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Nucleic Acid Analysis Articles & Analysis
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This article explores the significance, applications, and methodologies related to the use of biotinylated nucleic acids What Are Biotinylated Nucleic Acids? Biotin is a water-soluble B-vitamin (Vitamin H) that can be covalently attached to various biomolecules, including nucleic acids. ...
Luminex multiplex assays is a multi-functional, multi-indicator parallel analysis system that organically integrates color-coded beads, laser technology, applied fluidics, the latest high-speed digital signal processor and computer algorithms to create a high degree of specificity and sensitivity for multi-factor detection. It can be widely used in immunoassay, ...
Among these proteins, the 108 kDa glycoprotein FHV-1 gB has a structure similar to a heterodimer. Amino acid sequence analysis shows that it has two intracellular protease cleavage sites (RTRR/S and RSRR/S), one or two of these sites can be cleaved, and this enzymatic cleavage affects the spread of the virus between cells. The gB protein is encoded by 948 amino ...
The workflow of viral metagenomics includes sample collection, virus-like particle purification, nucleic acid extraction, library preparation, and metagenomics sequencing. ...
As an efficient optical "molecular ruler", fluorescence resonance energy transfer (FRET) has a wide range of applications in biological macromolecular interactions, immunoassays, and nucleic acid detection. In the field of molecular biology, this technique can be used to study protein-protein interactions under physiological conditions in living cells. ...
Another common use of microchips in biochemistry are surface plasmon resonance assays intended for biochemical interaction analysis. Recent studies report the use of microfluidic chips as flow microreactors for enzymatic biosynthesis [2]. On-chip flow cytometry and mass spec platforms have also been developed, significantly reducing reagent consumption needed for conventional ...
Viruses were recovered from the samples using a glass wool adsorption-elution method and then further concentrated using polyethylene glycol/sodium chloride precipitation. After automated nucleic acid extraction, samples were analysed for HAV by real-time reverse-transcriptase polymerase chain reaction. HAV strains were genotyped by nucleotide sequence ...
The molecular-weight distribution and carbohydrate analysis indicated that an increase in SMP production may be partly attributed to the release of extracellular polymeric substances (EPS) into the bulk solution resulted from cerium toxicity, and the nucleic acids analysis suggested that increased cell lysis also contributed to ...