MtoZ Biolabs - Model glycosphingolipid-glycans-analysis -Glycosphingolipids Analysis Service
Glycosphingolipids are widely present in cell membranes and serum. They either exist in free form or are complexed with other proteins. The glycan head group determines the biological function of glycosphingolipids. Abnormal glycosylation of glycosphingolipids is closely related to various cancers, such as breast cancer, lung cancer, and brain tumors. Glycosphingolipid glycans are also involved in many cellular processes, such as signal transduction, receptor function, and cell differentiation. Therefore, detailed analysis of the glycan head group structure of glycosphingolipids is of great value for disease research.
Comprehensive analysis of glycosphingolipid glycans requires the use of glycosidase, which has broad substrate specificity and supports high-throughput analysis. Glycosphingolipid analysis involves the following steps:
1. Extraction and Purification of Glycosphingolipids from Cell Membranes or Serum
2. Release of Glycosphingolipid Glycans using Glycosidase
3. Permethylation of Glycans
4. UPLC-HILIC-FLD Analysis Based on 2AB Labeling
5. Analysis of Permethylated Glycans by TOF-MS
If necessary, determination of glycan structure by MS/MS.
MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.
MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.
Email: [email protected]