) in liquid chromatography-grade methanol were derivatized by 70 μL of AccQ•Tag™ Ultra Borate Buffer (Waters Corporation., Milford, MA, USA) and 20 μL of AccQ•Tag™ Ultra reagent (Waters Corporation) for 10 min at 55°C. Samples were subsequently analyzed using an ...
In the following study, we came up with and validated a prompt, sensitive and precise method for the simultaneous determination of 56 antimicrobial drugs (tetracyclines, sulfonamides, β-lactams, macrolides and quinolones) using the ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). This method was implemented with ...
Three photodegradation products of benzothiostrobin were identified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The photodegradation rates of benzothiostrobin increased with decreasing initial concentration of benzothiostrobin. ...
However, the adsorption of AEF on the membranes increased as the pH of OSPW decreased due to hydrophobic interactions between the membrane surfaces and the protonated molecules. The use of ultra pressure liquid chromatography-high resolution mass spectrometry (UPLC/HRMS) showed insignificant adsorption of NAs on the tested membranes at pH 8.7. ...
Occurrence and removal efficiency of six polycyclic aromatic hydrocarbons (PAHs) (fluoranthene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, benzo[ghi]perylene and indeno[1,2,3-cd]pyrene) in wastewater were monitored at different treatment processes in three wastewater treatment plants (WWTPs) by using ultra performance liquid ...
A sensitive and fast method was developed to quantitatively analyse the six polycyclic aromatic hydrocarbons (fluoranthene (FLT), benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF), benzo[a]pyrene (Bap), benzo[ghi]perylene (BghiP) and indeno[1,2,3-cd]pyrene (INPY)) by high performance liquid chromatography (UPLC) coupling with tandem mass ...