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MtoZ Biolabs - Model oligonucleotide-purity-analysis -Oligonucleotide Purity Analysis Service
Oligonucleotides, including DNA and RNA, have a wide range of uses in gene therapy, biological probes, and diagnosis. For example, DNA can be introduced into immune cells and be modified to express chimeric antigen receptor proteins, which can be used for cell-based immunotherapy. When oligonucleotides are synthesized by solid-phase synthesis techniques, trace impurities may be produced at various stages of the synthesis process. Therefore, the analysis and detection of oligonucleotide purity are particularly important to ensure the quality, accuracy, safety, and biological activity of oligonucleotide in its production and research processes.
Kanavarioti, A. et al. Sci Rep. 2019.
SDS-PAGE serves as a conventional method for purifying oligonucleotides by size. However, higher-resolution chromatography is required when oligonucleotides are similar size or contain modifications that hinder separation. The preferred method for oligonucleotide purification is ion-pair reversed-phase high-performance liquid chromatography (IP-RP-HPLC), which can be coupled with MS for detailed oligonucleotide mass characterization. At MtoZ Biolabs, we utilize a HPLC platform to develop and validate methods for analyzing oligonucleotide purity. These methods cater to various oligonucleotides, allowing for the identification of impurities caused by imcomplete reaction, such as n-1 deletions, n+1 additions, incomplete sulfations, and byproducts like diphosphates.Welcome to contact to learn more about our service.
1. Synthetic Oligonucleotides: Oligonucleotides such as PCR Primers and Probes
2. Natural Oligonucleotides: Oligonucleotides Isolated from RNA or DNA
3. Oligonucleotide Pharmaceuticals: Oligonucleotides Used in Gene, Antiviral, and Anticancer Therapies
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Detailed Information on Oligonucleotide Purity Analysis
4. Mass Spectrometry Images
5. Raw Data
MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.
MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.
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