Nucleic Acid Detection System Articles & Analysis
12 articles found
Biotinylated nucleic acids are increasingly recognized as powerful tools in molecular biology and genetic research. The incorporation of biotin into nucleic acid molecules, such as DNA and RNA, allows for enhanced detection, isolation, and manipulation of these biomolecules. This article explores the significance, applications, and methodologies related to the use of biotinylated nucleic acids ...
Currently, nucleic acid aptamers are screened from large random nucleic acid libraries using SELEX technology. ...
However, there is a risk of bacterial, viral, or mycoplasma contamination during production, especially mycoplasma, a tiny bacteria without a cell wall that can pass through regular bacterial filtration systems and is difficult to detect in cell culture. Because mycoplasma contamination can potentially affect the safety and efficacy of the produced antibody ...
Introduction of OligonucleotidesOligonucleotide-based therapeutics represent a rapidly advancing field in current drug development. According to their therapeutic mechanisms, oligonucleotide drugs can be categorized into antisense oligonucleotides (ASOs), small interfering RNA (siRNA), microRNA, anti-gene transcription factor inducers, and nucleic acid aptamers. Oligonucleotide sequences ...
Once injected into the body, microspheres are gradually degraded in the body, allowing the drug to be released slowly at a certain rate, maintaining the drug concentration in the blood at the site of the lesion, thereby prolonging the half-life of the drug and achieving long-acting sustained release. Fluorescent Microspheres for Nucleic Acid ...
Epigenetics is an emerging field of research that focuses on the effects of environmental factors, such as infections, pollutants, stress, and long-term drug exposure, on an individual's genome. Epigenetic changes do not alter the structure of DNA, but do alter the way DNA is modified. Thereby, those changes include DNA methylation, histone modifications and non-coding RNAs. affects gene ...
What is lyophilization? Lyophilization in biotechnology is the process of freeze-drying, removing moisture content from a product at low temperature until it is no longer biologically or chemically active. Microbes, proteins, pharmaceuticals, plasma, and tissues are just a few examples of the many heat-sensitive things that can be preserved with the freeze-drying technique. Lyophilization ...
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Principle of Luminex Multiplex Assay Luminex xMAP technology uses polystyrene microspheres of 5.6 microns in diameter dyed with different ratios of two red-sorting fluorescent dyes to obtain up to 100 fluorescent coded microspheres. Antibody molecules or gene probes for different test items are covalently cross-linked to specific coded microspheres, each of which corresponds to a specific test ...
As an efficient optical "molecular ruler", fluorescence resonance energy transfer (FRET) has a wide range of applications in biological macromolecular interactions, immunoassays, and nucleic acid detection. In the field of molecular biology, this technique can be used to study protein-protein interactions under physiological conditions in living cells. Protein-protein interactions play an ...
Overview Catalogue Number: SCT124 Description: GelGreen Nucleic Acid Stain 10000X DMSO General Description GelGreen is a sensitive, stable and environmentally safe green fluorescent nucleic acid stain specifically designed for gel staining. GelGreen has a UV absorption between 250 nm and 300 nm and a strong absorption peak centred around 500 nm. Therefore, GelGreen is compatible with a ...
Natural waters may contain viruses, which pose a risk if water is used as raw water for drinking water or for recreational purposes. Norovirus is one of the most common causative agents for waterborne gastroenteritis infections due to its stability in the environment and due to a low infectivity dose needed to cause infection. For several years, evidence has been collected that suggests that ...
Carbon-nanotubes (CNT) represent an important group of nanomaterials with useful properties that enable their use for many important applications and made them extremely attractive for the task of electrochemical detection. Here, the author uses CNT to enhance the sensitivity of a multi-enzyme-based electrochemical bioassay for breast cancer gene (BRCA1) detection. Giant enzyme-based ...