Pcr System Articles & Analysis
6 articles found
Threshold method This method quantitatively detects ssDNA to calculate the total amount of DNA in a sample, is limited to detecting DNA fragments less than 800 bp, and may be inhibited by high concentrations of DNA (1 ng/ml), and is also susceptible to short DNA fragments (20–80 bp) ) and lack stability. Quantitative PCR for Residual Host Cell DNA Testing At present, and ...
Moreover, an artificial exogenous QuantiNova RNA internal control (Qiagen, Venlo, Netherlands) was included during the nucleic acid purification to monitor the RNA purification efficiency and the RT-PCR amplification. A Rotor-Gene Q™ Real-Time PCR detection system (Qiagen, Venlo, Netherlands) was used for amplification, and the data were ...
In competition with bacteriophages, bacteria and archaea have evolved unique ways of defending, which include the CRISPR/Cas systems and the immune systems of bacteria and archaea, which resist the invasion of foreign DNA or RNA and recognize the foreign invaders and cleave them for immune defense. CRISPR/Cas systems are now useful research tools ...
As the world opens up over the coming months and years, there will be countless plane journeys to see loved ones, meet new family members and travel for work and leisure. Many of these travellers will first need to provide evidence of a negative PCR test to show they pose a minimal COVID-19 transmission risk. Time is proving of the essence for testing. With reports of multi-hour queues at ...
In this study, we developed a real-time PCR detection system based on gyrase B subunit genes (gyrB) specific to Bacteroides fragilis. The gryB-based method was compared with previously described 16S rRNA-based real-time qPCR methods and evaluated for specificity, sensitivity and robustness in detecting B. fragilis from untreated recreational water impacted by ...
Community diversity and abundance of biofilms from a full-scale drinking water distribution system in Shanghai were characterized by denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA sequences and heterotrophic plate count (HPC), respectively. ...