Nucleic Acid Method Articles & Analysis
12 articles found
This article explores the significance, applications, and methodologies related to the use of biotinylated nucleic acids What Are Biotinylated Nucleic Acids? Biotin is a water-soluble B-vitamin (Vitamin H) that can be covalently attached to various biomolecules, including nucleic acids. ...
Affinity Chromatography Affinity chromatography is a specialized form of liquid chromatography that exploits the specific binding characteristics between a target molecule and a stationary phase. This method is particularly valuable in the purification of biomolecules, such as proteins, enzymes, and antibodies. ...
Because mycoplasma contamination can potentially affect the safety and efficacy of the produced antibody drugs, strict mycoplasma detection becomes extremely important.Common methods for mycoplasma detection include: isolation culture method, indicator cell method, and nucleic acid method. ...
Therefore, mycoplasma testing of recombinant protein drugs is a necessary quality control step.Common methods of mycoplasma detection include: isolation culture method, indicator cell method, and nucleic acid method. ...
In transient transfection, the introduced nucleic acid only exists in the cell for a limited time and does not integrate into the genome. ...
Hybridization-ELISA is a method that utilizes nucleic acid sequences labeled with enzymes (such as horseradish peroxidase, HRP) to specifically recognize target sequences. ...
Cell lysates were prepared as described in the “Preparation of cell lysates and WES™ analysis” section for WES™ analysis. 2.4 Virus titer determination The viral titers of IAV and SARS-CoV-2 viruses were determined using a real-time one-step multiplex RT-qPCR method. Briefly, viral nucleic acid extraction was performed ...
The workflow of viral metagenomics includes sample collection, virus-like particle purification, nucleic acid extraction, library preparation, and metagenomics sequencing. ...
The technology fixes high-density designed oligonucleotide or cDNA sequence dot matrix on the chip surface, labeling fluorescent probes for nucleic acid hybridization, and detecting the hybridization signal by laser confocal scanning microscope, to obtain nucleotide sequence information. ...
Its oocysts possess a robust wall that is extremely resistant to the chlorine used for potable water disinfection. The current procedures of nucleic acid extraction and purification, such as the freeze–thaw (F/T) method and the commercial kits, are time consuming and expensive. To this end, a surfactant extraction treatment (SET) was developed as ...
Later, the monitoring was continued for some winter-spring months for norovirus and adenovirus. Viral nucleic acids were detected using molecular methods, such as real-time reverse transcription-polymerase chain reaction. ...
Further, SEL was able to resuscitate acid- and cold-stressed cells, and recovery was comparable to that in nonselective tryptic soy broth containing 6% yeast extract but superior to that in the respective individual selective broths. ...