Liquid Chromatography System Articles & Analysis
27 articles found
Combined with advanced mass spectrometry and chromatography techniques, these services primarily employ two complementary approaches: untargeted lipidomics and targeted lipidomics. ...
Liquid chromatography (LC) as one of the commonly used chromatography technologies has a wide range of applications and is often used in combination with 2-DE to achieve automation of the experimental process. High-performance liquid chromatography (HPLC) has developed on the basis of LC. With its high ...
Peptide identification is a core process in proteomics, involving the analysis of proteins in biological samples to identify specific peptide segments. This process is not only crucial for basic biological research, but it also plays a key role in disease diagnosis, drug discovery, and biomarker identification. A complete peptide identification process can be approximately divided into the ...
In biological experiments, protein sequencing is a critical technique that enables the determination of the amino acid sequence of proteins, thereby revealing their function and structure. However, a common question arises: is it possible to sequence impure proteins? This paper will explore this issue.Importance of Protein PurityFirstly, let's understand why protein sequencing requires high ...
Oligonucleotides are typically composed of short-chain nucleotides (deoxyribonucleotides or ribonucleotides) of less than 20, serving as short RNA and DNA oligomers. They can be used as probes to determine the structure of DNA or RNA, and are used in processes such as gene chips, electrophoresis, and fluorescence in situ hybridization.Oligonucleotides are widely used in biology, agriculture, ...
Phosphorylation is one of the most common and important post-translational modifications of proteins, playing a key role in many cellular activities such as signal transduction, cell division, metabolic regulation, and cell death. Therefore, the accurate identification of the phosphorylation status of proteins is crucial for understanding cellular functions and disease mechanisms. 4D-label-free ...
Combined with enzymatic digestion and nano-liquid chromatography (Nano-LC) technology, the separation efficiency and identification sensitivity of the sample can be improved.2. ...
Sample Cleanup Use a C18 column or other methods to remove impurities in the sample. Liquid Chromatography Separation (LC) Use a liquid chromatography system (like UPLC) to separate the peptide segments to reduce the complexity of the mass spectrometry and increase the detection sensitivity. ...
Proteomics, as an important part of systems biology, aims to comprehensively analyze the expression, function, and interaction of all proteins in biological organisms. ...
Host cell proteins (HCPs) are process-related impurities produced by host cells and are typically present at low levels in recombinant biopharmaceutical products. Enzyme-linked immunosorbent assay (ELISA) has traditionally been used to monitor the total content of HCP in the production of therapeutic protein drugs. Now, with the continuous improvement and upgrading of technology, liquid ...
Purification: Purify the synthesized peptide by reverse-phase high-performance liquid chromatography (HPLC) using a suitable solvent system and detect the peptide using UV absorption at 214 nm. 9. ...
UV-Vis Absorption Detector Ultraviolet-visible detector (UVD) is one of the most widely used detectors in HPLC, and almost all liquid chromatographs are equipped with this detector. UVD is characterized by high sensitivity, wide linear range, low noise, and is suitable for gradient elution. ...
The following handbook will provide guidelines for instrument preparation and routine operation recommendations to maintain the outstanding life-time performance of the LC6000.IntroductionThe LC6000 Good Practice Handbook is designed for all users of SCION Instruments LC6000, High Performance Liquid Chromatography (HPLC) systems. The below set of ...
High performance liquid chromatography (HPLC) has become an essential technique in pharmaceutical analysis for the separation, identification, and quantification of pharmaceuticals. ...
· LC-MS/MS (1) The principle of the LC-MS/MS The high-performance liquid chromatography-mass spectrometry system can be used to study the molecular structure and other information by analyzing the molecular weight and elemental composition of substances. ...
What is gas chromatography and liquid chromatography? Gas chromatography is a chromatographic method that uses gas as a mobile phase. ...
What is gas chromatography and liquid chromatography? Gas chromatography is a chromatographic method that uses gas as a mobile phase. ...
Anthocyanin Definition Anthocyanins, flavones, isoflavones, flavonols, and proanthocyanidins are all flavonoids that belong to a large group of important secondary metabolites widely found in plants. Anthocyanins are found in 72 genera of flowering plants (angiosperms) in 27 families. Among them, grapes, hawthorn, pine needles, and purple sweet potatoes have high anthocyanin content. The basic ...
Ultra-small-diameter capillary is very important for high performance and ultra-high performance liquid chromatography (HPLC and UPLC) equipment. Generally, the outer diameter of such capillaries is less than 1/16 (1.59 mm), which can be used in many key applications. ...
KNAUER’s role in the IMPRESS consortium is to contribute with its expertise and liquid chromatography purification technology, including the very efficient continuous separation technology simulated moving bed (SMB) chromatography. ...