Refine by
Chromatography Separation Articles & Analysis
146 articles found
Typical Procedure Summary Step Description Key Notes Extraction Homogenize 0.5 g of fresh tissue in 3% sulfosalicylic acid Keep samples cold to avoid degradation Reaction Mix extract with acid ninhydrin and glacial acetic acid, heat at 100 °C for 1 hour Color intensity depends on proline concentration Separation Cool and extract chromophore with toluene Ensure complete ...
How IC is helping us provide more accurate, reliable, and insightful environmental solutions. Ion chromatography (IC) is an analytical chemistry technique to analyze ions and polar molecules via liquid chromatography. This is a separation technique that uses a liquid mobile phase to separate components of a mixture, based on ...
Phytochemical tests are a cornerstone in natural product research, enabling scientists to identify and characterize the bioactive compounds present in plant materials. These tests often serve as the first step in screening for potential therapeutic agents before more advanced analytical techniques are applied. While the procedures themselves are generally straightforward, achieving reliable and ...
Choosing the right eluent in chromatography is crucial—especially for ion exchange chromatography (also known as ion chromatography or IC)—as is the choice of the correct column. Both must work in harmony to achieve optimal separation performance. In chromatography, we often refer to the ...
ByB&W Tek
Analytical Methods for Fatty Acids Quantification Quantifying fatty acids typically involves the extraction, derivatization, and separation of lipid components. Among available techniques, gas chromatography-mass spectrometry (GC-MS) remains the gold standard due to its sensitivity, reproducibility, and specificity. GC-MS fatty acid profiling often starts with ...
Techniques for Plant Hormone Analysis Advanced analytical techniques are employed to measure and analyze plant hormones: Chromatographic Techniques: High-performance liquid chromatography (HPLC) and gas chromatography (GC) are widely used to separate and quantify hormones due to their specificity and sensitivity. ...
The current focus on precision and sustainable efficiency has pushed ion exchange resins beyond their traditional applications. Specialty ion exchange resins that feature custom chemical designs and properties have become essential components for industries that require precise selectivity, sturdy durability, and exceptional performance in harsh environments. Alfa Chemistry has been deeply ...
Introduction Helium is one of the most fascinating and versatile elements in the periodic table. Known for its unique properties, it plays an essential role in a broad range of industries, from aerospace to healthcare and semiconductor manufacturing. However, its precise measurement is crucial for ensuring safety, efficiency, and accuracy in its applications. In this article, we’ll ...
Identification of the Released PTH-Amino Acid: The PTH-amino acid is separated, typically using chromatography or electrophoresis, and identified based on its unique properties. ...
Popular isolation methods include: Ultracentrifugation: A gold-standard method that separates exosomes based on density. Size-Exclusion Chromatography (SEC): Allows separation of exosomes based on size, minimizing contamination. ...
Additionally, amino acid determination can also be used to monitor the levels of amino acids in biological samples, which is important for studying the pathogenesis and treatment effects of various diseases.Common Methods of Amino Acid Determination1. High-Performance Liquid Chromatography (HPLC)(1) PrincipleHPLC can separate, identify, and quantify various ...
Accurate analysis and monitoring of the components of the medium can help researchers and manufacturers optimize the production process, increase yield, and ensure the safety and effectiveness of the final product.Technologies for Analyzing the Components of Antibody Drug Culture Media1. High-Performance Liquid Chromatography (HPLC)(1) HPLC can be used to quantitatively analyze ...
Chromatography is a powerful analytical technique widely used for separating and purifying components within a mixture. ...
Ion chromatography (IC) is an analytical technique used to separate and quantify ionic and polar analytes. ...
ByB&W Tek
The measurement of collagen can help researchers and physicians understand the pathological process of related diseases (such as osteoporosis, arthritis, skin aging etc.), and also aid in drug development and other biomedical research.The measurement of collagen can usually be carried out through several different methods:Mass Spectrometry1. Proteomic AnalysisLiquid ...
Enzymatic DigestionThe protein sample is treated with an enzyme (usually trypsin) to hydrolyze the proteins into peptides.Liquid Chromatography Separation1. Peptide SeparationPeptides are separated by their hydrophobic properties by using reversed-phase high-performance liquid chromatography (HPLC) or other chromatographic techniques.Mass ...
Shotgun Quantitative Proteomics relies on the combination of High Performance Liquid Chromatography (HPLC) and Tandem Mass Spectrometry (MS/MS). Proteins are enzymatically digested into peptides, separated by liquid chromatography, and finally quantified by mass spectrometry. ...
Each tag has the same mass and structure, but slightly different composition of heavy isotopes, which generate different signals in mass spectrometry analysis.Sample Mixing and Chromatographic SeparationThe labeled samples are mixed together and then separated by liquid chromatography (LC).Mass Spectrometry AnalysisThe separated peptides are ...
This process typically goes through 10 to 20 amino acid cycles, with each cycle determining one amino acid.Substitution ReactionThe reacting labeled compound is collected and removed from the reaction vessel through acid-base neutralization.Analysis and DetectionThe collected labeled compound is analyzed and detected, usually using techniques such as high-performance liquid ...
Based on the "bottom-up" mass spectrometry analysis strategy, through the data-dependent acquisition (DDA) mode, it aims to achieve unbiased identification and quantification of proteins in biological samples.The general process of shotgun protein sequencing is to first digest the protein sample into peptides, and then separate the peptides by high-performance liquid ...