MtoZ Biolabs - Model pcr-based-antibody-sequencing -PCR Based Antibody Sequencing

Although hybridoma cells are immortalized, extensive passage of hybridoma cells may cause mutations on antibody genes, and hybridomas may loss their specificity to produce the correct antibody. Therefore, sequence information of antibody is of extreme importance for stable quality of antibody in large-scale industrial manufacturing. To solve this problem, MtoZ Biolabs has developed a PCR based antibody sequencing workflow, coupled with degenerate primer design. Compared with the mass spectrometry-based antibody sequencing method, this PCR-based antibody sequencing method is much more cost effective and less time consuming. In this method, degenerate FR1 primers are designed for PCR amplification of immunoglobulin (Ig) light and heavy chain variable regions from your hybridoma extracted mRNA. To better meet your research needs, we provide a full-package service, covering the whole analytical steps involving antibody sequencing. You can simply send us your hybridoma cells, and we will perform all the following analytical experiments.

1. Sequencing of Antibodies Derived from Multiple Common Species, Including Human, Mice, Rat, etc

2. Quick Turnaround Time

3. High Quality Analysis with Competitive Price

4. Full-Package Service and Custom Service Is Also Supported

1. Amplified DNA Fragments of Light and Heavy Chain Variable Regions (Optional)

2. Experimental Procedures and All Reagents

3. DNA Sequencing Results

4. DNA Sequence Alignments of Heavy and Light Chain Variable Regions

5. DNA Gel Images

6. Analytical Raw Data Files

Protein Sequencing

Protein De Novo Seq

N-Terminal Sequencing

C-Terminal Sequencing

Edman Degradation

Protein Full-Length Sequencing

Protein Analysis

Protein Identification

Protein Mass Measurement

PTMs Identification

PTM Analysis

Phospho-Proteomics

Acetyl-Proteomics

Ubiquitin-Proteomics

Glyco-Proteomics

Disulfide Bond

Histone Modifications

MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.

MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.

Email: [email protected]