Refine by
Process Mass Spectrometry Articles & Analysis
55 articles found
Process optimisation thus requires robust fermentation monitoring to safeguard yields and process consistency. Mass spectrometry plays a crucial role in fermentation monitoring. It provides the real-time analysis of gases and dissolved gas species in line. ...
Its principle involves obtaining peptides from protein samples and then assembling these peptides into a complete protein sequence. This process requires the use of mass spectrometry technology to digest protein samples into peptides, followed by determining the mass and sequence information of these peptides via ...
This experiment requires strict adherence to a series of precautions to ensure the accuracy and repeatability of the data.Sample HandlingSample collection, storage, and processing must be standardized to avoid freeze-thaw cycles. Samples should be kept free from contamination to maintain their purity.Sample Separation and PreparationAppropriate separation methods, such as ...
Studying the structure and function of these tissues is of great significance for understanding life processes, disease mechanisms, and medical research.Main Research Content and Methods of Proteomics in Animal Tissues1. ...
TMT (Tandem Mass Tags) technology is an advanced method used for protein quantification analysis in proteomics research. ...
A lot can be learned about compounds and molecules by assessing their molecular masses and the make-up of their structures. This knowledge can provide an understanding of a compound’s characteristics, such as its solubility and reactivity, and help enforce quality control, for instance in pharmaceutics, by identifying contaminants and impurities. A technique used to quantify the ...
The process of IP-MS (Immunoprecipitation Tandem Mass Spectrometry Analysis) mainly includes the following steps.Analysis Workflow1. ...
Currently, commonly used protein sequence analysis methods mainly include terminal sequencing methods (such as Edman degradation method and carboxypeptidase method) and mass spectrometry-based methods. Shotgun is a mass spectrometry-based protein sequencing method. ...
Mass Spectrometry AnalysisMass spectrometry analysis is an analytical technique that determines the chemical structure of compounds by measuring the mass and abundance of ions. In protein mass spectrometry analysis, mass spectrometry can be used to identify and ...
This article mainly discusses the application of mass spectrometry in the detection of host cell protein (HCP) residues.Host cell protein (HCP) refers to proteins derived from the host cells of the expression system in the process of biopharmaceutical production. ...
Peptide SeparationThe separation step is usually carried out using liquid chromatography (LC) technology. This process effectively reduces the complexity of the sample and improves the sensitivity of mass spectrometry analysis. ...
In recent years, mass spectrometry has played a significant role in the detection and identification of collagen.What is Mass Spectrometry?Mass spectrometry is an analytical technique that can determine molecular mass and molecular structure. ...
Mass Spectrometry TechnologyMass spectrometry technology directly measures the mass and structure of T cell receptor proteins to identify differences among different T cell clones. ...
During this process, protease inhibitors can be added to prevent protein degradation.Protein PurificationProtein purification is done to remove non-protein components that may interfere with mass spectrometry analysis, such as lipids and sugars. This is typically achieved through gel electrophoresis or high-performance liquid chromatography ...
Mass Spectrometry is a powerful tool for detecting and identifying molecules and their structures, which is widely used in protein mass spectrometry analysis. ...
It can identify and quantify proteins and their modifications. Quantitative mass spectrometry is particularly suitable for studying protein modifications, as it can measure the relative abundance of proteins and their modifications simultaneously.Quantitative mass spectrometry analysis has been successfully applied in linear ...
MixingAll labeled samples are mixed together so that multiple samples can be processed simultaneously in the same mass spectrometry analysis.4. Liquid Chromatography Separation (LC)The mixed peptide samples are separated by liquid chromatography to reduce sample complexity and improve the resolution and sensitivity of mass ...
Therefore, studying the changes in the high-level structure of proteins in the drug design process is crucial for understanding the mode and function of potential drugs.Hydrogen Deuterium Exchange Mass Spectrometry (HDX-MS) is a mass spectrometry technique for studying the spatial conformation of proteins. ...
Since arginylation often occurs at low abundance sites on proteins, high-sensitivity analytical techniques are required.The Steps of Mass Spectrometry for Studying Post-Translational Modifications of Proteins1. ...
Mass spectrometry analysis is a commonly used analysis method in basic and advanced drug research. ...