MtoZ Biolabs - Model pct-dia-proteomics -PCT-DIA Proteomics Service

Pressure cycling technology (PCT) is an efficient biological sample preparation technology and a patented technology developed by Pressure BioSciences Inc (PBI), USA. It uses pressure cycling between atmospheric pressure and ultra-high (liquid) pressure (35,000 PSI or higher), repeatedly breaking cells and enabling the extraction of important biomolecules such as proteins from various samples (cells and tissues from humans, animals, plants, and microorganisms). Data-independent acquisition (DIA) is a holographic mass spectrometry data acquisition mode based on the electrostatic field Orbitrap and is a data-independent scanning mode. Compared to data-dependent acquisition mode (DDA), DIA technology divides the entire scanning range of mass spectrometry into several windows, selecting, fragmenting, and detecting all ions within each window, providing better accuracy and repeatability. The combination of PCT and DIA technologies allows the study of the proteome of paraffin-embedded or formalin-fixed tissue samples and trace samples.

MtoZ Biolabs, using Thermo's latest Obitrap Fusion Lumos mass spectrometer combined with Nano-LC nanoliter chromatography technology, offers a PCT+PRM proteomics analysis service. You only need to send us your samples, and we will take care of all subsequent project procedures, including protein extraction, protease digestion, peptide separation, mass spectrometry analysis, raw data analysis, and bioinformatics analysis.

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Mass Spectrometry Images

4. Raw Data

5. Protein Differential Level Analysis

6. Bioinformatics Analysis

MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.

MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.

Email: [email protected]