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Metabolic Engineering Articles & Analysis
23 articles found
Understanding the Role of Therapeutic Enzymes Enzymes are biological catalysts that regulate numerous metabolic reactions in the body. In many rare inherited disorders, particularly metabolic diseases, genetic mutations prevent the body from producing functional enzymes. ...
Key aspects of anaerobic fermentation in food research include: l Metabolic Pathways: Microorganisms such as Lactobacillus and Saccharomyces species metabolize sugars through glycolysis, converting them into lactic acid or ethanol while regenerating NAD⁺ for continued energy production. ...
Genetic engineering allows scientists to manipulate the DNA of microorganisms, introducing new genes or altering existing ones to enhance traits such as growth rate, resistance to environmental stressors, or the ability to metabolize specific substrates. ...
Some popular areas of application of biosensors include in the food industry, for checking their quality and safety, helping to differentiate between natural and artificial; in the fermentation industry and saccharification processes, for detecting precise glucose concentrations; and in metabolic engineering, for use in Monitor cell metabolism in ...
This "untargeted" metabolomics approach is a powerful discovery engine, revealing unexpected metabolic shifts and identifying novel bioactive compounds that could be pivotal for crop improvement, drug discovery, or understanding ecological adaptation. ...
Fermentation: Under optimal temperature and pH conditions, S. cerevisiae metabolizes the sugars, producing ethanol and carbon dioxide. This stage can last from a few hours to several weeks, depending on the process being employed. ...
One essential approach to studying cellular metabolism is Metabolic Flux Analysis (MFA), a powerful tool that reveals the intracellular activities and metabolic dynamics of living systems. ...
It is particularly crucial in collagen metabolism, as it facilitates the recycling of proline, a key amino acid in the structural integrity of collagen. ...
Another emerging method involves the direct fermentation of metabolic-engineered microorganisms, utilizing natural and novel pathways to produce ethylene glycol from monosaccharides.Bio-based TPA technology is still in the developmental stage. ...
Such models play a crucial role in better understanding the pathophysiology of diabetes, evaluating novel therapeutic strategies, and predicting the short- and long-term outcomes of different treatments on glucose metabolism and diabetes-related complications. Metabolic Disease Models Metabolic diseases encompass a wide range of conditions ...
These bioorthogonal reactions find extensive applications in various fields, including radionuclide conjugate, metabolic engineering, drug target identification, medicinal chemistry, and more. ...
At the same time, it is impossible to predict the effects of mutations on other metabolic processes in an organism. In this case, recombinant DNA technology (RDT) was developed. ...
Predicting metabolic models in the early stage of drug discovery is of great significance to improve the success rate of drug discovery. ...
Recently, Gregory Stephanopoulos, the Willard Henry Dow Professor in Chemical Engineering at MIT; postdoc Amit Kumar PhD ’10, also of the Department of Chemical Engineering; and their team released a paper in the Proceedings of the National Academy of Sciences. ...
However, the application of biosurfactants is limited by the availability of economic biosurfactants and the corresponding producers that can work effectively. Hyperproducers generated by metabolic engineering of biosurfactant producers are highly desired to overcome this obstacle. ...
Department of Energy (DOE) and the National Science Foundation (NSF) to "develop a roadmap of necessary advances in basic science and engineering capabilities, including knowledge, tools and skills" to accelerate the advanced manufacturing of chemicals using biological systems. Thirteen committee members with expertise in synthetic biology, metabolic ...
Despite these impediments and although microalgae are not superior to higher plants in terms of photosynthetic efficiency and productivity, microalgal cultures remain one of the most attractive sources of feed, food and next-generation biofuels since microalgae can be grown in saline or seawater on nonarable lands, can use fertilizers with an almost 100% efficiency, are able to attain much higher ...
This problem has been addressed by metabolic engineering, which has extended endogenous insect cell N-glycosylation pathways and enabled glycoprotein sialylation by baculovirus/insect cell systems. ...
Here we describe the use of a chimeric translational fusion of yeast trehalose-6-phosphate synthase (EC 2.4.1.15) and trehalose-6-phosphate phosphatase (EC 3.1.3.12) genes to engineer stress tolerance in alfalfa. The gene fusion was overexpressed using either the constitutive 35S promoter or the stress-regulated rd29A promoter. ...
We report pyruvate formation in Escherichia coli strain ALS929 containing mutations in the aceEF, pfl, poxB, pps, and ldhA genes which encode, respectively, the pyruvate dehydrogenase complex, pyruvate formate lyase, pyruvate oxidase, phosphoenolpyruvate synthase, and lactate dehydrogenase. The glycolytic rate and pyruvate productivity were compared using glucose-, acetate-, nitrogen-, or ...