MtoZ Biolabs - Model disulfide-bond -Disulfide Bond
Disulfide bond is a covalent bond formed between the sulfur atoms of two cysteines. This bond is crucial for the stability and function of proteins, and many secreted proteins or proteins that are located at the cellular surface have formed disulfide bond to maintain the stability. To meet specific research needs of our customers, MtoZ Biolabs provides disulfide bond analysis service at both single protein level and proteome level. We have optimized our sample preparation method to reduce the chance of in vitro disulfide bond exchange and maintain the native protein structure to the greatest extent.
MtoZ Biolabs has developed a specialized platform, equipped with Q Exactive HF (Thermo Fisher), Orbitrap Fusion, and Orbitrap Fusion Lumos mass spectrometers, equipped with Nano-LC for disulfide bond analysis service. To better solve your research problems, we offer an all-inclusive service, including sample preparation, protein purification, digestion, peptide separation, LC-MS/MS analysis, pLink-SS software analysis and bioinformatics analysis. All you need to do is to tell us your project objective, and send us your cell/protein samples, and we will complete all the following experiments.
1. Mapping the Disulfide Bond Sites of Unknown Proteins
2. Verify the Disulfide Bonds and Folding of Protein Samples
1. Analysis of Proteins at Single Protein Level and Proteomics Level
2. High-Throughput, Quantitatively Identify and Analyze Disulfide Bridges of Multiple Proteins
3. Protein are Maintained and Analyzed in Native Status
Note: Please use enough dry ice in the shipment, and choose express delivery to eliminate sample degradation.
We will perform testing experiment before we start official experiments. To ensure the most cost-effective and accurate analysis is provided, only qualified samples will proceed to the official analysis.
1. Experiment Procedures
2. Parameters of Liquid Chromatography and Mass Spectrometer
3. MS Raw Data Files
4. Peptide Identifications and Intensities
5. Protein Identifications and Disulfide Bonds Mapping
6. Bioinformatics Analysis
PTM Analysis
Protein Analysis
Protein Sequencing
MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.
MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.
Email: [email protected]