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MtoZ Biolabs
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MtoZ Biolabs - Model 2d-dige -2D-DIGE Based Protein Quantitative Service

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Two-dimensional difference gel electrophoresis (2D-DIGE) is a novel quantitative proteomics technique that evolved from traditional two-dimensional gel electrophoresis (2-DE). The principle of 2D-DIGE for separating mixed proteins is the same as traditional 2-DE. It uses differences in protein isoelectric points and molecular weights to separate protein mixtures. At the same time, the sensitive fluorescent dyes and the internal standard make it significantly better than traditional 2-DE in quantitative prot
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Two-dimensional difference gel electrophoresis (2D-DIGE) is a novel quantitative proteomics technique that evolved from traditional two-dimensional gel electrophoresis (2-DE). The principle of 2D-DIGE for separating mixed proteins is the same as traditional 2-DE. It uses differences in protein isoelectric points and molecular weights to separate protein mixtures. At the same time, the sensitive fluorescent dyes and the internal standard make it significantly better than traditional 2-DE in quantitative proteomics. The fluorescent dyes used in DIGE are Cy2, Cy3, and Cy5, which react with the lysine side chain amino groups of proteins to label the proteins without affecting their isoelectric points and molecular weights. After mixing equal amounts of the labeled proteins, two-dimensional electrophoresis is conducted. The internal standard Cy2 is used to match different gels and eliminate gel viriation, while changes in protein expression levels are reflected by fluorescence intensity of Cy3 and Cy5. As a classic method of quantitative proteomics, 2D-DIGE is widely applied and suitable for various samples.

Blundon, M. Methods Mol Biol. 2019.

MtoZ Biolabs provides SDS-PAGE and 2D-DIGE services. Combining Thermo Fisher's Orbitrap Fusion Lumos mass spectrometer platform and nanoLC-MS/MS nano-liquid chromatography, We provide comprehensive proteomics identification and quantification services for researchers.


Both Liquid and Solid Samples are Acceptable


Suitable for Various Samples


1. Experimental Procedures

2. Relevant Experimental Parameters

3. Gel and Mass Spectrometry Images

4. Raw Data

5. Results of the 2D-DIGE Quantitative Proteomics Analysis


MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.

MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.

Email: [email protected]